{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T21:30:53Z","timestamp":1789421453911,"version":"build-2803163510"},"reference-count":16,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2017,5,12]],"date-time":"2017-05-12T00:00:00Z","timestamp":1494547200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2017,5,12]],"date-time":"2017-05-12T00:00:00Z","timestamp":1494547200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"},{"start":{"date-parts":[[2017,5,12]],"date-time":"2017-05-12T00:00:00Z","timestamp":1494547200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2017,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>It has been recently suggested that microparticles (<jats:styled-content style=\"fixed-case\">MP<\/jats:styled-content>) play a role in the pathogenesis of thrombotic complications. This study aimed to assess the contribution of procoagulant activity expressed by circulating <jats:styled-content style=\"fixed-case\">MP<\/jats:styled-content> in thrombotic events in <jats:styled-content style=\"fixed-case\">MPN<\/jats:styled-content> patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Seventy\u2010four <jats:styled-content style=\"fixed-case\">MPN<\/jats:styled-content> patients were enrolled in a trans\u2010sectional study. The <jats:styled-content style=\"fixed-case\">MP<\/jats:styled-content> procoagulant activity was measured using two assays: (i) the thrombin generation (TG) assay used in different conditions with the addition of both tissue factor (TF) and phospholipids (PL) and with the addition of TF or PL alone and (ii) the <jats:styled-content style=\"fixed-case\">PROCOAG<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PPL<\/jats:styled-content> assay.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The mean age was 62 (26 men and 48 women). The prevalence of thrombotic events was 28%. When comparing patients with thrombosis to those without, age, sex, <jats:styled-content style=\"fixed-case\">MPN<\/jats:styled-content> type, cardiovascular risk factors, and history of thrombosis were not significantly associated with thrombosis. The <jats:styled-content style=\"fixed-case\">JAK<\/jats:styled-content>2 V617F mutation was significantly associated with thrombotic events (90% vs 67%; <jats:italic>P<\/jats:italic>=.04). Results from the TG assay and the <jats:styled-content style=\"fixed-case\">PROCOAG<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PPL<\/jats:styled-content> assays did not demonstrate a significant association between the <jats:styled-content style=\"fixed-case\">MP<\/jats:styled-content> procoagulant activity and thrombotic events.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The <jats:styled-content style=\"fixed-case\">MP<\/jats:styled-content> procoagulant activity did not predict thrombosis in <jats:styled-content style=\"fixed-case\">MPN<\/jats:styled-content> patients. The contribution of TG assay in the assessment of the thrombotic risk is still in debate.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijlh.12676","type":"journal-article","created":{"date-parts":[[2017,5,12]],"date-time":"2017-05-12T06:00:47Z","timestamp":1494568847000},"page":"502-507","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["The evaluation of the relevance of thrombin generation and procoagulant activity in thrombotic risk assessment in <scp>BCR<\/scp>\u2010<scp>ABL<\/scp>\u2010negative myeloproliferative neoplasm patients"],"prefix":"10.1111","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6995-2435","authenticated-orcid":false,"given":"H.","family":"Baccouche","sequence":"first","affiliation":[{"name":"Hematology department\u2010Rabta University Hospital Faculty of Medicine of Tunis Tunis University El Manar  Tunis 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Medicine of Tunis Tunis University El Manar  Tunis Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I.","family":"Sfar","sequence":"additional","affiliation":[{"name":"Immunology department\u2010Charles Nicolle University Hospital Faculty of Medicine of Tunis Tunis University El Manar  Tunis Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.","family":"Gorgi","sequence":"additional","affiliation":[{"name":"Immunology department\u2010Charles Nicolle University Hospital Faculty of Medicine of Tunis Tunis University El Manar  Tunis Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N.","family":"Ben Romdhane","sequence":"additional","affiliation":[{"name":"Hematology department\u2010Rabta University Hospital Faculty of Medicine of Tunis Tunis University El Manar  Tunis 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