{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,11]],"date-time":"2025-11-11T15:41:16Z","timestamp":1762875676807},"reference-count":24,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2015,5,11]],"date-time":"2015-05-11T00:00:00Z","timestamp":1431302400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T00:00:00Z","timestamp":1441065600000},"content-version":"tdm","delay-in-days":113,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2015,10]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>This study aimed to verify the association between the <jats:styled-content style=\"fixed-case\">JAK<\/jats:styled-content>2 46\/1 haplotype (V617F positive) and some hematological parameters in <jats:styled-content style=\"fixed-case\">BCR<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">ABL<\/jats:styled-content>\u2010negative chronic myeloproliferative neoplasms (<jats:styled-content style=\"fixed-case\">cMPN<\/jats:styled-content>s) in our population.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The blood samples obtained from the patients with <jats:styled-content style=\"fixed-case\">cMPN<\/jats:styled-content> were genotyped for the <jats:styled-content style=\"fixed-case\">JAK<\/jats:styled-content>2 V617F mutation and <jats:styled-content style=\"fixed-case\">JAK<\/jats:styled-content>2 rs10974944 <jats:styled-content style=\"fixed-case\">SNP<\/jats:styled-content> screening using a <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">RFLP<\/jats:styled-content> assay.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The <jats:italic><jats:styled-content style=\"fixed-case\">JAK<\/jats:styled-content>2 V617F<\/jats:italic> mutation was detected in 80.15% of patients. The G variant of rs10974944 was more frequent in all <jats:styled-content style=\"fixed-case\">MPN<\/jats:styled-content>s, especially those that were <jats:italic><jats:styled-content style=\"fixed-case\">JAK<\/jats:styled-content>2<\/jats:italic> V617F positive, than in the control population. We also compared the 46\/1 haplotype status in each <jats:styled-content style=\"fixed-case\">MPN<\/jats:styled-content> disease entity, polycythemia vera (<jats:styled-content style=\"fixed-case\">PV<\/jats:styled-content>), essential thrombocythemia (<jats:styled-content style=\"fixed-case\">ET<\/jats:styled-content>), primary myelofibrosis (<jats:styled-content style=\"fixed-case\">PMF<\/jats:styled-content>), and <jats:styled-content style=\"fixed-case\">MPN<\/jats:styled-content>u with controls. The G allele frequency relative to controls was significantly enriched in patients with <jats:styled-content style=\"fixed-case\">PV<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">ET<\/jats:styled-content>, but not in those with <jats:styled-content style=\"fixed-case\">PMF<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">MPN<\/jats:styled-content>u. <jats:styled-content style=\"fixed-case\">PV<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">ET<\/jats:styled-content> patients especially, all of whom had the <jats:italic><jats:styled-content style=\"fixed-case\">JAK<\/jats:styled-content>2 V617F<\/jats:italic> mutation, showed significant excess of the G allele. The frequency of JAK2 V617F mutation was associated with elevated hematological parameters, but when we analyze the occurrence of the mutation and the presence of the G allele, just the high hemoglobin was significantly.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>In agreement with previous reports, <jats:italic><jats:styled-content style=\"fixed-case\">JAK<\/jats:styled-content>2 46\/1<\/jats:italic> haplotype for <jats:italic><jats:styled-content style=\"fixed-case\">JAK<\/jats:styled-content>2 V617F<\/jats:italic> was associated with <jats:styled-content style=\"fixed-case\">cMPN<\/jats:styled-content> positive in Brazilian patients.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijlh.12380","type":"journal-article","created":{"date-parts":[[2015,5,11]],"date-time":"2015-05-11T09:46:41Z","timestamp":1431337601000},"page":"654-660","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["JAK2 46\/1 haplotype is associated with <scp>JAK<\/scp>2 V617F \u2013 positive myeloproliferative neoplasms in Brazilian patients"],"prefix":"10.1111","volume":"37","author":[{"given":"L. C.","family":"Macedo","sequence":"first","affiliation":[{"name":"Departamento de Ci\u00eancias B\u00e1sicas da Sa\u00fade Universidade Estadual de Maring\u00e1 Maring\u00e1 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B. C.","family":"Santos","sequence":"additional","affiliation":[{"name":"Departamento de Ci\u00eancias B\u00e1sicas da Sa\u00fade Universidade Estadual de Maring\u00e1 Maring\u00e1 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Pagliarini\u2010e\u2010Silva","sequence":"additional","affiliation":[{"name":"Departamento de Ci\u00eancias B\u00e1sicas da Sa\u00fade Universidade Estadual de Maring\u00e1 Maring\u00e1 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K. B. B.","family":"Pagnano","sequence":"additional","affiliation":[{"name":"Centro de Hematologia e Hemoterapia de Campinas Universidade Estadual de Campinas S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"Departamento de Ci\u00eancias B\u00e1sicas da Sa\u00fade Universidade Estadual de Maring\u00e1 Maring\u00e1 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F. C.","family":"Quintero","sequence":"additional","affiliation":[{"name":"Departamento de Ci\u00eancias B\u00e1sicas da Sa\u00fade Universidade Estadual de Maring\u00e1 Maring\u00e1 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. E.","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Hospital do C\u00e2ncer de Maringa Maringa Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E. 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