{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T14:14:34Z","timestamp":1740147274426,"version":"3.37.3"},"reference-count":20,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2016,6,16]],"date-time":"2016-06-16T00:00:00Z","timestamp":1466035200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T00:00:00Z","timestamp":1441065600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100004320","name":"Shiraz University of Medical Sciences","doi-asserted-by":"publisher","award":["91\u201001\u201045\u20104385"],"award-info":[{"award-number":["91\u201001\u201045\u20104385"]}],"id":[{"id":"10.13039\/501100004320","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004320","name":"Shiraz University of Medical Sciences","doi-asserted-by":"publisher","award":["91-01-45-4385"],"award-info":[{"award-number":["91-01-45-4385"]}],"id":[{"id":"10.13039\/501100004320","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2016,8]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>We assessed the feasibility of flow cytometry\u2013fluorescent <jats:italic>in situ<\/jats:italic> hybridization technique in the detection of translocated <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> in the cytoplasm of human peripheral blood nucleated cells. It is assumed that this assay can be applied as a diagnostic method in the detection of chromosomal translocation which commonly occurs in hematologic malignancies.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">KCL<\/jats:styled-content>\u201022 cell line and white blood cells from 21 <jats:styled-content style=\"fixed-case\">CML<\/jats:styled-content> patients were recruited in the study. Cells were isolated and fixed. After permeabilization, cells were resuspended in hybridization buffer and probes were added to the mixture. Subsequently, cells were washed and analyzed on the flow cytometer instrument. The flow cytometry results were compared with <jats:styled-content style=\"fixed-case\">qRT<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> and fluorescent microscope outcomes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Using the current principle, 97 \u00b1 2.1% of the <jats:styled-content style=\"fixed-case\">KCL<\/jats:styled-content>\u201022 cells were labeled with b2a2 <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content>\u2010specific probes. In addition, seven patients were recognized positive for t(9;22) b2a2. The percentage of cells containing abovementioned translocation in these patients was varied from 3.26% to 97.8%. There was no false\u2010positive result in negative controls (K562 with <jats:styled-content style=\"fixed-case\">BCR<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">ABL<\/jats:styled-content>1 b3a2, <jats:styled-content style=\"fixed-case\">NB<\/jats:styled-content>4, and Jurkat cell lines) along with blood samples of normal controls. All the results obtained by flow\u2010<jats:styled-content style=\"fixed-case\">FISH<\/jats:styled-content> were confirmed by <jats:styled-content style=\"fixed-case\">qRT<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> and fluorescent microscope.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This strategy benefits from appropriate specificity, sensitivity, rapidity, and ability in the determination of malignant cell percentage. Therefore, it can improve traditional time\u2010consuming and labor\u2010intensive <jats:styled-content style=\"fixed-case\">FISH<\/jats:styled-content> method.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijlh.12515","type":"journal-article","created":{"date-parts":[[2016,6,16]],"date-time":"2016-06-16T06:59:21Z","timestamp":1466060361000},"page":"403-411","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Detection of t(9;22) b2a2 fusion transcript by flow cytometry"],"prefix":"10.1111","volume":"38","author":[{"given":"R.","family":"Ranjbaran","sequence":"first","affiliation":[{"name":"Diagnostic Laboratory Sciences and Technology Research Center School of Paramedical Sciences Shiraz University of Medical Sciences Shiraz Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. A.","family":"Okhovat","sequence":"additional","affiliation":[{"name":"Diagnostic Laboratory Sciences and Technology Research Center School of Paramedical Sciences Shiraz University of Medical Sciences Shiraz Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Abbasi","sequence":"additional","affiliation":[{"name":"Diagnostic Laboratory Sciences and Technology Research Center School of Paramedical Sciences Shiraz University of Medical Sciences Shiraz Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"Moezzi","sequence":"additional","affiliation":[{"name":"Diagnostic Laboratory Sciences and Technology Research Center School of Paramedical Sciences Shiraz University of Medical Sciences Shiraz Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F.","family":"Aboualizadeh","sequence":"additional","affiliation":[{"name":"Diagnostic Laboratory Sciences and Technology Research Center School of Paramedical Sciences Shiraz University of Medical Sciences Shiraz Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Z.","family":"Amidzadeh","sequence":"additional","affiliation":[{"name":"Diagnostic Laboratory Sciences and Technology Research Center School of Paramedical Sciences Shiraz University of Medical Sciences Shiraz Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H. 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