{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T10:41:50Z","timestamp":1761561710505},"reference-count":26,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2013,1,3]],"date-time":"2013-01-03T00:00:00Z","timestamp":1357171200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T00:00:00Z","timestamp":1441065600000},"content-version":"tdm","delay-in-days":971,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2013,10]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>Large granular lymphocytes (<jats:styled-content style=\"fixed-case\">LGL<\/jats:styled-content>s) are medium\u2010 to large\u2010sized lymphocytes with azurophilic cytoplasmic granules. Reactive <jats:italic>vs<\/jats:italic>. neoplastic <jats:styled-content style=\"fixed-case\">LGL<\/jats:styled-content>s are usually morphologically indistinguishable.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We investigated 25 consecutive cases of <jats:styled-content style=\"fixed-case\">LGL<\/jats:styled-content> lymphoproliferation using flow cytometric T cell receptor V\u03b2 (<jats:styled-content style=\"fixed-case\">FC<\/jats:styled-content>\u2010V\u03b2) repertoire and T cell receptor gene rearrangement (<jats:styled-content style=\"fixed-case\">TCR<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">GR<\/jats:styled-content>) in detecting clonality.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Seventeen patients (68%) were T\u2010<jats:styled-content style=\"fixed-case\">LGL<\/jats:styled-content> leukemia (T\u2010<jats:styled-content style=\"fixed-case\">LGLL<\/jats:styled-content>) with a male predominance, a median age of 67, and a median absolute <jats:styled-content style=\"fixed-case\">LGL<\/jats:styled-content> count of 2.592\u00a0\u00d7\u00a010<jats:sup>9<\/jats:sup>\/L. All cases were clonal using the <jats:styled-content style=\"fixed-case\">FC<\/jats:styled-content>\u2010V\u03b2 analysis, and all but one (94%) was clonal by <jats:styled-content style=\"fixed-case\">TCR<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">GR<\/jats:styled-content>. Eight patients (32%) had reactive <jats:styled-content style=\"fixed-case\">LGL<\/jats:styled-content> lymphoproliferation. Two had <jats:styled-content style=\"fixed-case\">EBV<\/jats:styled-content>\u2010associated infectious mononucleosis; one was clonal by both <jats:styled-content style=\"fixed-case\">FC<\/jats:styled-content>\u2010V\u03b2 and <jats:styled-content style=\"fixed-case\">TCR<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">GR<\/jats:styled-content>; and the other was clonal only by <jats:styled-content style=\"fixed-case\">TCR<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">GR<\/jats:styled-content>. The remaining six cases were polyclonal by both assays. Patients with reactive <jats:styled-content style=\"fixed-case\">LGL<\/jats:styled-content> lymphoproliferation were more frequently associated with an underlying\/concurrent malignancy than those with T\u2010<jats:styled-content style=\"fixed-case\">LGLL<\/jats:styled-content> (4\/8 cases <jats:italic>vs<\/jats:italic>. 1\/17; <jats:italic>P<\/jats:italic>\u00a0=\u00a00.023, Fisher's exact test). We compared the demographic, hemogram, and clonality data between these two groups and found that the only significant difference was the lower median platelet count in the <jats:styled-content style=\"fixed-case\">LGL<\/jats:styled-content> lymphocytosis group (201\u00a0\u00d7\u00a010<jats:sup>9<\/jats:sup>\/L <jats:italic>vs<\/jats:italic>. 223\u00a0\u00d7\u00a010<jats:sup>9<\/jats:sup>\/L; <jats:italic>P<\/jats:italic>\u00a0=\u00a00.031; Student's <jats:italic>t<\/jats:italic>\u2010test). A literature review including the current study showed a high sensitivity of <jats:styled-content style=\"fixed-case\">FC<\/jats:styled-content>\u2010V\u03b2 analysis for T\u2010<jats:styled-content style=\"fixed-case\">LGLL<\/jats:styled-content> (97.2%; 107\/110 cases).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">FC<\/jats:styled-content>\u2010V\u03b2 analysis was slightly more sensitive than <jats:styled-content style=\"fixed-case\">TCR<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">GR<\/jats:styled-content> for the detection of clonal T cell lymphoproliferation. However, we must interpret the laboratory findings with clinical context as clonal T cell lymphoproliferation may occur in patients with viral infection.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijlh.12041","type":"journal-article","created":{"date-parts":[[2013,1,3]],"date-time":"2013-01-03T10:42:41Z","timestamp":1357209761000},"page":"501-509","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["A comparative study of flow cytometric T cell receptor V\u03b2 repertoire and T cell receptor gene rearrangement in the diagnosis of large granular lymphocytic lymphoproliferation"],"prefix":"10.1111","volume":"35","author":[{"given":"Y.\u2010C.","family":"Hsieh","sequence":"first","affiliation":[{"name":"Department of Pathology Chi\u2010Mei Medical Center Tainan Taiwan"},{"name":"Department of Medical Laboratory Science and Biotechnology Chung Hwa University of Medical Technology Tainan Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.\u2010T.","family":"Chang","sequence":"additional","affiliation":[{"name":"Department of Pathology Chi\u2010Mei Medical Center Tainan Taiwan"},{"name":"Department of Nursing National Tainan Institute of Nursing Tainan Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W.\u2010T.","family":"Huang","sequence":"additional","affiliation":[{"name":"Division of Hemato\u2010Oncology Department of Internal Medicine Chi\u2010Mei Medical Center Tainan Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.\u2010Y.","family":"Kuo","sequence":"additional","affiliation":[{"name":"Department of Pathology Chi\u2010Mei Medical Center Tainan Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.\u2010A.","family":"Chiang","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory Science and Biotechnology Chung Hwa University of Medical Technology Tainan Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.\u2010S.","family":"Chuang","sequence":"additional","affiliation":[{"name":"Department of Pathology Chi\u2010Mei Medical Center Tainan Taiwan"},{"name":"Department of Pathology Taipei Medical University Taipei Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,1,3]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1634\/theoncologist.11-3-263"},{"key":"e_1_2_6_3_1","first-page":"272","volume-title":"WHO Classification of Tumours of Haematopoietic and Lymphoid Tissues","author":"Chan 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