{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T09:19:57Z","timestamp":1774430397607,"version":"3.50.1"},"reference-count":36,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2014,4,18]],"date-time":"2014-04-18T00:00:00Z","timestamp":1397779200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T00:00:00Z","timestamp":1441065600000},"content-version":"tdm","delay-in-days":501,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2014,6]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>The development of an automated, von Willebrand factor (<jats:styled-content style=\"fixed-case\">VWF<\/jats:styled-content>) activity assay, Innovance<jats:sup>\u00ae<\/jats:sup> <jats:styled-content style=\"fixed-case\">VWF<\/jats:styled-content> Ac (<jats:styled-content style=\"fixed-case\">VWF<\/jats:styled-content>:Ac), which measures <jats:styled-content style=\"fixed-case\">VWF<\/jats:styled-content> binding to the platelet receptor glycoprotein Ib\u03b1 without ristocetin, led us to evaluate the assay for diagnosing von Willebrand disease (<jats:styled-content style=\"fixed-case\">VWD<\/jats:styled-content>) and monitoring therapy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>After validating that the assay could be performed on an instrument from a different manufacturer, we compared <jats:styled-content style=\"fixed-case\">VWF<\/jats:styled-content>:Ac to <jats:styled-content style=\"fixed-case\">VWF<\/jats:styled-content> ristocetin cofactor activity (<jats:styled-content style=\"fixed-case\">VWF<\/jats:styled-content>:<jats:styled-content style=\"fixed-case\">RC<\/jats:styled-content>o) findings, including ratios of activity\/antigen, for 100 healthy controls and 262 consecutive clinical samples from 217 patients (197 adults, 64 children, <jats:italic>n<\/jats:italic>\u00a0=\u00a01 age unknown) referred for <jats:styled-content style=\"fixed-case\">VWF<\/jats:styled-content> testing.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>There was excellent correlation (<jats:italic>R<\/jats:italic><jats:sup>2<\/jats:sup>\u00a0=\u00a00.96) between <jats:styled-content style=\"fixed-case\">VWF<\/jats:styled-content>:Ac results run at two different sites on two different instruments. <jats:styled-content style=\"fixed-case\">VWF<\/jats:styled-content>:Ac had greater precision and sensitivity to low levels of <jats:styled-content style=\"fixed-case\">VWF<\/jats:styled-content> than the <jats:styled-content style=\"fixed-case\">VWF<\/jats:styled-content>:<jats:styled-content style=\"fixed-case\">RC<\/jats:styled-content>o method. Although there was good correlation between <jats:styled-content style=\"fixed-case\">VWF<\/jats:styled-content>:Ac and <jats:styled-content style=\"fixed-case\">VWF<\/jats:styled-content>:<jats:styled-content style=\"fixed-case\">RC<\/jats:styled-content>o results among healthy controls and patient subjects, <jats:styled-content style=\"fixed-case\">VWF<\/jats:styled-content>:Ac results were undetectable and\/or significantly lower than <jats:styled-content style=\"fixed-case\">VWF<\/jats:styled-content>:<jats:styled-content style=\"fixed-case\">RC<\/jats:styled-content>o among patients who had types 2A, 2B, or 2M <jats:styled-content style=\"fixed-case\">VWD<\/jats:styled-content>. Additionally, a higher proportion of patient samples were classified as showing qualitative defects using the <jats:styled-content style=\"fixed-case\">VWF<\/jats:styled-content>:Ac compared with <jats:styled-content style=\"fixed-case\">VWF<\/jats:styled-content>:<jats:styled-content style=\"fixed-case\">RC<\/jats:styled-content>o method. While most samples drawn on <jats:styled-content style=\"fixed-case\">VWD<\/jats:styled-content> therapy had similar <jats:styled-content style=\"fixed-case\">VWF<\/jats:styled-content> levels by <jats:styled-content style=\"fixed-case\">VWF<\/jats:styled-content>:Ac and <jats:styled-content style=\"fixed-case\">VWF<\/jats:styled-content>:<jats:styled-content style=\"fixed-case\">RC<\/jats:styled-content>o, a type 2B <jats:styled-content style=\"fixed-case\">VWD<\/jats:styled-content> subject on replacement had much lower activity estimated by <jats:styled-content style=\"fixed-case\">VWF<\/jats:styled-content>:Ac.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>We conclude that Innovance<jats:sup>\u00ae<\/jats:sup> <jats:styled-content style=\"fixed-case\">VWF<\/jats:styled-content> Ac is suitable for the diagnosis, classification, and monitoring of <jats:styled-content style=\"fixed-case\">VWD<\/jats:styled-content>, and that it has a number of advantages over <jats:styled-content style=\"fixed-case\">VWF<\/jats:styled-content>:<jats:styled-content style=\"fixed-case\">RC<\/jats:styled-content>o method.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijlh.12218","type":"journal-article","created":{"date-parts":[[2014,4,19]],"date-time":"2014-04-19T09:38:30Z","timestamp":1397900310000},"page":"341-351","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":44,"title":["Evaluation of an automated method for measuring von <scp>W<\/scp>illebrand factor activity in clinical samples without ristocetin"],"prefix":"10.1111","volume":"36","author":[{"given":"L.","family":"Graf","sequence":"first","affiliation":[{"name":"Department of Pathology and Molecular Medicine McMaster University Hamilton Ontario Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K. 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