{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T16:43:37Z","timestamp":1773679417367,"version":"3.50.1"},"reference-count":12,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2014,3,24]],"date-time":"2014-03-24T00:00:00Z","timestamp":1395619200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T00:00:00Z","timestamp":1441065600000},"content-version":"tdm","delay-in-days":526,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2015,2]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>Osteolytic bone destruction is a major clinical problem in multiple myeloma patients. Osteoclasts can differentiate <jats:italic>in vitro<\/jats:italic> from bone marrow\u2010resident monocyte progenitors, such as common monocyte progenitors, as well as circulating monocytes. Various types of monocytes, including osteoclast precursors, appear to circulate systemically.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We investigated the possibility of demonstrating, by <jats:italic>in vitro<\/jats:italic> differentiation and flow cytometry, a circulating osteoclast precursor population in multiple myeloma (MM) patients by studying the distribution of <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>14<jats:sup>+\/++<\/jats:sup> <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>11b<jats:sup>+<\/jats:sup> <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>51\/61<jats:sup>+<\/jats:sup> and <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>14<jats:sup>+\/++<\/jats:sup> <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>16<jats:sup>+\/\u2212<\/jats:sup> populations.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Under short\u2010term <jats:italic>in vitro<\/jats:italic> osteoclastic differentiation conditions, almost all <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>14 monocytes acquired <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>51\/61 and <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>16 expression. Flow cytometry studies failed to demonstrate a statistically significant increase in circulating <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>14<jats:sup>+\/++<\/jats:sup> <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>11b<jats:sup>+<\/jats:sup> <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>51\/61<jats:sup>+<\/jats:sup> populations in 20 MM patients with osteolytic lesions. However, the minor circulating <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>14<jats:sup>+\/++<\/jats:sup> <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>16<jats:sup>+<\/jats:sup> fraction was significantly increased in <jats:styled-content style=\"fixed-case\">MM<\/jats:styled-content> patients compared with healthy volunteers (109.3\u00a0\u00b1\u00a063.1\/mm<jats:sup>3<\/jats:sup> <jats:italic>vs<\/jats:italic>. 65.3\u00a0\u00b1\u00a034.9\/mm<jats:sup>3<\/jats:sup>; <jats:italic>P<\/jats:italic>\u00a0=\u00a00.005), but with no correlation with markers of tumour burden. The <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>14<jats:sup>+\/++<\/jats:sup> <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>16<jats:sup>+<\/jats:sup> to <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>14<jats:sup>+\/++<\/jats:sup> <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>16<jats:sup>\u2212<\/jats:sup> ratio was higher in <jats:styled-content style=\"fixed-case\">MM<\/jats:styled-content> patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The circulating <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>14<jats:sup>+\/++<\/jats:sup> <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>11b<jats:sup>+<\/jats:sup> <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>51\/61<jats:sup>+<\/jats:sup> fraction was not correlated with bone lesions in <jats:styled-content style=\"fixed-case\">MM<\/jats:styled-content> patients. However, <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>14<jats:sup>+\/++<\/jats:sup> <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>16<jats:sup>+<\/jats:sup> monocytes may be a candidate marker. A larger study must be conducted to confirm these promising results for the diagnosis and follow\u2010up of <jats:styled-content style=\"fixed-case\">MM<\/jats:styled-content> patients.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijlh.12216","type":"journal-article","created":{"date-parts":[[2014,3,25]],"date-time":"2014-03-25T02:00:30Z","timestamp":1395712830000},"page":"29-35","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["<scp>CD<\/scp>14<sup>+<\/sup><scp>CD<\/scp>16<sup>+<\/sup> monocytes rather than <scp>CD<\/scp>14<sup>+<\/sup><scp>CD<\/scp>51\/61<sup>+<\/sup> monocytes are a potential cytological marker of circulating osteoclast precursors in multiple myeloma. A preliminary study"],"prefix":"10.1111","volume":"37","author":[{"given":"V.","family":"Petitprez","sequence":"first","affiliation":[{"name":"Haematology and Histocompatibility Laboratory Amiens University Hospital Amiens France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B.","family":"Royer","sequence":"additional","affiliation":[{"name":"Department of Clinical Haematology and Cell Therapy Amiens University Hospital Amiens France"},{"name":"EA4666 \u2018Lymphocyte Normal Pathologique et Cancer\u2019 University of Picardie Amiens France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Desoutter","sequence":"additional","affiliation":[{"name":"Haematology and Histocompatibility Laboratory Amiens University Hospital Amiens France"},{"name":"INSERM UMR\u2010S 1088 University of Picardie Amiens France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.","family":"Guiheneuf","sequence":"additional","affiliation":[{"name":"Haematology and Histocompatibility Laboratory Amiens University Hospital Amiens France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Rigolle","sequence":"additional","affiliation":[{"name":"Haematology and Histocompatibility Laboratory Amiens University Hospital Amiens France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. P.","family":"Marolleau","sequence":"additional","affiliation":[{"name":"Department of Clinical Haematology and Cell Therapy Amiens University Hospital Amiens France"},{"name":"EA4666 \u2018Lymphocyte Normal Pathologique et Cancer\u2019 University of Picardie Amiens France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Kamel","sequence":"additional","affiliation":[{"name":"INSERM UMR\u2010S 1088 University of Picardie Amiens France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N.","family":"Guillaume","sequence":"additional","affiliation":[{"name":"Haematology and Histocompatibility Laboratory Amiens University Hospital Amiens France"},{"name":"EA4666 \u2018Lymphocyte Normal Pathologique et Cancer\u2019 University of Picardie Amiens France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,3,24]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.leukres.2006.04.014"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejca.2005.11.034"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/rheumatology\/kes259"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2141.1999.01491.x"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2000.02379.x"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12026-012-8297-3"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.04-2214fje"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/ar2915"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2005.09.146"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1359\/jbmr.071031"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00223-012-9654-x"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.leukres.2008.07.028"}],"container-title":["International Journal of Laboratory Hematology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fijlh.12216","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ijlh.12216","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,24]],"date-time":"2023-09-24T15:02:58Z","timestamp":1695567778000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/ijlh.12216"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,3,24]]},"references-count":12,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2015,2]]}},"alternative-id":["10.1111\/ijlh.12216"],"URL":"https:\/\/doi.org\/10.1111\/ijlh.12216","archive":["Portico"],"relation":{},"ISSN":["1751-5521","1751-553X"],"issn-type":[{"value":"1751-5521","type":"print"},{"value":"1751-553X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,3,24]]},"assertion":[{"value":"2014-04-23","name":"published_online","label":"Published Online","group":{"name":"publication_history","label":"Publication History"}}]}}