{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T09:03:19Z","timestamp":1762506199802},"reference-count":36,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2013,11,5]],"date-time":"2013-11-05T00:00:00Z","timestamp":1383609600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T00:00:00Z","timestamp":1441065600000},"content-version":"tdm","delay-in-days":665,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2014,4]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>Paroxysmal nocturnal hemoglobinuria (<jats:styled-content style=\"fixed-case\">PNH<\/jats:styled-content>) is a hemolytic, clonal and acquired disorder of the hematopoietic stem cell with a deficiency of all glycophosphatidyl\u2010inositol (<jats:styled-content style=\"fixed-case\">GPI<\/jats:styled-content>) linked proteins. The aim of this retrospective study was to analyse haematological and biochemical data from 152 patients referred to our laboratory for diagnosis of<jats:styled-content style=\"fixed-case\">PNH<\/jats:styled-content>by flow cytometry (<jats:styled-content style=\"fixed-case\">FC<\/jats:styled-content>).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Patients and healthy donor (152 and 99 respectively) were studied. Ham, sucrose, lactate dehydrogenase (<jats:styled-content style=\"fixed-case\">LDH<\/jats:styled-content>), Iron, haptoglobin (Hp), blood cell morphology and Kaplow cytochemical stain for leukocyte alkaline phosphatase (<jats:styled-content style=\"fixed-case\">LAP<\/jats:styled-content>) were carried out.<jats:styled-content style=\"fixed-case\">GPI<\/jats:styled-content>\u2010proteins anti\u2010<jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>55 and<jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>59 in erythrocytes and the former, plus anti<jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>16b and<jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>66b on neutrophils were evaluated by<jats:styled-content style=\"fixed-case\">FC<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Anemia and\/or leukopenia and\/or thrombocytopenia, increased reticulocyte count and<jats:styled-content style=\"fixed-case\">LDH<\/jats:styled-content>were observed in patients with<jats:styled-content style=\"fixed-case\">PNH<\/jats:styled-content>clone. Some of them had dacriocytes, schistocytes.<jats:styled-content style=\"fixed-case\">LAP<\/jats:styled-content>was low. On average, we detected 50%<jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>59 (\u2212) erythrocytes and 29, 83, 78%<jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>55\/59 (\u2212),<jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>16b (\u2212),<jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>66b (\u2212) neutrophils, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Paroxysmal nocturnal hemoglobinuria clone was detected in 20\/152 patients. Negative population's percentages were high in patients with classic<jats:styled-content style=\"fixed-case\">PNH<\/jats:styled-content>, Hematimetry,<jats:styled-content style=\"fixed-case\">LAP<\/jats:styled-content>and adequate use of<jats:styled-content style=\"fixed-case\">CF<\/jats:styled-content>contribute to<jats:styled-content style=\"fixed-case\">PNH<\/jats:styled-content>clone detection in the laboratory.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijlh.12156","type":"journal-article","created":{"date-parts":[[2013,11,5]],"date-time":"2013-11-05T09:11:39Z","timestamp":1383642699000},"page":"213-221","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Paroxysmal nocturnal haemoglobinuria. 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