{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T20:37:29Z","timestamp":1790973449675,"version":"4.1.0"},"reference-count":18,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2016,6,16]],"date-time":"2016-06-16T00:00:00Z","timestamp":1466035200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T00:00:00Z","timestamp":1441065600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"name":"Diagnostica Stago"}],"content-domain":{"domain":["wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2016,8]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>Haemolysis, icterus and lipaemia (<jats:styled-content style=\"fixed-case\">HIL<\/jats:styled-content>) may affect haemostasis test results. This may be influenced by the level of interfering substance and the reagents and end\u2010point detection system used.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We assessed the influence of <jats:styled-content style=\"fixed-case\">HIL<\/jats:styled-content> on prothrombin time, activated partial thromboplastin time and fibrinogen assay using a viscosity\u2010based detection analyser.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Spontaneous haemolysis that occurred during sample collection and processing had no effect on <jats:styled-content style=\"fixed-case\">PT<\/jats:styled-content> with either a rabbit tissue factor extract or recombinant human tissue factor reagents. In contrast, addition of mechanically haemolysed cells impacted on <jats:styled-content style=\"fixed-case\">PT<\/jats:styled-content> for the highest haemoglobin concentration.<\/jats:p><jats:p>For <jats:styled-content style=\"fixed-case\">APTT<\/jats:styled-content>s determined with <jats:styled-content style=\"fixed-case\">STA<\/jats:styled-content>\u00ae\u2010Cephascreen\u00ae reagent, there was no significant difference between results in haemolysed and nonhaemolysed samples. For the other two reagents studied, <jats:styled-content style=\"fixed-case\">APTT<\/jats:styled-content>s were statistically significantly shorter in haemolysed samples compared with nonhaemolysed samples. This bias was clinically significant only for <jats:styled-content style=\"fixed-case\">STA<\/jats:styled-content>\u00ae\u2010<jats:styled-content style=\"fixed-case\">PTT<\/jats:styled-content> Automate. For all three <jats:styled-content style=\"fixed-case\">APTT<\/jats:styled-content> reagents, the impact of haemolysis was sufficient to impact patient management decisions, and in some samples, the effects of lipaemia and icterus were not clinically significant.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Overall, our results confirm that <jats:styled-content style=\"fixed-case\">PT<\/jats:styled-content> and fibrinogen were not clinically significantly affected by <jats:styled-content style=\"fixed-case\">HIL<\/jats:styled-content>. The <jats:styled-content style=\"fixed-case\">APTT<\/jats:styled-content>s of some haemolysed samples were falsely normal. Haemolysed samples for <jats:styled-content style=\"fixed-case\">APTT<\/jats:styled-content> determination should be rejected.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijlh.12498","type":"journal-article","created":{"date-parts":[[2016,6,16]],"date-time":"2016-06-16T06:59:24Z","timestamp":1466060364000},"page":"375-388","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":43,"title":["Effects of haemolysis, icterus and lipaemia on coagulation tests as performed on Stago STA\u2010Compact\u2010Max analyser"],"prefix":"10.1111","volume":"38","author":[{"given":"A.","family":"Woolley","sequence":"first","affiliation":[{"name":"Sheffield Haemophilia and Thrombosis Centre Sheffield Teaching Hospitals NHS Trust Sheffield UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.\u2010L.","family":"Golmard","sequence":"additional","affiliation":[{"name":"Department de Biostatistiques ER4\/EA3974 Universit\u00e9 Paris 6 et APHP UF 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