{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T03:12:06Z","timestamp":1773025926183,"version":"3.50.1"},"reference-count":26,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2014,4,17]],"date-time":"2014-04-17T00:00:00Z","timestamp":1397692800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T00:00:00Z","timestamp":1441065600000},"content-version":"tdm","delay-in-days":502,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2015,2]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>Multidimensional optical scatter of sphered erythrocytes can identify and enumerate hyperchromic erythrocytes, which might be used for hereditary spherocytosis (<jats:styled-content style=\"fixed-case\">HS<\/jats:styled-content>) screening. The flow cytometric eosin\u20105\u2032\u2010maleimide test (<jats:styled-content style=\"fixed-case\">EMA<\/jats:styled-content>) is highly sensitive and specific for <jats:styled-content style=\"fixed-case\">HS<\/jats:styled-content> as a confirmatory method. The aims of this study were to assess the utility of hyperchromic erythrocytes in <jats:styled-content style=\"fixed-case\">HS<\/jats:styled-content> screening and to evaluate the <jats:styled-content style=\"fixed-case\">EMA<\/jats:styled-content> test performed on <jats:styled-content style=\"fixed-case\">CELL<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">DYN<\/jats:styled-content> Sapphire analyser compared with the reference method.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Blood from 740 paediatric patients presenting at our institution was analysed in reticulocyte mode of the <jats:styled-content style=\"fixed-case\">CELL<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">DYN<\/jats:styled-content> Sapphire haematology analyser (Abbott Diagnostics) to obtain hyperchromic erythrocyte counts. The <jats:styled-content style=\"fixed-case\">EMA<\/jats:styled-content> test was performed using a flow cytometer as a reference, as well as <jats:styled-content style=\"fixed-case\">CELL<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">DYN<\/jats:styled-content> Sapphire as an investigational method.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Hyperchromic erythrocytes were the highest in patients with <jats:styled-content style=\"fixed-case\">HS<\/jats:styled-content> (median 11.5%; range 5.1\u201329.2%). Patients with autoimmune haemolytic disease had significantly less hyperchromic erythrocytes (median 4.9%; range 0.0\u201318.3%). Hyperchromic erythrocytes showed a high area under the <jats:styled-content style=\"fixed-case\">ROC<\/jats:styled-content> curve: 0.972. At 4.9% cut\u2010off, hyperchromic erythrocytes detected <jats:styled-content style=\"fixed-case\">HS<\/jats:styled-content> with 96.4% sensitivity and 99.1% specificity. The <jats:styled-content style=\"fixed-case\">EMA<\/jats:styled-content> test on <jats:styled-content style=\"fixed-case\">CELL<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">DYN<\/jats:styled-content> Sapphire correlated strongly with the reference test and had identical diagnostic power. Stability studies with blood from <jats:styled-content style=\"fixed-case\">HS<\/jats:styled-content> patients showed a significant decrease in hyperchromic erythrocytes after 6\u00a0h storage.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Measurement of hyperchromic erythrocytes is highly sensitive and specific for detecting <jats:styled-content style=\"fixed-case\">HS<\/jats:styled-content> and can be used for rapid and inexpensive screening. If required, the <jats:styled-content style=\"fixed-case\">EMA<\/jats:styled-content> test can be performed on <jats:styled-content style=\"fixed-case\">CELL<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">DYN<\/jats:styled-content> Sapphire or a standard flow cytometer for confirmation of <jats:styled-content style=\"fixed-case\">HS<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijlh.12245","type":"journal-article","created":{"date-parts":[[2014,4,17]],"date-time":"2014-04-17T06:31:06Z","timestamp":1397716266000},"page":"98-104","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Screening and confirmation of hereditary spherocytosis in children using a <scp>CELL<\/scp>\u2010<scp>DYN<\/scp> Sapphire haematology analyser"],"prefix":"10.1111","volume":"37","author":[{"given":"S.","family":"Rooney","sequence":"first","affiliation":[{"name":"Haematology Department Our Lady's Children's Hospital Crumlin Dublin Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. 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M.","family":"Cormack","sequence":"additional","affiliation":[{"name":"Haematology Department Our Lady's Children's Hospital Crumlin Dublin Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"McMahon","sequence":"additional","affiliation":[{"name":"Haematology Department Our Lady's Children's Hospital Crumlin Dublin Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,4,17]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2011.052845"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2008-07-161166"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/ijlh.12070"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(08)61588-3"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2011.08921.x"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.20413"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00277-010-1112-0"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1159\/000322253"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2012.074070"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2007.06944.x"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2012.072660"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1364\/AO.24.001355"},{"key":"e_1_2_7_14_1","first-page":"547","article-title":"Flow cytometric measurement (H\u20101 Technicon) of microcytic and hyperchromic red cell populations in pediatric patients affected by hereditary spherocytosis (HS)","volume":"74","author":"Ialongo P","year":"1989","journal-title":"Haematologica"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2257.1989.tb00171.x"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2257.1996.00157.x"},{"key":"e_1_2_7_17_1","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1111\/j.1365-2257.1996.tb01288.x","article-title":"The effect of splenectomy in hereditary spherocytosis by dual angle laser light cytometry","volume":"18","author":"Pilar Ricard M","year":"1996","journal-title":"Clin Lab Haematol"},{"key":"e_1_2_7_18_1","first-page":"163","article-title":"Demonstration and quantification of hyperchromic erythrocytes by haematological analysers Application to screening for hereditary and acquired spherocytosis","volume":"48","author":"Kutter D","year":"2002","journal-title":"Clin Lab"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-553X.2011.01391.x"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2000.02416.x"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1532\/LH96.04017"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1515\/cclm-2011-0796"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1097\/MOH.0b013e32835f5933"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/100.2.119"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.55.11.841"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2012.075150"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-553X.2008.01098.x"}],"container-title":["International Journal of Laboratory Hematology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fijlh.12245","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ijlh.12245","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,24]],"date-time":"2023-09-24T15:03:51Z","timestamp":1695567831000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/ijlh.12245"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,4,17]]},"references-count":26,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2015,2]]}},"alternative-id":["10.1111\/ijlh.12245"],"URL":"https:\/\/doi.org\/10.1111\/ijlh.12245","archive":["Portico"],"relation":{},"ISSN":["1751-5521","1751-553X"],"issn-type":[{"value":"1751-5521","type":"print"},{"value":"1751-553X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,4,17]]},"assertion":[{"value":"2014-04-23","name":"published_online","label":"Published Online","group":{"name":"publication_history","label":"Publication History"}}]}}