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Hb analysis was performed using the <jats:styled-content style=\"fixed-case\">cIEF<\/jats:styled-content> system. Thalassemia genotypes were defined by <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The system revealed satisfactorily within\u2010run and between\u2010run precision for quantitation of Hb A<jats:sub>2<\/jats:sub> and Hb E (<jats:styled-content style=\"fixed-case\">CV<\/jats:styled-content>: 0.02\u20130.09%). The reference ranges of Hb A<jats:sub>2<\/jats:sub> and Hb E were 2.6\u20134.0% and 25.7\u201333.1%, respectively. The system identified the cases of \u03b2\u2010thalassemia and Hb E disorders correctly. Several thalassemia genotypes and Hb variants were identifiable. However, Hb Constant Spring was separated closely to Hb A<jats:sub>2<\/jats:sub> and Hbs Bart's and H were relatively difficult to be reported due to interfering peaks separating at the same regions. Prenatal diagnosis by fetal blood analysis was found to be accurate for Hb Bart's hydrops fetalis and Hb E\u2010\u03b2<jats:sup>0<\/jats:sup>\u2010thalassemia disease.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The <jats:styled-content style=\"fixed-case\">cIEF<\/jats:styled-content> system could accurately diagnose \u03b2\u2010thalassemia and Hb E carriers and demonstrate many Hb variants found in the region. The system cannot report Hb A<jats:sub>2<\/jats:sub> in the presence of Hb E whereas Hbs Lepore and F are comigrated. Diagnosis of \u03b1\u2010thalassemia disease based on Hb H and Hb Bart's might be difficult.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijlh.12585","type":"journal-article","created":{"date-parts":[[2016,12,17]],"date-time":"2016-12-17T13:08:21Z","timestamp":1481980101000},"page":"101-111","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Diagnosis of common hemoglobinopathies among South East Asian population using capillary isoelectric focusing system"],"prefix":"10.1111","volume":"39","author":[{"given":"H.","family":"Srivorakun","sequence":"first","affiliation":[{"name":"Centre for Research and Development of Medical Diagnostic Laboratories Faculty of Associated Medical Sciences Khon Kaen University Khon Kaen Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"Fucharoen","sequence":"additional","affiliation":[{"name":"Centre for Research and Development of Medical Diagnostic Laboratories Faculty of Associated Medical Sciences Khon Kaen University Khon Kaen Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Sanchaisuriya","sequence":"additional","affiliation":[{"name":"Centre for Research and Development of Medical Diagnostic Laboratories Faculty of Associated Medical Sciences Khon Kaen University Khon Kaen Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Fucharoen","sequence":"additional","affiliation":[{"name":"Centre for Research and Development of Medical Diagnostic Laboratories Faculty of Associated Medical Sciences Khon Kaen University Khon Kaen Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,12,16]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.3109\/03630268709036587"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0108365"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/pd.2514"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-553X.2009.01158.x"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2004.034991"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1515\/CCLM.2006.059"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1309\/LMMH649POETQREXL"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1515\/CCLM.2003.114"},{"key":"e_1_2_5_10_1","doi-asserted-by":"crossref","first-page":"740","DOI":"10.1093\/clinchem\/44.4.740","article-title":"Prenatal and postnatal diagnoses of thalassemias and hemoglobinopathies by HPLC","volume":"44","author":"Fucharoen S","year":"1998","journal-title":"Clin Chem"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12098-007-0117-8"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0609.2009.01245.x"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trsl.2008.08.004"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-553X.2011.01301.x"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4347(97)00444-1"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0009-8981(99)00167-9"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1522-2683(20000301)21:4<743::AID-ELPS743>3.0.CO;2-1"},{"key":"e_1_2_5_18_1","doi-asserted-by":"crossref","first-page":"2137","DOI":"10.1093\/clinchem\/43.11.2137","article-title":"Capillary isoelectric focusing and high\u2010performance cation\u2010exchange chromatography compared for qualitative and quantitative analysis of hemoglobin variants","volume":"43","author":"Mario N","year":"1997","journal-title":"Clin Chem"},{"key":"e_1_2_5_19_1","first-page":"364","article-title":"A simplified screening strategy for thalassaemia and haemoglobin E in rural communities in South\u2010east Asia","volume":"82","author":"Fucharoen G","year":"2004","journal-title":"Bull World Health Organ"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00277-003-0689-y"},{"key":"e_1_2_5_21_1","unstructured":"The V8 system.V8 Operator Manual. 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