{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T23:45:21Z","timestamp":1774395921509,"version":"3.50.1"},"reference-count":16,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2017,4,19]],"date-time":"2017-04-19T00:00:00Z","timestamp":1492560000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2017,4,19]],"date-time":"2017-04-19T00:00:00Z","timestamp":1492560000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"},{"start":{"date-parts":[[2017,4,19]],"date-time":"2017-04-19T00:00:00Z","timestamp":1492560000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions"}],"content-domain":{"domain":["wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2017,8]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content> 200 is a type I immunoglobulin super family membrane glycoprotein, which is expressed in various mature B\u2010cell neoplasm (<jats:styled-content style=\"fixed-case\">MBN<\/jats:styled-content>). This study aimed at analyzing the expression pattern of <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>200 by flow cytometry immunophenotyping (<jats:styled-content style=\"fixed-case\">FCI<\/jats:styled-content>) and to evaluate its utility in narrowing down the differential diagnosis of <jats:styled-content style=\"fixed-case\">MBN<\/jats:styled-content>, particularly in low\u2010grade lymphomas.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A total of 160 samples were evaluated by <jats:styled-content style=\"fixed-case\">FCI<\/jats:styled-content> over a period of 2 years (July 2014\u2013June 2016), by a panel of antibodies including <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>200. The mean fluorescence intensity (<jats:styled-content style=\"fixed-case\">MFI<\/jats:styled-content>) of <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>200 in the neoplastic population was noted and compared among several groups of <jats:styled-content style=\"fixed-case\">MBN<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>All the 98 cases of chronic lymphocytic leukemia (<jats:styled-content style=\"fixed-case\">CLL<\/jats:styled-content>), five being <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>23\u2010negative, expressed <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>200 with moderate\u2010to\u2010bright intensity (median <jats:styled-content style=\"fixed-case\">MFI<\/jats:styled-content>: 1174). None of the 24 mantle cell lymphoma (<jats:styled-content style=\"fixed-case\">MCL<\/jats:styled-content>) cases, two being <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>23\u2010positive, expressed <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>200 (median <jats:styled-content style=\"fixed-case\">MFI<\/jats:styled-content>: 10). All six hairy cell leukemia (<jats:styled-content style=\"fixed-case\">HCL<\/jats:styled-content>) cases expressed <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>200. <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>200 expression in <jats:styled-content style=\"fixed-case\">HCL<\/jats:styled-content> was brightest among all the <jats:styled-content style=\"fixed-case\">MBN<\/jats:styled-content>s, with a median <jats:styled-content style=\"fixed-case\">MFI<\/jats:styled-content> of 5050. Other <jats:styled-content style=\"fixed-case\">MBN<\/jats:styled-content> (<jats:italic>n<\/jats:italic> = 32) expressed <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>200 in a proportion of cases and with variable intensity, usually dimmer than <jats:styled-content style=\"fixed-case\">CLL<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>200 has a valid role in differentiating <jats:styled-content style=\"fixed-case\">CLL<\/jats:styled-content> from <jats:styled-content style=\"fixed-case\">MCL<\/jats:styled-content>, especially in the cases with immunophenotypic overlap. <jats:styled-content style=\"fixed-case\">HCL<\/jats:styled-content> has a consistent and brightest expression of <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>200. It would be prudent to include <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>200 in the primary panel of antibodies for <jats:styled-content style=\"fixed-case\">MBN<\/jats:styled-content> analysis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijlh.12637","type":"journal-article","created":{"date-parts":[[2017,4,19]],"date-time":"2017-04-19T10:16:07Z","timestamp":1492596967000},"page":"384-391","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Role of <scp>CD<\/scp>200 in differential diagnosis of mature B\u2010cell neoplasm"],"prefix":"10.1111","volume":"39","author":[{"given":"K.","family":"Rahman","sequence":"first","affiliation":[{"name":"Department of Hematology SGPGI Lucknow India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"Kumar","sequence":"additional","affiliation":[{"name":"Department of Hematology SGPGI Lucknow India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Gupta","sequence":"additional","affiliation":[{"name":"Department of Hematology SGPGI Lucknow India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. K.","family":"Singh","sequence":"additional","affiliation":[{"name":"Department of Hematology SGPGI Lucknow India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Nityanand","sequence":"additional","affiliation":[{"name":"Department of Hematology SGPGI Lucknow India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,4,19]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00364281"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2567.2001.01163.x"},{"key":"e_1_2_6_4_1","first-page":"726","article-title":"CD 200 (OX\u20102 Membrane Glycoprotein) expression in B cell \u2013 derived neoplasms","volume":"134","author":"Dorfman DM","year":"2010","journal-title":"Am J ClinPathol"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.175.7.4441"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/1521-4141(200108)31:8<2331::AID-IMMU2331>3.0.CO;2-#"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.176.1.191"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2009.07644.x"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2404559"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2006-06-029355"},{"key":"e_1_2_6_11_1","first-page":"93","article-title":"Diagnostic usefulness and prognostic impact of CD 200 expression in lymphoid malignancies and plasma cell myeloma","volume":"137","author":"Alapat D","year":"2012","journal-title":"Am J ClinPathol"},{"key":"e_1_2_6_12_1","first-page":"536","article-title":"CD 200 flow cytometric assessment and semiquantitative immuno\u2010histochemical staining distinguishes hairy cell leukemia from hairy cell variant and other B \u2013 cell lymphoproliferative disorders","volume":"140","author":"Pillai V","year":"2013","journal-title":"Am J ClinPathol"},{"key":"e_1_2_6_13_1","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1002\/cytob.21128","article-title":"CD 200 has an important role in the differential diagnosis of mature B \u2013 cell neoplasms by multiparameter flow cytometry","volume":"86","author":"Sandes AF","year":"2014","journal-title":"Cytometry Part B (Clinical Cytometry)"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2007-11-120535"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.leukres.2009.01.017"},{"key":"e_1_2_6_16_1","first-page":"837","article-title":"Differential expression of CD 200 in B \u2013 cell neoplasms by flow cytometry can assist in diagnosis, sub classification and bone marrow staging","volume":"142","author":"Challagundla P","year":"2014","journal-title":"Am J ClinPathol"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.4103\/1110-1067.139754"}],"container-title":["International Journal of Laboratory Hematology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fijlh.12637","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ijlh.12637","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,23]],"date-time":"2023-09-23T19:44:59Z","timestamp":1695498299000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/ijlh.12637"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,4,19]]},"references-count":16,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2017,8]]}},"alternative-id":["10.1111\/ijlh.12637"],"URL":"https:\/\/doi.org\/10.1111\/ijlh.12637","archive":["Portico"],"relation":{},"ISSN":["1751-5521","1751-553X"],"issn-type":[{"value":"1751-5521","type":"print"},{"value":"1751-553X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,4,19]]}}}