{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T02:27:25Z","timestamp":1787624845621,"version":"build-2736575974"},"reference-count":32,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2015,5,25]],"date-time":"2015-05-25T00:00:00Z","timestamp":1432512000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T00:00:00Z","timestamp":1441065600000},"content-version":"tdm","delay-in-days":99,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"name":"Blood Transfusion Research Center of High Institute for Research and Education in Transfusion Medicine"}],"content-domain":{"domain":["wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2015,10]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>The <jats:styled-content style=\"fixed-case\">JAK<\/jats:styled-content>2V617F mutation has emerged in recent years as a diagnostic as well as a treatment target in patients with polycythemia vera (<jats:styled-content style=\"fixed-case\">PV<\/jats:styled-content>) and essential thrombocythemia (<jats:styled-content style=\"fixed-case\">ET<\/jats:styled-content>). The disease phenotype is also influenced by other factors such as micro<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> (mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>) deregulation. The aim of this study was to investigate miR\u2010125 expression level in these patients with those obtained from healthy control subjects and its correlation with <jats:styled-content style=\"fixed-case\">JAK<\/jats:styled-content>2 allele burden and laboratory findings.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>In total, forty patients with a clinical diagnosis of <jats:styled-content style=\"fixed-case\">PV<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">ET<\/jats:styled-content> were examined at the time of diagnosis. Ten healthy subjects were checked as controls. We performed <jats:styled-content style=\"fixed-case\">JAK<\/jats:styled-content>2 V617F allele burdens measurement and expression analysis of miR\u2010125b\u20105p, miR\u2010125b\u20103p, miR\u2010125a\u20105p, and miR\u2010125a\u20103p in leukocytes isolated from peripheral blood by quantitative real\u2010time polymerase chain reaction.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>MiR\u2010125b\u20105p and miR\u2010125a\u20105p were upregulated in both patients with <jats:styled-content style=\"fixed-case\">PV<\/jats:styled-content> (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.00 and <jats:italic>P<\/jats:italic>\u00a0=\u00a00.003, respectively) and <jats:styled-content style=\"fixed-case\">ET<\/jats:styled-content> (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.02 and <jats:italic>P<\/jats:italic>\u00a0=\u00a00.002, respectively). In <jats:styled-content style=\"fixed-case\">PV<\/jats:styled-content> group, a significant correlation was observed between miR\u2010125a\u20105p and platelet counts (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.01, <jats:italic>r<\/jats:italic>\u00a0=\u00a00.531). The correlation between mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">JA<\/jats:styled-content>k2 allele burden was not significant.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>In conclusion, our data indicate that other factors such as aberrant miR\u2010125 expression may influence on the disease phenotype in patients with <jats:styled-content style=\"fixed-case\">PV<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">ET<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijlh.12381","type":"journal-article","created":{"date-parts":[[2015,5,25]],"date-time":"2015-05-25T15:02:49Z","timestamp":1432566169000},"page":"661-667","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Expression analysis of micro<scp>RNA<\/scp>\u2010125 in patients with polycythemia vera and essential thrombocythemia and correlation with <scp>JAK<\/scp>2 allele burden and laboratory findings"],"prefix":"10.1111","volume":"37","author":[{"given":"S.","family":"Ferdowsi","sequence":"first","affiliation":[{"name":"Blood Transfusion Research Center High Institute for Research and Education in Transfusion Medicine Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Atarodi","sequence":"additional","affiliation":[{"name":"Blood Transfusion Research Center High Institute for Research and Education in Transfusion Medicine Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N.","family":"Amirizadeh","sequence":"additional","affiliation":[{"name":"Blood Transfusion Research Center High Institute for Research and Education in Transfusion Medicine Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"Toogeh","sequence":"additional","affiliation":[{"name":"Hematology\u2010Oncology and BMT Research Center Imam Khomeini Hospital Tehran University of Medical Sciences Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Azarkeivan","sequence":"additional","affiliation":[{"name":"Blood Transfusion Research Center High Institute for Research and Education in Transfusion Medicine Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Shirkoohi","sequence":"additional","affiliation":[{"name":"Molecular Genetics Cancer Research Center Cancer Institute Imam Khomeini Hospital Complex Tehran University of Medical Sciences Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Faranoush","sequence":"additional","affiliation":[{"name":"Blood Transfusion Research Center High Institute for Research and Education in Transfusion Medicine Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Vaezi","sequence":"additional","affiliation":[{"name":"Hematology\u2010Oncology and Stem Cell Transplantation Research Center Shariati Hospital Tehran University of Medical Sciences Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Alimoghaddam","sequence":"additional","affiliation":[{"name":"Hematology\u2010Oncology and Stem Cell Transplantation Research Center Shariati Hospital Tehran University of Medical Sciences Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Ghavamzadeh","sequence":"additional","affiliation":[{"name":"Hematology\u2010Oncology and Stem Cell Transplantation Research Center Shariati Hospital Tehran University of Medical Sciences Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H. 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