{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,9,23]],"date-time":"2023-09-23T23:10:55Z","timestamp":1695510655956},"reference-count":29,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2014,9,1]],"date-time":"2014-09-01T00:00:00Z","timestamp":1409529600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T00:00:00Z","timestamp":1441065600000},"content-version":"tdm","delay-in-days":365,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2015,6]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>The coagulation activation in \u03b2\u2010thalassaemia is multifactorial and most likely a consequence of the exposure of phosphatidylserine (<jats:styled-content style=\"fixed-case\">PS<\/jats:styled-content>) on <jats:styled-content style=\"fixed-case\">RBC<\/jats:styled-content>s surface. The degree of <jats:styled-content style=\"fixed-case\">PS<\/jats:styled-content> exposure and procoagulant activity of <jats:styled-content style=\"fixed-case\">RBC<\/jats:styled-content>s in \u03b2\u2010thalassaemia trait (<jats:styled-content style=\"fixed-case\">BTT<\/jats:styled-content>) subjects carrying common Mediterranean mutations were assessed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Eighty <jats:styled-content style=\"fixed-case\">BTT<\/jats:styled-content> subjects carrying common Mediterranean mutations (\u03b2+, <jats:italic>n\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>53 and \u03b2<jats:sub>0<\/jats:sub>, <jats:italic>n\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>27) and sixty healthy subjects served as controls were studied. Plasma prothrombin fragment 1+2 (F1+2), percentage of <jats:styled-content style=\"fixed-case\">PS<\/jats:styled-content> expression on <jats:styled-content style=\"fixed-case\">RBC<\/jats:styled-content>s membrane, clotting times of modified thromboplastin generation test (<jats:styled-content style=\"fixed-case\">MTGT<\/jats:styled-content>) and modified partial thromboplastin with kaolin (<jats:styled-content style=\"fixed-case\">MPTTK<\/jats:styled-content>) were estimated.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The percentage of annexin V positive <jats:styled-content style=\"fixed-case\">RBC<\/jats:styled-content>s and plasma F1+2 had a significant increase and <jats:styled-content style=\"fixed-case\">MTGT<\/jats:styled-content> had a significant decrease in <jats:styled-content style=\"fixed-case\">BTT<\/jats:styled-content> subjects versus controls and in \u03b2<jats:sub>0<\/jats:sub> group versus \u03b2+ group. <jats:styled-content style=\"fixed-case\">MPTTK<\/jats:styled-content> was significantly shorter in <jats:styled-content style=\"fixed-case\">BTT<\/jats:styled-content> subjects than controls, but no significant deference between <jats:styled-content style=\"fixed-case\">BTT<\/jats:styled-content> subjects. The percentage of annexin V positive <jats:styled-content style=\"fixed-case\">RBC<\/jats:styled-content>s showed a significant negative correlation with haemoglobin level, <jats:styled-content style=\"fixed-case\">MTGT<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">MPTTK<\/jats:styled-content>, and a significant positive correlation with plasma F1+2.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">BTT<\/jats:styled-content> subjects may have a risk of hypercoagulable state particularly in \u03b2<jats:sub>0<\/jats:sub> genotype. Measurement of <jats:styled-content style=\"fixed-case\">PS<\/jats:styled-content> exposure on <jats:styled-content style=\"fixed-case\">RBC<\/jats:styled-content>s and the plasma F1+2 is useful to evaluate hypercoagulability state.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijlh.12289","type":"journal-article","created":{"date-parts":[[2014,9,1]],"date-time":"2014-09-01T15:30:59Z","timestamp":1409585459000},"page":"326-333","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["The hypercoagulable status in common <scp>M<\/scp>editerranean \u03b2\u2010thalassaemia mutations trait"],"prefix":"10.1111","volume":"37","author":[{"given":"H. A.","family":"Labib","sequence":"first","affiliation":[{"name":"Clinical Pathology Department Faculty of Medicine Zagazig University Zagazig Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. L.","family":"Etewa","sequence":"additional","affiliation":[{"name":"Medical Biochemistry Department Faculty of Medicine Zagazig University Zagazig Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Atia","sequence":"additional","affiliation":[{"name":"Internal Medicine Department Faculty of Medicine Zagazig University Zagazig Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,9]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.3109\/03630260903346395"},{"key":"e_1_2_9_3_1","first-page":"277","article-title":"The prevention of thalassemia in Sardinia","volume":"36","author":"Cao A","year":"1989","journal-title":"Clin Genet"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2007.06740.x"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V91.8.3044.3044_3044_3051"},{"key":"e_1_2_9_6_1","first-page":"147","article-title":"The role of phosphatidylserine in recognition and removal of erythrocytes","volume":"50","author":"Kuypers FA","year":"2004","journal-title":"Cell Mol Biol"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2736(80)90579-9"},{"key":"e_1_2_9_8_1","first-page":"248","volume-title":"Practical Haematology","author":"Dacie JV","year":"1984"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.17.3.287"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2141.2001.02482.x"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.13243"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.blre.2008.04.001"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1182\/asheducation-2007.1.68"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V87.3.1179.bloodjournal8731179"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2141.1997.1502965.x"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2141.1999.01758.x"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1177\/1076029607306397"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM200008173430702"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1196\/annals.1345.039"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02983382"},{"key":"e_1_2_9_21_1","first-page":"741","article-title":"Measurement of membrane phospholipid asymmetry in normal and sickle\u2010cell erythrocytes by means of annexin V binding","volume":"123","author":"Tait JF","year":"1994","journal-title":"J Lab Clin Med"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.10915"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1159\/000067907"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI116706"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcmd.2006.06.001"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2000.02376.x"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.11763"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.thromres.2007.02.006"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2141.2002.03711.x"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V96.3.1119.015k53_1119_1124"}],"container-title":["International Journal of Laboratory Hematology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fijlh.12289","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ijlh.12289","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,23]],"date-time":"2023-09-23T22:46:49Z","timestamp":1695509209000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/ijlh.12289"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,9]]},"references-count":29,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2015,6]]}},"alternative-id":["10.1111\/ijlh.12289"],"URL":"https:\/\/doi.org\/10.1111\/ijlh.12289","archive":["Portico"],"relation":{},"ISSN":["1751-5521","1751-553X"],"issn-type":[{"value":"1751-5521","type":"print"},{"value":"1751-553X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,9]]},"assertion":[{"value":"2014-10-13","name":"published_online","label":"Published Online","group":{"name":"publication_history","label":"Publication History"}}]}}