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Colony\u2010forming cells (CFC<\/jats:styled-content>) and endogenous colonies (EC<\/jats:styled-content>), from bone marrow (BM<\/jats:styled-content>) and\/or peripheral blood (PB<\/jats:styled-content>), were analyzed by colony assay based on methylcellulose. The level of circulating CD<\/jats:styled-content>34+<\/jats:sup> cells was determined by flow cytometry.<\/jats:p><\/jats:sec>Results<\/jats:title>The total number of PB CFC<\/jats:styled-content> in primary myelofibrosis (PMF<\/jats:styled-content>) was increased compared to the control sample (P<\/jats:italic> < 0.01) and essential thrombocythemia (ET<\/jats:styled-content>) (P<\/jats:italic> < 0.05). The highest number of BM<\/jats:styled-content> and PB EC<\/jats:styled-content> was observed in polycythemia vera (PV<\/jats:styled-content>) (P<\/jats:italic> < 0.01). Increased levels of CD<\/jats:styled-content>34+<\/jats:sup> cells characterized early\u2010prefibrotic (57%) and advanced\u2010fibrotic PMF<\/jats:styled-content> (90%) as compared to PV<\/jats:styled-content> (34%) and ET<\/jats:styled-content> (32%) (P<\/jats:italic> < 0.01). In the whole Ph\u2212<\/jats:sup>MPN<\/jats:styled-content> group, the total number of PB CFC<\/jats:styled-content> (P<\/jats:italic> < 0.01), PB EC<\/jats:styled-content> (P<\/jats:italic> < 0.05), and CD<\/jats:styled-content>34+<\/jats:sup> cells (P<\/jats:italic> < 0.01) correlated with the degree of BM<\/jats:styled-content> fibrosis. Higher levels of circulating CD<\/jats:styled-content>34+<\/jats:sup> cells in PMF<\/jats:styled-content> correlated with the total number of PB EC<\/jats:styled-content> (P<\/jats:italic> < 0.05) and degree of BM<\/jats:styled-content> fibrosis (P<\/jats:italic> < 0.01).<\/jats:p><\/jats:sec>Conclusions<\/jats:title>Exploration of the PB<\/jats:styled-content> proliferative characteristics of Ph\u2212<\/jats:sup>MPN<\/jats:styled-content> on diagnosis may be helpful in revealing early\u2010prefibrotic PMF<\/jats:styled-content>. Monitoring the levels of circulating CD<\/jats:styled-content>34+<\/jats:sup> cells may provide a sensitive indicator of fibrotic evolution in PV<\/jats:styled-content> and PMF<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijlh.12564","type":"journal-article","created":{"date-parts":[[2016,12,27]],"date-time":"2016-12-27T04:12:58Z","timestamp":1482811978000},"page":"21-31","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Proliferative characteristics of Philadelphia\u2010negative myeloproliferative neoplasms \u2010 clinical implications"],"prefix":"10.1111","volume":"39","author":[{"given":"D.","family":"\u0160efer","sequence":"first","affiliation":[{"name":"Outpatient Clinic and Diagnostic Department Clinic of Hematology Clinical Center of Serbia Belgrade 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