{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T07:38:12Z","timestamp":1791617892726,"version":"4.3.4"},"reference-count":25,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2013,9,3]],"date-time":"2013-09-03T00:00:00Z","timestamp":1378166400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T00:00:00Z","timestamp":1441065600000},"content-version":"tdm","delay-in-days":728,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2014,2]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>\n                      Patients with persistent high levels of serum vitamin\n                      <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>\n                      12 were often referred to Hematology departments. In this study, characteristic of patients with serum vitamin\n                      <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>\n                      12 levels higher than 2500\u00a0pmol\/L (high\n                      <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>\n                      12) were studied.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Prevalence of high\n                      <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>\n                      12 was evaluated during a 10\u2010month period. Samples with high\n                      <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>\n                      12 were incubated with polyethylene glycol (\n                      <jats:styled-content style=\"fixed-case\">PEG<\/jats:styled-content>\n                      ) and a new measurement of vitamin\n                      <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>\n                      12 was carried out using the supernatant. As a pilot study, 26 frozen samples with high\n                      <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>\n                      12 were evaluated for changes in vitamin\n                      <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>\n                      12 after\n                      <jats:styled-content style=\"fixed-case\">PEG<\/jats:styled-content>\n                      . Moreover, a prospective study was carried out during three consecutive months. Size exclusion chromatography was employed to demonstrate the presence of immune complexes (\n                      <jats:styled-content style=\"fixed-case\">IC<\/jats:styled-content>\n                      s) with plasma vitamin\n                      <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>\n                      12\u2010binding proteins in some serum samples with high\n                      <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>\n                      12.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Prevalence of high\n                      <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>\n                      12 was 1.3%. Results from 26 frozen samples and from a prospective study (28 cases) showed that undergoing vitamin\n                      <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>\n                      12 treatment was the main cause of high\n                      <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>\n                      12. However,\n                      <jats:styled-content style=\"fixed-case\">IC<\/jats:styled-content>\n                      s were detected in 10 frozen samples and in seven cases (25%) of the prospective study, respectively. Serum vitamin\n                      <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>\n                      12 decreased to normal values after precipitation with\n                      <jats:styled-content style=\"fixed-case\">PEG<\/jats:styled-content>\n                      , and size exclusion chromatography confirmed\n                      <jats:styled-content style=\"fixed-case\">IC<\/jats:styled-content>\n                      s. An association with autoimmune or hematological disorders was observed.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      In patients with repeatedly high\n                      <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>\n                      12 levels,\n                      <jats:styled-content style=\"fixed-case\">IC<\/jats:styled-content>\n                      s were detected in approximately 25% of samples. Precipitation with\n                      <jats:styled-content style=\"fixed-case\">PEG<\/jats:styled-content>\n                      is an easy method to confirm the presence of\n                      <jats:styled-content style=\"fixed-case\">IC<\/jats:styled-content>\n                      s and to evaluate serum vitamin\n                      <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>\n                      12 levels in these patients.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/ijlh.12131","type":"journal-article","created":{"date-parts":[[2013,9,3]],"date-time":"2013-09-03T03:05:28Z","timestamp":1378177528000},"page":"92-97","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":36,"title":["Immune complexes and persistent high levels of serum vitamin\n                    <scp>B<\/scp>\n                    12"],"prefix":"10.1111","volume":"36","author":[{"given":"A. 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