{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,9,23]],"date-time":"2023-09-23T18:11:28Z","timestamp":1695492688943},"reference-count":19,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2015,6,9]],"date-time":"2015-06-09T00:00:00Z","timestamp":1433808000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T00:00:00Z","timestamp":1441065600000},"content-version":"tdm","delay-in-days":84,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2015,10]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>Chronic myeloid leukemia (<jats:styled-content style=\"fixed-case\">CML<\/jats:styled-content>) is typically associated with the Philadelphia chromosome, resulting in the production of <jats:styled-content style=\"fixed-case\">BCR<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">ABL<\/jats:styled-content> fusion oncoprotein with upregulated tyrosine kinase activity. We aimed to evaluate a new flow cytometric immunobead assay to detect <jats:styled-content style=\"fixed-case\">BCR<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">ABL<\/jats:styled-content> protein in a group of patients with <jats:styled-content style=\"fixed-case\">CML<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We enrolled 49 patients with <jats:styled-content style=\"fixed-case\">CML<\/jats:styled-content>, whose <jats:styled-content style=\"fixed-case\">qRT<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> and\/or cytogenetic analysis of Philadelphia chromosome aberration was available, including tyrosine kinase inhibitors (<jats:styled-content style=\"fixed-case\">TKI<\/jats:styled-content>s)\u2010na\u00efve and (<jats:styled-content style=\"fixed-case\">TKI<\/jats:styled-content>s)\u2010treated patients with various levels of response. Twenty Philadelphia\u2010negative healthy individuals were also enrolled to obtain analytical negative controls. Peripheral blood samples were analyzed for <jats:styled-content style=\"fixed-case\">BCR<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">ABL<\/jats:styled-content> fusion protein by flow cytometry.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The <jats:styled-content style=\"fixed-case\">BCR<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">ABL<\/jats:styled-content> fusion protein flow cytometric assay seemed efficacious to both diagnose the presence (<jats:italic>P<\/jats:italic>\u2010value &lt;0.0001) and distinguish the levels (<jats:italic>P<\/jats:italic>\u2010value\u00a0=\u00a00) of the Philadelphia chromosome aberration. Groups of <jats:styled-content style=\"fixed-case\">TKI<\/jats:styled-content>\u2010na\u00efve and <jats:styled-content style=\"fixed-case\">TKI<\/jats:styled-content>\u2010treated patients as well as levels of molecular\/cytogenetic response to <jats:styled-content style=\"fixed-case\">TKI<\/jats:styled-content>\u2010therapy were effectively discriminated (<jats:italic>P<\/jats:italic>\u2010value &lt;0.01). The receiver operating characteristic (<jats:styled-content style=\"fixed-case\">ROC<\/jats:styled-content>) curve analysis indicated the diagnostic value of the assay as excellent (<jats:styled-content style=\"fixed-case\">AUC<\/jats:styled-content>\u00a0=\u00a00.95, <jats:italic>P<\/jats:italic>\u2010value\u00a0=\u00a00).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The evaluated <jats:styled-content style=\"fixed-case\">BCR<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">ABL<\/jats:styled-content> fusion protein assay might be useful for diagnosing and monitoring Philadelphia chromosome aberration.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijlh.12394","type":"journal-article","created":{"date-parts":[[2015,6,11]],"date-time":"2015-06-11T03:45:18Z","timestamp":1433994318000},"page":"723-728","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Reconsideration of <scp>BCR<\/scp>\u2010<scp>ABL<\/scp> protein flow cytometric immunobead assay: how potent to diagnose and monitor chronic myeloid leukemia?"],"prefix":"10.1111","volume":"37","author":[{"given":"R.","family":"Kelani","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Microbiology Faculty of Pharmacy Damascus University Damascus Syria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F.","family":"Monem","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Microbiology Faculty of Pharmacy Damascus University Damascus Syria"},{"name":"Clinical Laboratories Department AL\u2010Assad Hospital Damascus University Damascus Syria"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,6,9]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.2146\/ajhp070484"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2141.2002.03705.x"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/1741-7015-9-99"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.beha.2010.09.010"},{"key":"e_1_2_7_6_1","first-page":"195","article-title":"An accurate and rapid flow cytometric diagnosis of BCR ABL fusion protein an alternative way to detect the different phases of chronic myeloid leukemia","volume":"3","author":"Dasgupta S","year":"2013","journal-title":"Int J Adv Sci Tech 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