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This study aimed to develop a cost\u2010effective, multiplex, genotyping method for detecting common mutations in the <jats:italic>G6<jats:styled-content style=\"fixed-case\">PD<\/jats:styled-content><\/jats:italic> gene.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We used a <jats:styled-content style=\"fixed-case\">SN<\/jats:styled-content>aPshot approach to genotype multiple G6<jats:styled-content style=\"fixed-case\">PD<\/jats:styled-content> mutations that are common to human populations in South\u2010East Asia. This assay is based on multiplex <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> coupled with primer extension reactions. Different G6<jats:styled-content style=\"fixed-case\">PD<\/jats:styled-content> gene mutations were determined by peak retention time and colors of the primer extension products.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We designed <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> primers for multiplex amplification of the <jats:italic>G6<jats:styled-content style=\"fixed-case\">PD<\/jats:styled-content><\/jats:italic> gene fragments and for primer extension reactions to genotype 11 <jats:italic>G6<jats:styled-content style=\"fixed-case\">PD<\/jats:styled-content><\/jats:italic> mutations. <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> samples from a total of 120 unrelated G6<jats:styled-content style=\"fixed-case\">PD<\/jats:styled-content>\u2010deficient individuals from the China\u2013Myanmar border area were used to establish and validate this method. Direct sequencing of the <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> products demonstrated 100% concordance between the\u00a0<jats:styled-content style=\"fixed-case\">SN<\/jats:styled-content>aPshot and the sequencing results.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The <jats:styled-content style=\"fixed-case\">SN<\/jats:styled-content>aPshot method offers a specific and sensitive alternative for simultaneously interrogating multiple G6<jats:styled-content style=\"fixed-case\">PD<\/jats:styled-content> mutations.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijlh.12405","type":"journal-article","created":{"date-parts":[[2015,7,20]],"date-time":"2015-07-20T05:27:30Z","timestamp":1437370050000},"page":"739-745","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["A multiplex method for detection of glucose\u20106\u2010phosphate dehydrogenase (G6<scp>PD<\/scp>) gene 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