{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,9,25]],"date-time":"2023-09-25T05:11:27Z","timestamp":1695618687966},"reference-count":19,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2014,6,2]],"date-time":"2014-06-02T00:00:00Z","timestamp":1401667200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T00:00:00Z","timestamp":1441065600000},"content-version":"tdm","delay-in-days":456,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2014,12]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>B\u2010chronic lymphocytic leukemia <jats:styled-content style=\"fixed-case\">CLL<\/jats:styled-content>, a neoplastic clonal disorder with monomorphous small B lymphocytes with scanty cytoplasm and clumped chromatin, can be morphologically differentiated in typical and atypical forms with different prognosis: Smudge cells (Gumprecht's shadows) are one of the well\u2010known features of the typical <jats:styled-content style=\"fixed-case\">CLL<\/jats:styled-content> and are much less inconsistent in other different types <jats:styled-content style=\"fixed-case\">CLPD<\/jats:styled-content>. Abbott Cell\u2010Dyn Sapphire uses the fluorescence after staining with the <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> fluorochrome propidium iodide for the measurement of nucleated red blood cells (<jats:styled-content style=\"fixed-case\">NRBC<\/jats:styled-content>s) and nonviable cells (<jats:styled-content style=\"fixed-case\">FL<\/jats:styled-content>3+ cell fraction): We have studied the possible correlation between presence and number of morphologically identifiable smudge cells on smears and the percentage of nonviable cells produced by Cell\u2010Dyn Sapphire.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>305 blood samples from 224 patients with B\u2010cell lymphoproliferative disorders and 40 healthy blood donors were analyzed by <jats:styled-content style=\"fixed-case\">CBC<\/jats:styled-content> performed by Cell\u2010Dyn Sapphire, peripheral blood smear, and immunophenotype characterization.<\/jats:p><\/jats:sec><jats:sec><jats:title>Result<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">FL<\/jats:styled-content>3+ fraction in <jats:styled-content style=\"fixed-case\">CLPD<\/jats:styled-content> directly correlated with the percentage of smudge cells and is significantly increased in patients with typical B\u2010<jats:styled-content style=\"fixed-case\">CLL<\/jats:styled-content>. This phenomenon is much less evident in patients with atypical\/mixed B\u2010<jats:styled-content style=\"fixed-case\">CLL<\/jats:styled-content> and B\u2010<jats:styled-content style=\"fixed-case\">NHL<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>In small laboratories without <jats:styled-content style=\"fixed-case\">FCM<\/jats:styled-content> and cytogenetic, smudge cells%, can be utilized as a preliminary diagnostic and prognostic tool in differential diagnosis of <jats:styled-content style=\"fixed-case\">CLPD<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijlh.12198","type":"journal-article","created":{"date-parts":[[2014,6,2]],"date-time":"2014-06-02T17:44:30Z","timestamp":1401731070000},"page":"628-635","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Automated quantification of apoptosis in B\u2010cell chronic lymphoproliferative disorders: a prognostic variable obtained with the Cell\u2010Dyn Sapphire (Abbott) automated hematology analyzer"],"prefix":"10.1111","volume":"36","author":[{"given":"M.","family":"Fumi","sequence":"first","affiliation":[{"name":"Clinical Pathology Laboratory A.O.R.N \u2018G. 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