{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,7,10]],"date-time":"2024-07-10T22:35:32Z","timestamp":1720650932575},"reference-count":33,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2014,12,3]],"date-time":"2014-12-03T00:00:00Z","timestamp":1417564800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T00:00:00Z","timestamp":1441065600000},"content-version":"tdm","delay-in-days":272,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2015,8]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>Chronic lymphocytic leukemia is usually diagnosed through the characteristic morphology\/immunophenotype of the lymphocytes, but some <jats:styled-content style=\"fixed-case\">CLL<\/jats:styled-content> cases remain atypical resulting in diagnostic uncertainty.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Using flow cytometry analysis, we investigated the expression of <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>s160\/200 on B cells from 124 patients (82 <jats:styled-content style=\"fixed-case\">CLL<\/jats:styled-content>, 42 other B\u2010cell neoplasms) and nine controls. <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>s160\/200 measurements were determined as a ratio of the mean fluorescence intensities of leukemic cells\/controls and were considered positive when the ratios were \u22652 and 20, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Sixty and 83% <jats:styled-content style=\"fixed-case\">CLL<\/jats:styled-content> expressed <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>s160\/200 as compared to 5% and 10% of other B\u2010cell neoplasms, respectively. None of the controls showed <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>s160\/200 expressions. Combination of both markers was observed in 55% of <jats:styled-content style=\"fixed-case\">CLL<\/jats:styled-content> but only in 2% of other B\u2010cell neoplasms, and absence of both markers occurred in 12% of <jats:styled-content style=\"fixed-case\">CLL<\/jats:styled-content> but in 86% of other B\u2010cell neoplasms.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>s160\/200 were associated with markers of the gold standard \u2018Matutes score\u2019 and could be useful markers to differentiate atypical <jats:styled-content style=\"fixed-case\">CLL<\/jats:styled-content> from other B\u2010cell neoplasms in the absence of available biopsies or cytogenetics and molecular studies.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijlh.12315","type":"journal-article","created":{"date-parts":[[2014,12,4]],"date-time":"2014-12-04T01:12:58Z","timestamp":1417655578000},"page":"486-494","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Combination of <scp>CD<\/scp>160 and <scp>CD<\/scp>200 as a useful tool for differential diagnosis between chronic lymphocytic leukemia and other mature B\u2010cell neoplasms"],"prefix":"10.1111","volume":"37","author":[{"given":"J.\u2010F.","family":"Lesesve","sequence":"first","affiliation":[{"name":"Laboratory of Hematology University Hospital Nancy France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Tardy","sequence":"additional","affiliation":[{"name":"Laboratory of Hematology University Hospital Nancy France"},{"name":"Department of Hematology University Hospital Nancy 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