{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T00:22:39Z","timestamp":1791591759661,"version":"4.3.4"},"reference-count":23,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2017,3,20]],"date-time":"2017-03-20T00:00:00Z","timestamp":1489968000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2017,3,20]],"date-time":"2017-03-20T00:00:00Z","timestamp":1489968000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"},{"start":{"date-parts":[[2017,3,20]],"date-time":"2017-03-20T00:00:00Z","timestamp":1489968000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions"}],"content-domain":{"domain":["wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2017,8]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>The t(6;9)(p23;q34);<jats:styled-content style=\"fixed-case\">DEK<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">NUP<\/jats:styled-content>214 [t(6;9)] abnormality is found in 0.7\u20131.8% of patients with acute myeloid leukemia (<jats:styled-content style=\"fixed-case\">AML<\/jats:styled-content>) or myelodysplastic syndromes (<jats:styled-content style=\"fixed-case\">MDS<\/jats:styled-content>). <jats:italic><jats:styled-content style=\"fixed-case\">FLT<\/jats:styled-content>3\u2010<\/jats:italic><jats:styled-content style=\"fixed-case\">ITD<\/jats:styled-content> mutations are detected in t(6;9) patients. The t(6;9) abnormality is associated with poor outcomes. We studied the clinicopathologic and molecular profiles of patients with <jats:styled-content style=\"fixed-case\">AML<\/jats:styled-content>\/<jats:styled-content style=\"fixed-case\">MDS<\/jats:styled-content> carrying t(6;9).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We collected clinical data of nine patients with <jats:styled-content style=\"fixed-case\">AML<\/jats:styled-content>\/<jats:styled-content style=\"fixed-case\">MDS<\/jats:styled-content> with isolated t(6;9) (median age\u00a0=\u00a041\u00a0years; male\/female\u00a0=\u00a04\/5) and genotyped <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>s using whole exome, Sanger, and targeted sequencing.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Our cohort was characterized by frequent multilineage dysplasia (56%), absence of phospho\u2010<jats:styled-content style=\"fixed-case\">STAT<\/jats:styled-content>3\/<jats:styled-content style=\"fixed-case\">STAT<\/jats:styled-content>5 expression, presence of myeloid markers (<jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>13, <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>33, <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>34, <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>117, <jats:styled-content style=\"fixed-case\">HLA<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">DR<\/jats:styled-content>) with an aberrant expression of <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>7, and poor outcome (median survival of 20\u00a0months). Although basophilia has been described in association with t(6;9), we observed lack of marrow basophilia in our cohort. Molecularly, 83% (5\/6) of patients with <jats:styled-content style=\"fixed-case\">AML<\/jats:styled-content>\/<jats:styled-content style=\"fixed-case\">MDS<\/jats:styled-content> with t(6;9) were characterized by at least one somatic mutation. Among them, four patients showed multiple mutations. <jats:italic><jats:styled-content style=\"fixed-case\">FLT<\/jats:styled-content>3\u2010<\/jats:italic><jats:styled-content style=\"fixed-case\">ITD<\/jats:styled-content>\u00a0mutations were detected in 33% of patients (2\/6); 80% (4\/5) of mutant patients died even after hematopoietic stem cell transplantation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our data demonstrated that <jats:styled-content style=\"fixed-case\">AML<\/jats:styled-content>\/<jats:styled-content style=\"fixed-case\">MDS<\/jats:styled-content> patients with t(6;9) have diverse molecular mutations regardless of the presence of <jats:italic><jats:styled-content style=\"fixed-case\">FLT<\/jats:styled-content><\/jats:italic>3 mutations, which may contribute to their poor survival outcomes.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijlh.12641","type":"journal-article","created":{"date-parts":[[2017,3,20]],"date-time":"2017-03-20T10:08:02Z","timestamp":1490004482000},"page":"409-417","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Clinicopathologic and molecular characterization of myeloid neoplasms with isolated t(6;9)(p23;q34)"],"prefix":"10.1111","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2993-1509","authenticated-orcid":false,"given":"V.","family":"Visconte","sequence":"first","affiliation":[{"name":"Department of Translational Hematology &amp; Oncology Research Cleveland Clinic Cleveland OH USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Shetty","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Cleveland Clinic Cleveland OH USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"Przychodzen","sequence":"additional","affiliation":[{"name":"Department of Translational Hematology &amp; Oncology Research Cleveland Clinic Cleveland OH USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Hirsch","sequence":"additional","affiliation":[{"name":"Department of Translational Hematology &amp; Oncology Research Cleveland Clinic Cleveland OH USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Bodo","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Cleveland Clinic Cleveland OH USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. P.","family":"Maciejewski","sequence":"additional","affiliation":[{"name":"Department of Translational Hematology &amp; Oncology Research Cleveland Clinic Cleveland OH USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8623-4067","authenticated-orcid":false,"given":"E. D.","family":"Hsi","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Cleveland Clinic Cleveland OH USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H. J.","family":"Rogers","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Cleveland Clinic Cleveland OH USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,3,20]]},"reference":[{"key":"e_1_2_8_2_1","first-page":"115","volume-title":"WHO Classification of Tumours of Haematopoietic and Lymphoid Tissues","author":"Arber D","year":"2008"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-4608(93)90119-7"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-4608(89)90089-7"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/107.4.430"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2009-11-254441"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2009.24.8997"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjh.12852"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2011.229"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2007-05-091215"},{"key":"e_1_2_8_11_1","volume-title":"An International System for Human Cytogenetic Nomenclature","author":"Shaffer LG","year":"2009"},{"key":"e_1_2_8_12_1","first-page":"527","article-title":"Translocation t(6;9)(p23;q34) in acute myeloid leukemia without myelodysplasia or basophilia: two cases and a review of the literature","volume":"7","author":"Lillington DM","year":"1993","journal-title":"Leukemia"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.18632\/genesandcancer.39"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2009-06-227603"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V99.12.4326"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2013.098517"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2404233"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2011.350"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1301689"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1516192"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2014.243"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2012-04-426965"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11010-013-1704-9"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2005-03-0867"}],"container-title":["International Journal of Laboratory Hematology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fijlh.12641","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ijlh.12641","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,23]],"date-time":"2023-09-23T19:45:13Z","timestamp":1695498313000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/ijlh.12641"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,3,20]]},"references-count":23,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2017,8]]}},"alternative-id":["10.1111\/ijlh.12641"],"URL":"https:\/\/doi.org\/10.1111\/ijlh.12641","archive":["Portico"],"relation":{},"ISSN":["1751-5521","1751-553X"],"issn-type":[{"value":"1751-5521","type":"print"},{"value":"1751-553X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,3,20]]}}}