{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T11:39:25Z","timestamp":1785929965413,"version":"3.56.0"},"reference-count":12,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2013,7,25]],"date-time":"2013-07-25T00:00:00Z","timestamp":1374710400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T00:00:00Z","timestamp":1441065600000},"content-version":"tdm","delay-in-days":768,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2014,2]]},"abstract":"Summary<\/jats:title>Introduction<\/jats:title>H<\/jats:styled-content>b H<\/jats:styled-content>ofu (HBB<\/jats:styled-content>:c. 380T>A) is a rare inherited hemoglobin abnormality with few case reports in the world literature.<\/jats:p><\/jats:sec>Methods<\/jats:title>Screening for the sickle cell gene mutation and other hemoglobinopathies was carried out using the sickle slide test, H<\/jats:styled-content>b electrophoresis, and HPLC<\/jats:styled-content> under an ongoing central government project.<\/jats:p><\/jats:sec>Results<\/jats:title>We detected twelve Hb Hofu heterozygotes and three sickle H<\/jats:styled-content>b H<\/jats:styled-content>ofu compound heterozygotes. The heterozygotes were asymptomatic except for one individual who had chronic kidney disease and moderate anemia. Only one H<\/jats:styled-content>bS<\/jats:styled-content>\u2010H<\/jats:styled-content>ofu case was symptomatic and presented with intermittent attacks of painful crisis. In the carrier state, the H<\/jats:styled-content>b H<\/jats:styled-content>ofu eluted as a hump at the beginning of the H<\/jats:styled-content>bA<\/jats:styled-content>0<\/jats:sub> window. But in H<\/jats:styled-content>bS<\/jats:styled-content>\u2010H<\/jats:styled-content>ofu cases, H<\/jats:styled-content>b H<\/jats:styled-content>ofu eluted as a single peak in the H<\/jats:styled-content>bA<\/jats:styled-content>0<\/jats:sub> window, with the H<\/jats:styled-content>bA<\/jats:styled-content>2<\/jats:sub> levels being >4% consistently.<\/jats:p><\/jats:sec>Conclusion<\/jats:title>H<\/jats:styled-content>bS<\/jats:styled-content>\u2010H<\/jats:styled-content>ofu has a variable clinical presentation. The retention time of H<\/jats:styled-content>b H<\/jats:styled-content>ofu on HPLC<\/jats:styled-content> is very close to that of H<\/jats:styled-content>bA<\/jats:styled-content>0<\/jats:sub> and often elutes in the A<\/jats:styled-content>0<\/jats:sub> window. Thus, there is every possibility of the H<\/jats:styled-content>bS<\/jats:styled-content>\u2010H<\/jats:styled-content>ofu chromatogram to be misinterpreted as that of a sickle cell trait\/transfused sickle cell\u2010beta\u2010thalassemia case. This is the first time where H<\/jats:styled-content>b H<\/jats:styled-content>ofu has been detected by HPLC<\/jats:styled-content>, which is the widely accepted screening technique for hemoglobinopathies around the world.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijlh.12128","type":"journal-article","created":{"date-parts":[[2013,7,25]],"date-time":"2013-07-25T18:32:29Z","timestamp":1374777149000},"page":"71-76","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Clinical and molecular characterization of H<\/scp>b H<\/scp>ofu in eastern I<\/scp>ndia"],"prefix":"10.1111","volume":"36","author":[{"given":"P.","family":"Purohit","sequence":"first","affiliation":[{"name":"Veer Surendra Sai Medical College Burla Sambalpur Odisha India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. S.","family":"Mashon","sequence":"additional","affiliation":[{"name":"Veer Surendra Sai Medical College Burla Sambalpur Odisha India"},{"name":"Indian Council for Medical Research New Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Patel","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Veer Surendra Sai Medical College Burla Sambalpur Odisha"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Dehury","sequence":"additional","affiliation":[{"name":"Veer Surendra Sai Medical College Burla Sambalpur Odisha India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Pattanayak","sequence":"additional","affiliation":[{"name":"Odisha Sickle cell Project Veer Surendra Sai Medical College Burla Sambalpur Odisha India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Das","sequence":"additional","affiliation":[{"name":"Odisha Sickle cell Project Veer Surendra Sai Medical College Burla Sambalpur Odisha India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Nair","sequence":"additional","affiliation":[{"name":"Department of Haematogenetics National Institute of Immunohaematology(ICMR) KEM Hospital Campus Parel Mumbai Maharashtra India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Italia","sequence":"additional","affiliation":[{"name":"Department of Haematogenetics National Institute of Immunohaematology(ICMR) KEM Hospital Campus Parel Mumbai Maharashtra India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Bag","sequence":"additional","affiliation":[{"name":"Department of Medicine M.K.C.G. Medical College Berhampur Odisha India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Colah","sequence":"additional","affiliation":[{"name":"Department of Haematogenetics National Institute of Immunohaematology(ICMR) KEM Hospital Campus Parel Mumbai Maharashtra India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D. K.","family":"Patel","sequence":"additional","affiliation":[{"name":"Odisha Sickle cell Project Veer Surendra Sai Medical College Burla Sambalpur Odisha India"},{"name":"Department of Medicine Veer Surendra Sai Medical College Burla Sambalpur Odisha India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,7,25]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/217089a0"},{"key":"e_1_2_7_3_1","first-page":"527","article-title":"Detection of abnormal hemoglobin substitutions by a new method application to hb saki and to new cases of HbS hofu, ouled rabah and d. iran","volume":"15","author":"Braconnier F","year":"1975","journal-title":"Nouv Rev Fr Hematol"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.3109\/03630267809005355"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.3109\/03630267909069157"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.3109\/03630269509005819"},{"key":"e_1_2_7_7_1","unstructured":"PatelDK.Clinical aspects of sickle cell disease in India. 5th Brazilian Symposium for Sickle cell disease and other hemoglobinopathies.Belo Horizonte Brazil;2009. 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