{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T10:46:01Z","timestamp":1761648361332},"reference-count":31,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2016,6,20]],"date-time":"2016-06-20T00:00:00Z","timestamp":1466380800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T00:00:00Z","timestamp":1441065600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"name":"Centre of Postgraduate Medical Education","award":["506-1-26-02-13"],"award-info":[{"award-number":["506-1-26-02-13"]}]}],"content-domain":{"domain":["wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2016,8]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>In various countries, standard doses of anti\u2010D IgG used for postpartum immunoprophylaxis of hemolytic disease of fetus and newborn (<jats:styled-content style=\"fixed-case\">HDFN<\/jats:styled-content>) vary from 100 \u03bcg to 300 \u03bcg. There are also different regulations concerning <jats:styled-content style=\"fixed-case\">FMH<\/jats:styled-content> assessment, and opinions about applicable tests are inconclusive.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Three flow cytometry tests (<jats:styled-content style=\"fixed-case\">FCT<\/jats:styled-content>s) with anti\u2010D, anti\u2010HbF, anti\u2010HbF+<jats:styled-content style=\"fixed-case\">CA<\/jats:styled-content> antibodies, and two modifications of microscopic Kleihauer\u2013Betke test (<jats:styled-content style=\"fixed-case\">KBT<\/jats:styled-content>) were used.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In all artificial mixtures with known concentrations, <jats:styled-content style=\"fixed-case\">FCT<\/jats:styled-content>s and <jats:styled-content style=\"fixed-case\">KBT<\/jats:styled-content> with counting 10 000 <jats:styled-content style=\"fixed-case\">RBC<\/jats:styled-content>s had similar satisfying sensitivity and specificity. <jats:styled-content style=\"fixed-case\">KBT<\/jats:styled-content> with counting 2000 <jats:styled-content style=\"fixed-case\">RBC<\/jats:styled-content>s had to be disqualified because of significant discrepancies between expected and measured values of <jats:styled-content style=\"fixed-case\">FMH<\/jats:styled-content>. The test procedure with anti\u2010D was easier and shorter than the remaining tests, but it can be only used for <jats:styled-content style=\"fixed-case\">FMH<\/jats:styled-content> assessment in RhD\u2010negative mothers with RhD\u2010positive newborns. In one clinical sample, it was impossible to distinguish fetal <jats:styled-content style=\"fixed-case\">RBC<\/jats:styled-content>s from maternal F cells in <jats:styled-content style=\"fixed-case\">KBT<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">FC<\/jats:styled-content> with anti\u2010HbF but other tests were useful.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>In the four tests, correlation between expected and obtained results was appropriate (<jats:styled-content style=\"fixed-case\">CCC<\/jats:styled-content> \u03341). Each test had some advantage and limitation in any clinical situation. Therefore, it is best to have opportunity to perform two or three assays in the laboratory.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijlh.12518","type":"journal-article","created":{"date-parts":[[2016,6,20]],"date-time":"2016-06-20T05:47:12Z","timestamp":1466401632000},"page":"419-425","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Quantitative fetomaternal hemorrhage assessment with the use of five laboratory tests"],"prefix":"10.1111","volume":"38","author":[{"given":"A.","family":"Gielezynska","sequence":"first","affiliation":[{"name":"Department of Immunohaematology Centre of Postgraduate Medical Education Warsaw Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Stachurska","sequence":"additional","affiliation":[{"name":"Department of Immunohaematology Centre of Postgraduate Medical Education Warsaw Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Fabijanska\u2010Mitek","sequence":"additional","affiliation":[{"name":"Department of Immunohaematology Centre of Postgraduate Medical Education Warsaw Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Debska","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynaecology Centre of Postgraduate Medical Education Warsaw Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Muzyka","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynaecology Centre of Postgraduate Medical Education Warsaw Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.","family":"Kraszewska","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology Centre of Postgraduate Medical Education Warsaw Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,6,20]]},"reference":[{"key":"e_1_2_8_2_1","first-page":"283","volume-title":"Immunohematology: Principles and Practice","author":"Butch SH","year":"2011"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.22255"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/9781118689943"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.beha.2005.03.003"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/tme.12091"},{"key":"e_1_2_8_7_1","first-page":"1","volume-title":"The Use of anti\u2010D Immunoglobulin for Rhesus D Prophylaxis","author":"Royal College of Obstetricians and Gynaecologists","year":"2011"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2011.03362.x"},{"key":"e_1_2_8_9_1","first-page":"1","volume-title":"The Use of Anti\u2010D Immunoglobulin for the Prevention of RhD Haemolytic Disease of Newborn","author":"Royal College of Physicians of Ireland","year":"2012"},{"key":"e_1_2_8_10_1","first-page":"1","article-title":"Prevention of hemolytic disease of the newborn due to anti\u2010D. 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