{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,3]],"date-time":"2023-10-03T05:10:46Z","timestamp":1696309846688},"reference-count":25,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2013,4,10]],"date-time":"2013-04-10T00:00:00Z","timestamp":1365552000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T00:00:00Z","timestamp":1441065600000},"content-version":"tdm","delay-in-days":874,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2013,12]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p><jats:italic><jats:styled-content style=\"fixed-case\">ABL<\/jats:styled-content>1<\/jats:italic> kinase mutations represent a major mechanism of imatinib resistance in Philadelphia\u2010positive (Ph+) patients. There is a paucity of data on <jats:italic><jats:styled-content style=\"fixed-case\">ABL<\/jats:styled-content>1<\/jats:italic> kinase mutations in Ph+ patients in Korea.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We used restriction fragment mass polymorphism (<jats:styled-content style=\"fixed-case\">RFMP<\/jats:styled-content>) analysis to detect <jats:italic><jats:styled-content style=\"fixed-case\">ABL<\/jats:styled-content>1<\/jats:italic> kinase mutations in blood or bone marrow specimens from 80 Ph+ patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Fifty\u2010seven patients met the criteria for inadequate molecular response (<jats:styled-content style=\"fixed-case\">IMR<\/jats:styled-content>). <jats:italic><jats:styled-content style=\"fixed-case\">ABL<\/jats:styled-content>1<\/jats:italic> kinase mutations were found in 2.6% of patients with chronic\u2010phase chronic myelogenous leukemia (<jats:styled-content style=\"fixed-case\">CML<\/jats:styled-content>), 25.0% of accelerated\u2010phase <jats:styled-content style=\"fixed-case\">CML<\/jats:styled-content>, 66.7% of blast\u2010phase <jats:styled-content style=\"fixed-case\">CML<\/jats:styled-content>, and in 58.3% with Ph+ acute lymphoblastic leukemia. Twelve mutations were identified: 7 T315I, 2 E255V, 1 E255K, 1 F359V, and 1 Y253H. The majority of mutation\u2010positive patients showed an unfavorable clinical course and often had an extra Ph or additional chromosomal abnormalities. Mutations were detected in two patients who had very low or absent <jats:italic><jats:styled-content style=\"fixed-case\">BCR<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">ABL<\/jats:styled-content>1<\/jats:italic> normalized ratios.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Mutation analysis should be performed in Ph+ patients exhibiting an <jats:styled-content style=\"fixed-case\">IMR<\/jats:styled-content> to imatinib. <jats:styled-content style=\"fixed-case\">RFMP<\/jats:styled-content> analysis is helpful for revising therapeutic strategies because it can sensitively detect clinically relevant <jats:italic><jats:styled-content style=\"fixed-case\">ABL<\/jats:styled-content>1<\/jats:italic> kinase mutations with high frequencies.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijlh.12091","type":"journal-article","created":{"date-parts":[[2013,4,10]],"date-time":"2013-04-10T11:16:28Z","timestamp":1365592588000},"page":"589-600","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Detection of <i><scp>ABL<\/scp>1<\/i> kinase mutations in Philadelphia\u2010positive patients exhibiting an inadequate molecular response using restriction fragment mass polymorphism and its clinical significance: a single\u2010center experience in Korea"],"prefix":"10.1111","volume":"35","author":[{"given":"Y.\u2010U.","family":"Cho","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine University of Ulsan College of Medicine and Asan Medical Center Seoul South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.\u2010O.","family":"Kim","sequence":"additional","affiliation":[{"name":"GeneMatrix Inc. Yongin Gyeonggi\u2010do South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.\u2010S.","family":"Chi","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine University of Ulsan College of Medicine and Asan Medical Center Seoul South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.\u2010J.","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine University of Ulsan College of Medicine and Asan Medical Center Seoul South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Jang","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine University of Ulsan College of Medicine and Asan Medical Center Seoul South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.\u2010J.","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine University of Ulsan College of Medicine and Asan Medical Center Seoul South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.\u2010J.","family":"Seo","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine University of Ulsan College of Medicine and Asan Medical Center Seoul South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.\u2010H.","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine University of Ulsan College of Medicine and Asan Medical Center Seoul South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.\u2010H.","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine University of Ulsan College of Medicine and Asan Medical Center Seoul South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.\u2010H.","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine University of Ulsan College of Medicine and Asan Medical Center Seoul South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.\u2010J.","family":"Im","sequence":"additional","affiliation":[{"name":"Department of Pediatrics University of Ulsan College of Medicine and Asan Medical Center Seoul South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.\u2010J.","family":"Seo","sequence":"additional","affiliation":[{"name":"Department of Pediatrics University of Ulsan College of Medicine and Asan Medical Center Seoul South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. 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