{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,6]],"date-time":"2025-10-06T09:28:09Z","timestamp":1759742889830},"reference-count":13,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2014,4,4]],"date-time":"2014-04-04T00:00:00Z","timestamp":1396569600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T00:00:00Z","timestamp":1441065600000},"content-version":"tdm","delay-in-days":515,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2015,2]]},"abstract":"Summary<\/jats:title>Introduction<\/jats:title>Flow cytometry is the most sensitive and specific diagnostic modality for the assessment of clone size in paroxysmal nocturnal haemoglobinuria (PNH<\/jats:styled-content>) and other bone marrow failure states. In this study, we attempt to distinguish PNH<\/jats:styled-content> from aplastic anaemia (AA<\/jats:styled-content>) and myelodysplastic syndromes (MDS<\/jats:styled-content>) associated with PNH<\/jats:styled-content> clones at diagnosis by clone size, clinical and laboratory features.<\/jats:p><\/jats:sec>Methods<\/jats:title>A total of 29 samples included 19 PNH<\/jats:styled-content> cases and 10 AA<\/jats:styled-content>\/MDS<\/jats:styled-content> cases with PNH<\/jats:styled-content> clones. Flow cytometry was performed using fluorescent aerolysin (FLAER<\/jats:styled-content>)\u2010based assay and comparison of clinical features, laboratory parameters and PNH<\/jats:styled-content> clone size was carried out at diagnosis.<\/jats:p><\/jats:sec>Results<\/jats:title>The PNH<\/jats:styled-content> clone size on granulocytes varied from 0.4% to 99.2% and correlated with the clone size on monocytes (r<\/jats:italic>\u00a0=\u00a00.966; P<\/jats:italic>\u00a0<\u00a00.001). Paroxysmal nocturnal haemoglobinuria clone size on granulocytes (median\u00a0=\u00a034.6%) and monocytes (median\u00a0=\u00a049.9%) was always larger than erythrocytes (median\u00a0=\u00a010.9%). The median clone size in PNH<\/jats:styled-content> (median granulocytes\u00a0=\u00a074.9%, monocytes\u00a0=\u00a071.8%) was significantly greater than in AA<\/jats:styled-content>\/MDS<\/jats:styled-content> associated with PNH<\/jats:styled-content> clone (median granulocytes\u00a0=\u00a02.9%, monocytes\u00a0=\u00a06%). In PNH<\/jats:styled-content> patients, a significant negative correlation was seen between PNH<\/jats:styled-content> clone on monocytes and the haemoglobin concentration.<\/jats:p><\/jats:sec>Conclusion<\/jats:title>In our small study using the FLAER<\/jats:styled-content> method, the clone size was >70% in majority of PNH<\/jats:styled-content> cases. In other marrow disorders like AA<\/jats:styled-content>\/MDS<\/jats:styled-content>, the clone size was usually <10%.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijlh.12207","type":"journal-article","created":{"date-parts":[[2014,4,4]],"date-time":"2014-04-04T12:00:09Z","timestamp":1396612809000},"page":"14-21","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Analysis of abnormal clones by the fluorescent aerolysin method in paroxysmal nocturnal haemoglobinuria and other marrow disorders"],"prefix":"10.1111","volume":"37","author":[{"given":"R.","family":"Agarwal","sequence":"first","affiliation":[{"name":"Department of Diagnostic Royal Melbourne Hospital Parkville Vic. Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"Chapple","sequence":"additional","affiliation":[{"name":"Department of Diagnostic Royal Melbourne Hospital Parkville Vic. 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