{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T21:45:23Z","timestamp":1792791923187,"version":"4.5.0"},"reference-count":38,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2015,7,20]],"date-time":"2015-07-20T00:00:00Z","timestamp":1437350400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T00:00:00Z","timestamp":1441065600000},"content-version":"tdm","delay-in-days":43,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2015,12]]},"abstract":"Summary<\/jats:title>\n \n Introduction<\/jats:title>\n \n Philadelphia\u2010negative chronic myeloproliferative neoplasms (\n MPN<\/jats:styled-content>\n s) are clonal disorders that present\n \n JAK<\/jats:styled-content>\n 2\n <\/jats:italic>\n \n V617F<\/jats:italic>\n <\/jats:sup>\n mutation in 50\u201395% of cases. The main objective of this study was the comparison of two\n PCR<\/jats:styled-content>\n methods, real\u2010time (\n qPCR<\/jats:styled-content>\n ) and droplet digital\n PCR<\/jats:styled-content>\n (\n DD<\/jats:styled-content>\n \u2010\n PCR<\/jats:styled-content>\n ) for detection of the\n \n JAK<\/jats:styled-content>\n 2\n <\/jats:italic>\n \n V617F<\/jats:italic>\n <\/jats:sup>\n mutation, to assess analytic sensitivity, specificity, and feasibility of the two methods.\n <\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n Ninety\u2010nine patients with\n MPN<\/jats:styled-content>\n of 225 presenting the\n \n JAK<\/jats:styled-content>\n 2\n <\/jats:italic>\n \n V617F<\/jats:italic>\n <\/jats:sup>\n mutation by\n qPCR<\/jats:styled-content>\n have been evaluated by\n DD<\/jats:styled-content>\n \u2010\n PCR<\/jats:styled-content>\n also.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n We demonstrated an absolute concordance in terms of specificity between the two methods,\n DD<\/jats:styled-content>\n \u2010\n PCR<\/jats:styled-content>\n showing a higher sensitivity (half a log higher than\n qPCR<\/jats:styled-content>\n ). As expected, a progressive increase of mutant allele burden was observed from essential thrombocythemia (\n ET<\/jats:styled-content>\n ) to polycythemia vera (\n PV<\/jats:styled-content>\n ) and primary myelofibrosis (\n PMF<\/jats:styled-content>\n ) to secondary myelofibrosis (\n SMF<\/jats:styled-content>\n ).\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n \n In conclusion, our study showed that\n DD<\/jats:styled-content>\n \u2010\n PCR<\/jats:styled-content>\n could represent a new and promising technological evolution for detection of\n \n JAK<\/jats:styled-content>\n 2\n <\/jats:italic>\n mutation in\n MPN<\/jats:styled-content>\n s.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/ijlh.12404","type":"journal-article","created":{"date-parts":[[2015,7,20]],"date-time":"2015-07-20T01:27:50Z","timestamp":1437355670000},"page":"766-773","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["Real\u2010Time\n PCR<\/scp>\n and Droplet Digital\n PCR<\/scp>\n : two techniques for detection of the\n \n JAK<\/scp>\n 2\n <\/i>\n \n V617F<\/i>\n <\/sup>\n mutation in Philadelphia\u2010negative chronic myeloproliferative neoplasms"],"prefix":"10.1111","volume":"37","author":[{"given":"G.","family":"Fontanelli","sequence":"first","affiliation":[{"name":"U.O. Hematology Department of Clinical and Experimental Medicine University of Pisa Pisa Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Barat\u00e8","sequence":"additional","affiliation":[{"name":"U.O. Hematology Department of Clinical and Experimental Medicine University of Pisa Pisa Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Ciabatti","sequence":"additional","affiliation":[{"name":"U.O. Hematology Department of Clinical and Experimental Medicine University of Pisa Pisa Italy"},{"name":"GenOMEC University of Siena Siena Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"Guerrini","sequence":"additional","affiliation":[{"name":"U.O. Hematology Department of Clinical and Experimental Medicine University of Pisa Pisa Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Grassi","sequence":"additional","affiliation":[{"name":"U.O. Hematology Department of Clinical and Experimental Medicine University of Pisa Pisa Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Del Re","sequence":"additional","affiliation":[{"name":"U.O. Pharmacology Department of Clinical and Experimental Medicine University of Pisa Pisa Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Morganti","sequence":"additional","affiliation":[{"name":"Department of Clinical and Experimental Medicine University of Pisa Pisa Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I.","family":"Petrini","sequence":"additional","affiliation":[{"name":"Department of Translational Research and New Technologies in Medicine University of Pisa Pisa Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Arici","sequence":"additional","affiliation":[{"name":"U.O. Hematology Department of Clinical and Experimental Medicine University of Pisa Pisa Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Barsotti","sequence":"additional","affiliation":[{"name":"U.O. Hematology Department of Clinical and Experimental Medicine University of Pisa Pisa Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. R.","family":"Metelli","sequence":"additional","affiliation":[{"name":"U.O. Hematology Department of Clinical and Experimental Medicine University of Pisa Pisa Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Danesi","sequence":"additional","affiliation":[{"name":"U.O. Pharmacology Department of Clinical and Experimental Medicine University of Pisa Pisa Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Galimberti","sequence":"additional","affiliation":[{"name":"U.O. 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