{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T01:45:10Z","timestamp":1791942310095,"version":"4.3.4"},"reference-count":19,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2015,1,14]],"date-time":"2015-01-14T00:00:00Z","timestamp":1421193600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T00:00:00Z","timestamp":1441065600000},"content-version":"tdm","delay-in-days":230,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2015,8]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>Acute Promyelocytic Leukemia (APL) is a curable malignancy with studies showing above 90% survival. However, population\u2010based studies looking at survival suggest that approximately 30% of patients with <jats:styled-content style=\"fixed-case\">APL<\/jats:styled-content> die during induction. Early demonstration of t(15;17) will lead to accurate decision making regarding treatment. The aim of this project was to validate earlier time frames for the Abbott Molecular Vysis <jats:styled-content style=\"fixed-case\">LSI<\/jats:styled-content> promyelocytic leukemia (<jats:styled-content style=\"fixed-case\">PML<\/jats:styled-content>)\/ retinoic acid receptor alpha (<jats:styled-content style=\"fixed-case\">RARA<\/jats:styled-content>) fluorescence <jats:italic>in situ<\/jats:italic> hybridization (<jats:styled-content style=\"fixed-case\">FISH<\/jats:styled-content>) probe (<jats:styled-content style=\"fixed-case\">ASR<\/jats:styled-content> 6\u201316\u00a0h).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Twenty patients (15 <jats:styled-content style=\"fixed-case\">APL<\/jats:styled-content> cases and five non\u2010<jats:styled-content style=\"fixed-case\">APL<\/jats:styled-content> cases) were selected for validating various hybridization times for the <jats:styled-content style=\"fixed-case\">FISH<\/jats:styled-content> probe. Expected normal signal pattern was two red and two green signals (2R2G), and the most common expected abnormal signal pattern was two fusion (yellow) signals, one red and one green (2F1R1G) and\/or one fusion, one red and one green (1F1R1G).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The specificity of the probe ranged from 84% at 2\u00a0h, 86% at 4\u00a0h, 84% at 6\u00a0h, and 87% for overnight hybridization. The sensitivity increased from 79% at 2\u00a0h, 80% at 4\u00a0h, 81% at 6\u00a0h to 87% for overnight hybridization.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Based on the validation studies, we recommend reading of <jats:styled-content style=\"fixed-case\">FISH<\/jats:styled-content> results at the 4\u2010h incubation mark for a preliminary diagnosis and confirmation with overnight hybridization.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijlh.12326","type":"journal-article","created":{"date-parts":[[2015,1,14]],"date-time":"2015-01-14T20:36:14Z","timestamp":1421267774000},"page":"515-520","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Utility and impact of early t(15;17) identification by Fluorescence <i>In Situ<\/i> Hybridization (<scp>FISH<\/scp>) in clinical decision making for patients in Acute Promyelocytic Leukemia (<scp>APL<\/scp>)"],"prefix":"10.1111","volume":"37","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8283-2403","authenticated-orcid":false,"given":"R.","family":"Kolhe","sequence":"first","affiliation":[{"name":"Department of Pathology Georgia Regents University Augusta GA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Mangaonkar","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine Georgia Regents University Cancer Center Augusta GA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Mansour","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine Georgia Regents University Cancer Center Augusta GA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Clemmons","sequence":"additional","affiliation":[{"name":"University of Georgia College of Pharmacy Augusta GA 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