{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,21]],"date-time":"2025-11-21T23:00:09Z","timestamp":1763766009538},"reference-count":20,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2013,5,22]],"date-time":"2013-05-22T00:00:00Z","timestamp":1369180800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T00:00:00Z","timestamp":1441065600000},"content-version":"tdm","delay-in-days":832,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2013,12]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>The aim of this study was to characterize clinicopathological features of acute panmyelosis with myelofibrosis (<jats:styled-content style=\"fixed-case\">APMF<\/jats:styled-content>), acute megakaryoblastic leukemia with myelofibrosis (<jats:styled-content style=\"fixed-case\">AMKL<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">MF<\/jats:styled-content>), primary myelofibrosis (<jats:styled-content style=\"fixed-case\">PMF<\/jats:styled-content>) and myelodysplastic syndrome with myelofibrosis (<jats:styled-content style=\"fixed-case\">MDS<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">MF<\/jats:styled-content>) in order to provide the keys to the differential diagnosis of bone marrow (<jats:styled-content style=\"fixed-case\">BM<\/jats:styled-content>) fibrosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We compared age, gender, splenomegaly, serum lactate dehydrogenase level, blood cell counts, blast counts in peripheral blood (<jats:styled-content style=\"fixed-case\">PB<\/jats:styled-content>) and <jats:styled-content style=\"fixed-case\">BM<\/jats:styled-content>, megakaryocyte counts, <jats:styled-content style=\"fixed-case\">BM<\/jats:styled-content> cellularity, dysplasia, and the karyotypes of patients with <jats:styled-content style=\"fixed-case\">APMF<\/jats:styled-content> (<jats:italic>n<\/jats:italic>\u00a0=\u00a06), <jats:styled-content style=\"fixed-case\">AMKL<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">MF<\/jats:styled-content> (<jats:italic>n<\/jats:italic>\u00a0=\u00a07), <jats:styled-content style=\"fixed-case\">PMF<\/jats:styled-content> (<jats:italic>n<\/jats:italic>\u00a0=\u00a044), and <jats:styled-content style=\"fixed-case\">MDS<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">MF<\/jats:styled-content> (<jats:italic>n<\/jats:italic>\u00a0=\u00a044).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">APMF<\/jats:styled-content> showed hyperplasia of all three lineages, increase in megakaryocyte count with dysplasia and frequent abnormal karyotypes. <jats:styled-content style=\"fixed-case\">AMKL<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">MF<\/jats:styled-content> was associated with elevated <jats:styled-content style=\"fixed-case\">BM<\/jats:styled-content> blast counts, decreased <jats:styled-content style=\"fixed-case\">BM<\/jats:styled-content> megakaryocyte count with rare megakaryocytic dysplasia and chromosome 21 abnormality. <jats:styled-content style=\"fixed-case\">PMF<\/jats:styled-content> patients displayed splenomegaly, rare blasts in <jats:styled-content style=\"fixed-case\">PB<\/jats:styled-content>\/<jats:styled-content style=\"fixed-case\">BM<\/jats:styled-content>, and <jats:italic>JAK2 V617F<\/jats:italic> mutation. <jats:styled-content style=\"fixed-case\">MDS<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">MF<\/jats:styled-content> patients showed pancytopenia, dysplasia in all three lineages and recurrent chromosomal abnormalities involving chromosome 5,7,12, and 17.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Although differential diagnosis among <jats:styled-content style=\"fixed-case\">APMF<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> AMKL<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">MF<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> PMF<\/jats:styled-content>, and <jats:styled-content style=\"fixed-case\">MDS<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">MF<\/jats:styled-content> is very challenging due to the overlapping clinical and morphological features, meticulous investigation of the patient with respect to splenomegaly, blood cell count, <jats:styled-content style=\"fixed-case\">PB<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">BM<\/jats:styled-content> findings, and karyotype will serve as a guide to correct diagnosis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijlh.12101","type":"journal-article","created":{"date-parts":[[2013,5,22]],"date-time":"2013-05-22T08:26:57Z","timestamp":1369211217000},"page":"629-636","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Differential diagnosis of myelofibrosis based on <scp>WHO<\/scp> 2008 criteria: Acute panmyelosis with myelofibrosis, acute megakaryoblastic leukemia with myelofibrosis, primary myelofibrosis and myelodysplastic syndrome with myelofibrosis"],"prefix":"10.1111","volume":"35","author":[{"given":"E.","family":"Bae","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine VHS Medical Center Seoul South Korea"},{"name":"Department of Laboratory Medicine College of Medicine and Asan Medical Center University of Ulsan Seoul South 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