{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T16:47:10Z","timestamp":1771001230582,"version":"3.50.1"},"reference-count":44,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2014,4,18]],"date-time":"2014-04-18T00:00:00Z","timestamp":1397779200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T00:00:00Z","timestamp":1441065600000},"content-version":"tdm","delay-in-days":501,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2014,6]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Diagnostic tests for von Willebrand disease (<jats:styled-content style=\"fixed-case\">VWD<\/jats:styled-content>) are important for the assessment of <jats:styled-content style=\"fixed-case\">VWD<\/jats:styled-content>, which is a commonly encountered bleeding disorder worldwide. Technical innovations have been applied to improve the precision and lower limit of detection of von Willebrand factor (<jats:styled-content style=\"fixed-case\">VWF<\/jats:styled-content>) assays, including the ristocetin cofactor activity assay (<jats:styled-content style=\"fixed-case\">VWF<\/jats:styled-content>:<jats:styled-content style=\"fixed-case\">RC<\/jats:styled-content>o) that uses the antibiotic ristocetin to induce plasma <jats:styled-content style=\"fixed-case\">VWF<\/jats:styled-content> binding to glycoprotein (<jats:styled-content style=\"fixed-case\">GP<\/jats:styled-content>) Ib<jats:styled-content style=\"fixed-case\">IXV<\/jats:styled-content> on target platelets. <jats:styled-content style=\"fixed-case\">VWF<\/jats:styled-content>\u2010collagen\u2010binding assays, depending on the type of collagen used, can improve the detection of forms of <jats:styled-content style=\"fixed-case\">VWD<\/jats:styled-content> with high molecular weight <jats:styled-content style=\"fixed-case\">VWF<\/jats:styled-content> multimer loss, although the best method is debatable. A number of innovations have been applied to <jats:styled-content style=\"fixed-case\">VWF<\/jats:styled-content>:<jats:styled-content style=\"fixed-case\">RC<\/jats:styled-content>o (which is commonly performed on an aggregometer), including replacing the target platelets with immobilized <jats:styled-content style=\"fixed-case\">GPI<\/jats:styled-content>b\u03b1, and quantification by an enzyme\u2010linked immunosorbent assay (<jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content>), immunoturbidimetric, or chemiluminescent end\u2010point. Some common polymorphisms in the <jats:styled-content style=\"fixed-case\">VWF<\/jats:styled-content> gene that do not cause bleeding are associated with falsely low <jats:styled-content style=\"fixed-case\">VWF<\/jats:styled-content> activity by ristocetin\u2010dependent methods. To overcome the need for ristocetin, some new <jats:styled-content style=\"fixed-case\">VWF<\/jats:styled-content> activity assays use gain\u2010of\u2010function <jats:styled-content style=\"fixed-case\">GPI<\/jats:styled-content>b\u03b1 mutants that bind <jats:styled-content style=\"fixed-case\">VWF<\/jats:styled-content> without the need for ristocetin, with an improved precision and lower limit of detection than measuring <jats:styled-content style=\"fixed-case\">VWF<\/jats:styled-content>:<jats:styled-content style=\"fixed-case\">RC<\/jats:styled-content>o by aggregometry. <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content> of <jats:styled-content style=\"fixed-case\">VWF<\/jats:styled-content> binding to mutated <jats:styled-content style=\"fixed-case\">GPI<\/jats:styled-content>b\u03b1 shows promise as a method to identify gain\u2010of\u2010function defects from type 2B <jats:styled-content style=\"fixed-case\">VWD<\/jats:styled-content>. The performance characteristics of many new <jats:styled-content style=\"fixed-case\">VWF<\/jats:styled-content> activity assays suggest that the detection of <jats:styled-content style=\"fixed-case\">VWD<\/jats:styled-content>, and monitoring of <jats:styled-content style=\"fixed-case\">VWD<\/jats:styled-content> therapy, by clinical laboratories could be improved through adopting newer generation <jats:styled-content style=\"fixed-case\">VWF<\/jats:styled-content> assays.<\/jats:p>","DOI":"10.1111\/ijlh.12220","type":"journal-article","created":{"date-parts":[[2014,4,19]],"date-time":"2014-04-19T09:38:30Z","timestamp":1397900310000},"page":"334-340","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Technological advances in diagnostic testing for von Willebrand disease: new approaches and challenges"],"prefix":"10.1111","volume":"36","author":[{"given":"C. P. 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