{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,11]],"date-time":"2026-07-11T10:15:56Z","timestamp":1783764956763,"version":"3.55.0"},"reference-count":18,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2016,6,24]],"date-time":"2016-06-24T00:00:00Z","timestamp":1466726400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T00:00:00Z","timestamp":1441065600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001407","name":"Department of Biotechnology , Ministry of Science and Technology","doi-asserted-by":"publisher","award":["DBT\/PR14802\/MED\/12\/494\/2010"],"award-info":[{"award-number":["DBT\/PR14802\/MED\/12\/494\/2010"]}],"id":[{"id":"10.13039\/501100001407","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001407","name":"Department of Biotechnology , Ministry of Science and Technology","doi-asserted-by":"publisher","award":["DBT\/PR14802\/MED\/12\/494\/2010"],"award-info":[{"award-number":["DBT\/PR14802\/MED\/12\/494\/2010"]}],"id":[{"id":"10.13039\/501100001407","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2016,10]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>Laboratory diagnosis of hereditary spherocytosis (<jats:styled-content style=\"fixed-case\">HS<\/jats:styled-content>) relies on increased incubated red cell osmotic fragility test for screening. We evaluated the diagnostic role of eosin\u20105\u2032\u2010maleimide (<jats:styled-content style=\"fixed-case\">EMA<\/jats:styled-content>) binding test by flow cytometry in spherocytic and microcytic hypochromic hematological disorders in North Indians.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">EMA<\/jats:styled-content> binding test using flow cytometry was performed on 55 <jats:styled-content style=\"fixed-case\">HS<\/jats:styled-content> (40 families), 26 iron deficiency anemia (<jats:styled-content style=\"fixed-case\">IDA<\/jats:styled-content>), 32 \u03b2\u2010thalassemia trait (\u03b2<jats:styled-content style=\"fixed-case\">TT<\/jats:styled-content>), and 10 autoimmune hemolytic anemia (<jats:styled-content style=\"fixed-case\">AIHA<\/jats:styled-content>) cases and 121 normals. Mean channel fluorescence (<jats:styled-content style=\"fixed-case\">MCF<\/jats:styled-content>) and coefficient of variation (<jats:styled-content style=\"fixed-case\">CV<\/jats:styled-content>) were studied. Different <jats:styled-content style=\"fixed-case\">MCF<\/jats:styled-content> parameters (<jats:styled-content style=\"fixed-case\">MCF<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> MCF<\/jats:styled-content> ratio, percent decrease <jats:styled-content style=\"fixed-case\">MCF<\/jats:styled-content>) and percent increase in <jats:styled-content style=\"fixed-case\">CV<\/jats:styled-content> were analyzed. Receiver operating characteristics analysis was performed to determine best cutoff values, sensitivity, and specificity for discriminating <jats:styled-content style=\"fixed-case\">HS<\/jats:styled-content> from other red cell disorders.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">MCF<\/jats:styled-content> ratio of <jats:styled-content style=\"fixed-case\">HS<\/jats:styled-content> group was significantly lower than normals (0.67 \u00b1 0.07 <jats:italic>vs<\/jats:italic>. 1.01 \u00b1 0.05, <jats:italic>P<\/jats:italic> &lt; 0.001) and other cases. All patients with <jats:styled-content style=\"fixed-case\">HS<\/jats:styled-content> showed <jats:styled-content style=\"fixed-case\">MCF<\/jats:styled-content> ratio to be \u22640.79. Four postsplenectomy cases with near\u2010normal hemograms also revealed low <jats:styled-content style=\"fixed-case\">MCF<\/jats:styled-content> ratio, showing the specificity of the test.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">EMA<\/jats:styled-content> assay was efficient to diagnose cases of <jats:styled-content style=\"fixed-case\">HS<\/jats:styled-content> including postsplenectomy cases and shows no overlap with <jats:styled-content style=\"fixed-case\">IDA<\/jats:styled-content>, \u03b2<jats:styled-content style=\"fixed-case\">TT<\/jats:styled-content>, and <jats:styled-content style=\"fixed-case\">AIHA<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijlh.12533","type":"journal-article","created":{"date-parts":[[2016,6,24]],"date-time":"2016-06-24T11:27:33Z","timestamp":1466767653000},"page":"520-526","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["A comparative evaluation of Eosin\u20105\u2032\u2010maleimide flow cytometry reveals a high diagnostic efficacy for hereditary spherocytosis"],"prefix":"10.1111","volume":"38","author":[{"given":"P.","family":"Joshi","sequence":"first","affiliation":[{"name":"Department of Hematology Postgraduate Institute of Medical Education and Research Chandigarh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Aggarwal","sequence":"additional","affiliation":[{"name":"Department of Hematology Postgraduate Institute of Medical Education and Research Chandigarh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Jamwal","sequence":"additional","affiliation":[{"name":"Department of Hematology Postgraduate Institute of Medical Education and Research Chandigarh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. 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