{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T01:44:14Z","timestamp":1788572654019,"version":"build-2803163510"},"reference-count":28,"publisher":"Wiley","issue":"S1","license":[{"start":{"date-parts":[[2015,5,14]],"date-time":"2015-05-14T00:00:00Z","timestamp":1431561600000},"content-version":"vor","delay-in-days":13,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T00:00:00Z","timestamp":1441065600000},"content-version":"tdm","delay-in-days":123,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2015,5]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Acute leukemia, myelodysplastic syndromes (<jats:styled-content style=\"fixed-case\">MDS<\/jats:styled-content>), myeloproliferative neoplasms and lymphomas are the most prevalent diagnoses in adults presenting with new onset cytopenia. Here, we describe two 10\u2010color panels of surface markers (screening and comprehensive panel) applied at the Flow Cytometry Laboratory, University Health Network, Toronto,<jats:styled-content style=\"fixed-case\">ON<\/jats:styled-content>, Canada. A 10\u2010color flow cytometry is applied using the stain\u2013lyse\u2013wash sample preparation method. In patients with &lt;10% blasts and no clear involvement by hematological malignancy based on cytomorphological evaluation of bone marrow (<jats:styled-content style=\"fixed-case\">BM<\/jats:styled-content>) smear, the recently published one\u2010tube 10\u2010color 14\u2010antibody screening panel is applied. This panel allows detection of major B\u2010 and T\u2010cell abnormalities, enumeration of cells in blast region (<jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>45 dim), and gives insight into myeloid<jats:styled-content style=\"fixed-case\">BM<\/jats:styled-content>compartment, including calculation of four\u2010parameter score for<jats:styled-content style=\"fixed-case\">MDS<\/jats:styled-content>\u2010related abnormalities. In patients who present with \u226510\u00a0\u2013\u00a0&lt;20% blasts in blood or<jats:styled-content style=\"fixed-case\">BM<\/jats:styled-content>smears, a comprehensive three\u2010tube panel of surface markers is used up front. The analysis is focused on the detection of abnormal antigen expression patterns not seen in normal\/reactive<jats:styled-content style=\"fixed-case\">BM<\/jats:styled-content>, according to the guidelines developed by International\/European LeukemiaNet Working Group for Flow Cytometry in<jats:styled-content style=\"fixed-case\">MDS<\/jats:styled-content>. In patients with \u226520% blasts, an additional tube is added to allow the detection of cytoplasmic markers necessary to diagnose mixed phenotype acute leukemia.<\/jats:p>","DOI":"10.1111\/ijlh.12368","type":"journal-article","created":{"date-parts":[[2015,5,15]],"date-time":"2015-05-15T04:33:00Z","timestamp":1431664380000},"page":"133-143","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Flow cytometry immunophenotyping in integrated diagnostics of patients with newly diagnosed cytopenia: one tube 10\u2010color 14\u2010antibody screening panel and 3\u2010tube extensive panel for detection of<scp>MDS<\/scp>\u2010related features"],"prefix":"10.1111","volume":"37","author":[{"given":"A.","family":"Porwit","sequence":"first","affiliation":[{"name":"Department of Pathobiology and Laboratory Medicine University of Toronto University Health Network Toronto ON Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Rajab","sequence":"additional","affiliation":[{"name":"Department of Pathobiology and Laboratory Medicine University of Toronto University Health Network Toronto ON Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,5,14]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1309\/AJCP50AEEYGREWUZ"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.humpath.2012.10.006"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2007-11-120535"},{"key":"e_1_2_9_5_1","volume-title":"WHO Classification of Tumours of Haematopoietic and Lymphoid Tissues","author":"Swerdlow 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