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The presence of a genetic defect in one of the major contributing components (protein <jats:styled-content style=\"fixed-case\">C<\/jats:styled-content> [<jats:styled-content style=\"fixed-case\">PC<\/jats:styled-content>], protein <jats:styled-content style=\"fixed-case\">S<\/jats:styled-content> [<jats:styled-content style=\"fixed-case\">PS<\/jats:styled-content>], and antithrombin [<jats:styled-content style=\"fixed-case\">AT<\/jats:styled-content>]) to thrombophilia can be determined by clinical laboratory assays. However, understanding the limitations and problems associated with assays is paramount to an accurate analysis of the genetic status. This review will discuss the major analytical issues and provide recommendations for assaying <jats:styled-content style=\"fixed-case\">PC<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> PS<\/jats:styled-content>, and <jats:styled-content style=\"fixed-case\">AT<\/jats:styled-content> in plasma. Recommendations are also made about pre\u2010analytical and postanalytical issues clinically affecting these assays.<\/jats:p>","DOI":"10.1111\/ijlh.12219","type":"journal-article","created":{"date-parts":[[2014,4,19]],"date-time":"2014-04-19T09:38:30Z","timestamp":1397900310000},"page":"289-295","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":59,"title":["Laboratory testing issues for protein C, protein S, and antithrombin"],"prefix":"10.1111","volume":"36","author":[{"given":"R. A.","family":"Marlar","sequence":"first","affiliation":[{"name":"Pathology and Laboratory Medicine Services Oklahoma City VA Medical Center Oklahoma City OK USA"},{"name":"Department of Pathology University of Oklahoma Health Sciences Center Oklahoma City OK USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. 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