{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T02:19:11Z","timestamp":1790129951897,"version":"4.0.1"},"reference-count":23,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2016,12,15]],"date-time":"2016-12-15T00:00:00Z","timestamp":1481760000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2016,12,15]],"date-time":"2016-12-15T00:00:00Z","timestamp":1481760000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1"},{"start":{"date-parts":[[2016,12,15]],"date-time":"2016-12-15T00:00:00Z","timestamp":1481760000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions"}],"funder":[{"name":"National Research University Project, Office of Higher Education Commission","award":["WCU-58-001-HR"],"award-info":[{"award-number":["WCU-58-001-HR"]}]}],"content-domain":{"domain":["wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2017,2]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>Heinz bodies are important to diagnosing and managing patients. However, microscopic examination of Heinz bodies has several disadvantages, demonstrating the need for a better method. We explored the potential use of flow cytometry to examine Heinz bodies.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Whole\u2010blood samples were collected from patients deficient in G6<jats:styled-content style=\"fixed-case\">PD<\/jats:styled-content> and healthy volunteers. Acetylphenylhydrazine was used to induce formation of Heinz bodies in red blood cells (<jats:styled-content style=\"fixed-case\">RBC<\/jats:styled-content>s). Then, <jats:styled-content style=\"fixed-case\">RBC<\/jats:styled-content>s positive for Heinz bodies were examined using a <jats:styled-content style=\"fixed-case\">FACSC<\/jats:styled-content>anto <jats:styled-content style=\"fixed-case\">II<\/jats:styled-content> cytometer.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">RBC<\/jats:styled-content>s treated with acetylphenylhydrazine formed Heinz bodies and emitted a broad spectrum of fluorescence that could be detected by flow cytometry. The maximum emission of fluorescence was observed at 45\u00a0min after the incubation with acetylphenylhydrazine. In addition, the fluorescence emitted was stable for at least 72\u00a0h. The flow cytometer could detect the <jats:styled-content style=\"fixed-case\">RBC<\/jats:styled-content>s positive for Heinz bodies even if they made up as little as 0.1% of the total <jats:styled-content style=\"fixed-case\">RBC<\/jats:styled-content> population. Furthermore, the percentage and number, respectively, of <jats:styled-content style=\"fixed-case\">RBC<\/jats:styled-content>s positive for Heinz bodies in G6<jats:styled-content style=\"fixed-case\">PD<\/jats:styled-content>\u2010deficient patients and normal donors exhibited a mean\u00a0\u00b1\u00a0standard deviation (<jats:styled-content style=\"fixed-case\">SD<\/jats:styled-content>) of 68.9\u00a0\u00b1\u00a027.5 <jats:italic>vs<\/jats:italic>. 50.9\u00a0\u00b1\u00a028.6 and 96\u00a0014\u00a0\u00b135\u00a0732 cells\/<jats:italic>\u03bc<\/jats:italic>L <jats:italic>vs<\/jats:italic>. 74\u00a0688\u00a0\u00b1\u00a036\u00a0514 cells\/<jats:italic>\u03bc<\/jats:italic>L.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Heinz bodies induced by acetylphenylhydrazine emit fluorescence, and this fluorescence could be examined using flow cytometry. Our study suggests the potential use of the developed method to investigate the formation of Heinz bodies in clinical samples.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijlh.12576","type":"journal-article","created":{"date-parts":[[2016,12,17]],"date-time":"2016-12-17T06:37:33Z","timestamp":1481956653000},"page":"68-75","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["A novel flow cytometry\u2010based method of analyzing Heinz bodies"],"prefix":"10.1111","volume":"39","author":[{"given":"D.","family":"Palasuwan","sequence":"first","affiliation":[{"name":"Department of Clinical Microscopy Faculty of Allied Health Sciences Chulalongkorn University Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1903-0512","authenticated-orcid":false,"given":"A.","family":"Palasuwan","sequence":"additional","affiliation":[{"name":"Department of Clinical Microscopy Faculty of 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