{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T16:15:47Z","timestamp":1770912947331,"version":"3.50.1"},"reference-count":13,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2016,7,27]],"date-time":"2016-07-27T00:00:00Z","timestamp":1469577600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T00:00:00Z","timestamp":1441065600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100008792","name":"Novartis Pharma","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008792","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008792","name":"Novartis Pharma","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008792","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2016,12]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>Standardization of <jats:italic><jats:styled-content style=\"fixed-case\">BCR<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">ABL<\/jats:styled-content>1<\/jats:italic> messenger RNA quantification by real\u2010time <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> on the International Scale (<jats:styled-content style=\"fixed-case\">IS<\/jats:styled-content>) is critical for monitoring therapy response in chronic myelogenous leukaemia. Since 2006, <jats:italic><jats:styled-content style=\"fixed-case\">BCR<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">ABL<\/jats:styled-content>1 <\/jats:italic><jats:styled-content style=\"fixed-case\">IS<\/jats:styled-content> standardization is propagated along reference laboratories by calculating a laboratory\u2010specific conversion factor (<jats:styled-content style=\"fixed-case\">CF<\/jats:styled-content>), co\u2010ordinated in Europe through the European Treatment and Outcome Study project. Although this process has proven successful to some extent, it has not been achievable for all laboratories due to the complexity of the process and the stringent requirements in terms of numbers of samples to be exchanged. In addition, several <jats:italic><jats:styled-content style=\"fixed-case\">BCR<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">ABL<\/jats:styled-content>1 <\/jats:italic><jats:styled-content style=\"fixed-case\">IS<\/jats:styled-content> quantification methods and secondary reference materials became commercially available. However, it was observed that different <jats:styled-content style=\"fixed-case\">IS<\/jats:styled-content> methods generate consistently different results.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>To overcome these difficulties, we have developed an alternative and simple approach of <jats:styled-content style=\"fixed-case\">CF<\/jats:styled-content> calculation, based on the retrospective analysis of existing external quality assessment (<jats:styled-content style=\"fixed-case\">EQA<\/jats:styled-content>) data. Our approach does not depend on the exchange of samples and is solely based on the mathematical <jats:styled-content style=\"fixed-case\">CF<\/jats:styled-content> calculation using <jats:styled-content style=\"fixed-case\">EQA<\/jats:styled-content> results.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results and conclusion<\/jats:title><jats:p>We have demonstrated by thorough statistical validation that this approach performs well in converting <jats:italic><jats:styled-content style=\"fixed-case\">BCR<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">ABL<\/jats:styled-content>1<\/jats:italic> measurements to improve <jats:styled-content style=\"fixed-case\">IS<\/jats:styled-content> estimation. In expectation of a true golden standard method for <jats:italic><jats:styled-content style=\"fixed-case\">BCR<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">ABL<\/jats:styled-content>1 <\/jats:italic><jats:styled-content style=\"fixed-case\">IS<\/jats:styled-content> quantification, the proposed method is a valuable alternative.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijlh.12556","type":"journal-article","created":{"date-parts":[[2016,7,27]],"date-time":"2016-07-27T11:46:54Z","timestamp":1469620014000},"page":"674-684","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["A novel approach for <i><scp>BCR<\/scp>\u2010<scp>ABL<\/scp>1<\/i> standardization to improve International Scale estimation"],"prefix":"10.1111","volume":"38","author":[{"given":"B.","family":"Maes","sequence":"first","affiliation":[{"name":"Laboratory for Molecular Diagnostics Jessa Hospital Hasselt 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