{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T03:46:53Z","timestamp":1781668013757,"version":"3.54.5"},"reference-count":29,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2014,5,28]],"date-time":"2014-05-28T00:00:00Z","timestamp":1401235200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T00:00:00Z","timestamp":1441065600000},"content-version":"tdm","delay-in-days":461,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2015,4]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>The new red blood cell (<jats:styled-content style=\"fixed-case\">RBC<\/jats:styled-content>) parameters such as reticulocyte haemoglobin content and percentage of hypochromic red cells or equivalent, although useful in the laboratory assessment of iron deficiency anaemia (<jats:styled-content style=\"fixed-case\">IDA<\/jats:styled-content>), are confounded by thalassaemia trait (<jats:styled-content style=\"fixed-case\">TT<\/jats:styled-content>). We aim to evaluate the new red cell parameters on the Beckman Coulter DxH800 in distinguishing between <jats:styled-content style=\"fixed-case\">IDA<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">TT<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A total of 246 normal subjects, 102 patients with <jats:styled-content style=\"fixed-case\">IDA<\/jats:styled-content> and 115 subjects with <jats:styled-content style=\"fixed-case\">TT<\/jats:styled-content> were accrued for the study. The parameters studied were red blood cell size factor (<jats:styled-content style=\"fixed-case\">RSF<\/jats:styled-content>), low haemoglobin density (<jats:styled-content style=\"fixed-case\">LHD<\/jats:styled-content>%), microcytic anaemia factor (<jats:styled-content style=\"fixed-case\">MAF<\/jats:styled-content>), standard deviation of conductivity of the nonreticulocyte population (<jats:styled-content style=\"fixed-case\">SD<\/jats:styled-content>\u2010C\u2010<jats:styled-content style=\"fixed-case\">NRET<\/jats:styled-content>) and unghosted cell (<jats:styled-content style=\"fixed-case\">UGC<\/jats:styled-content>). Comparison between groups was performed by Student's <jats:italic>t<\/jats:italic>\u2010test, and the diagnostic performance was determined by receiver operating characteristic (<jats:styled-content style=\"fixed-case\">ROC<\/jats:styled-content>) curve analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Both the <jats:styled-content style=\"fixed-case\">LHD<\/jats:styled-content>% and <jats:styled-content style=\"fixed-case\">RSF<\/jats:styled-content> were significantly higher in <jats:styled-content style=\"fixed-case\">IDA<\/jats:styled-content> than <jats:styled-content style=\"fixed-case\">TT<\/jats:styled-content>, whereas <jats:styled-content style=\"fixed-case\">MAF<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">SD<\/jats:styled-content>\u2010C\u2010<jats:styled-content style=\"fixed-case\">NRET<\/jats:styled-content> were significantly lower. The <jats:styled-content style=\"fixed-case\">SD<\/jats:styled-content>\u2010C\u2010<jats:styled-content style=\"fixed-case\">NRET<\/jats:styled-content> showed the best diagnostic performance as a single parameter. A formula, [(<jats:styled-content style=\"fixed-case\">RBC<\/jats:styled-content>\u00a0+\u00a0Hb)\u00a0\u00d7\u00a0(<jats:styled-content style=\"fixed-case\">HCT<\/jats:styled-content>\u00a0+\u00a0<jats:styled-content style=\"fixed-case\">SD<\/jats:styled-content>\u2010C\u2010<jats:styled-content style=\"fixed-case\">NRET<\/jats:styled-content>)]\/<jats:styled-content style=\"fixed-case\">RDW<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">SD<\/jats:styled-content>, was devised to distinguish between <jats:styled-content style=\"fixed-case\">IDA<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">TT<\/jats:styled-content>. With a cut\u2010off value of 23, the area under the curve (<jats:styled-content style=\"fixed-case\">AUC<\/jats:styled-content>) was 0.995 (95% <jats:styled-content style=\"fixed-case\">CI<\/jats:styled-content> of 0.99\u20131.00), the sensitivity was 97%, and the specificity was 99.1%.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The new <jats:styled-content style=\"fixed-case\">RBC<\/jats:styled-content> parameters on Beckman Coulter DxH800 provide useful information in distinguishing between <jats:styled-content style=\"fixed-case\">IDA<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">TT<\/jats:styled-content>, which is important for clinical decision\u2010making and for streamlining laboratory testing. A new formula is devised that performs better than other discriminant functions in the literature.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijlh.12262","type":"journal-article","created":{"date-parts":[[2014,5,28]],"date-time":"2014-05-28T09:39:57Z","timestamp":1401269997000},"page":"199-207","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Evaluation of the new red cell parameters on Beckman Coulter DxH800 in distinguishing iron deficiency anaemia from thalassaemia trait"],"prefix":"10.1111","volume":"37","author":[{"given":"E. H. Y.","family":"Ng","sequence":"first","affiliation":[{"name":"Department of Pathology Hong Kong Sanatorium and Hospital Happy Valley Hong Kong"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. H. 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