{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T00:56:34Z","timestamp":1792457794717,"version":"4.5.0"},"reference-count":22,"publisher":"Wiley","issue":"S1","license":[{"start":{"date-parts":[[2017,4,26]],"date-time":"2017-04-26T00:00:00Z","timestamp":1493164800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2017,4,26]],"date-time":"2017-04-26T00:00:00Z","timestamp":1493164800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1"},{"start":{"date-parts":[[2017,4,26]],"date-time":"2017-04-26T00:00:00Z","timestamp":1493164800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions"}],"content-domain":{"domain":["wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2017,5]]},"abstract":"Summary<\/jats:title>We have developed a lymphoproliferative disorder screening tube (LPD<\/jats:styled-content>\u2010ST<\/jats:styled-content>) with the aim to provide comprehensive immunophenotyping of lymphocyte subsets with minimal need for additional testing. The LPD<\/jats:styled-content>\u2010ST<\/jats:styled-content> consists of CD4\/kappa FITC<\/jats:styled-content>, CD8\/lambda PE<\/jats:styled-content>, CD<\/jats:styled-content>3\/CD<\/jats:styled-content>14ECD<\/jats:styled-content>, CD<\/jats:styled-content>38PC<\/jats:styled-content>5.5, CD<\/jats:styled-content>20\/CD<\/jats:styled-content>56PC<\/jats:styled-content>7, CD<\/jats:styled-content>10APC<\/jats:styled-content>, CD<\/jats:styled-content>19APC<\/jats:styled-content>\u2010A700, CD<\/jats:styled-content>5APC<\/jats:styled-content>\u2010A750, CD<\/jats:styled-content>57\/CD<\/jats:styled-content>23PB<\/jats:styled-content> and CD<\/jats:styled-content>45KO<\/jats:styled-content>. The LPD<\/jats:styled-content>\u2010ST<\/jats:styled-content> was validated against previously used lymphocyte subset panels in Canada (n=60) and in Sweden (n=43) and against the OneFlow\u2122<\/jats:sup> LST<\/jats:styled-content> (n=60). The LPD<\/jats:styled-content>\u2010ST<\/jats:styled-content> panel was then implemented in clinical practice using dried monoclonal antibody reagents (Duraclone\u00ae<\/jats:sup>) on 649 patient samples in Sweden. In 204 of 649 samples (31%), a monotypic B\u2010cell population was found. Of these cases, a final diagnosis could be rendered in 106 cases (52%), and in the remainder, additional B\u2010cell immunophenotyping was performed. In 20 (3%) samples, an aberrant T\u2010cell population was confirmed by additional testing. Of 425 samples diagnosed as normal\/reactive lymphoid tissue, 50 (12%) required additional immunophenotyping, mostly due to an abnormal CD<\/jats:styled-content>4\/CD<\/jats:styled-content>8 ratio. The LPD<\/jats:styled-content>\u2010ST<\/jats:styled-content> tube significantly minimizes the need for additional testing, improves the turn\u2010around time, and reduces the cost of LPD<\/jats:styled-content> immunophenotyping. It is also suitable for investigating paucicellular samples such as cerebrospinal fluid or fine needle aspirates.<\/jats:p>","DOI":"10.1111\/ijlh.12678","type":"journal-article","created":{"date-parts":[[2017,4,27]],"date-time":"2017-04-27T01:40:49Z","timestamp":1493257249000},"page":"76-85","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["Ten\u2010color 15\u2010antibody flow cytometry panel for immunophenotyping of lymphocyte population"],"prefix":"10.1111","volume":"39","author":[{"given":"A.","family":"Rajab","sequence":"first","affiliation":[{"name":"Hematology Department LifeLabs Toronto ON Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"O.","family":"Axler","sequence":"additional","affiliation":[{"name":"Klinisk patologi, Labmedicin Medicinsk Service, Region Sk\u00e5ne Lunds Universitetsjukhus Lund Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Leung","sequence":"additional","affiliation":[{"name":"Flow Cytometry Laboratory Laboratory Medicine Program University Health Network Toronto ON Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Wozniak","sequence":"additional","affiliation":[{"name":"Hematology Department LifeLabs Toronto ON Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Porwit","sequence":"additional","affiliation":[{"name":"Division for Oncology and Pathology Department of Clinical Sciences Lund Faculty of Medicine Lund University Lund Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,4,26]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/ijlh.12080"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21402"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21233"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2008.17.7089"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.a.22056"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2404241"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2012.120"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21251"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2016.44"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.20365"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.20363"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2010.312"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2007-11-120535"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjh.12789"},{"key":"e_1_2_7_16_1","volume-title":"Ten\u2010Color 15 Antibody Flow Cytometry Panel for Immunophenotyping of Lymphocyte population","author":"Rajab A","year":"2015"},{"key":"e_1_2_7_17_1","volume-title":"WHO Classification of Tumours of Haematopoietic and Lymphoid Tissues","author":"Swerdlow SH","year":"2008"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2012.122"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2015.313"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.3109\/10428194.2016.1170827"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1309\/UQ4N-M5KL-0ANY-YD3G"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2010-07-296962"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1097\/MOH.0b013e328340dc12"}],"container-title":["International Journal of Laboratory Hematology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fijlh.12678","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ijlh.12678","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,12]],"date-time":"2023-09-12T06:52:32Z","timestamp":1694501552000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/ijlh.12678"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,4,26]]},"references-count":22,"journal-issue":{"issue":"S1","published-print":{"date-parts":[[2017,5]]}},"alternative-id":["10.1111\/ijlh.12678"],"URL":"https:\/\/doi.org\/10.1111\/ijlh.12678","archive":["Portico"],"relation":{},"ISSN":["1751-5521","1751-553X"],"issn-type":[{"value":"1751-5521","type":"print"},{"value":"1751-553X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,4,26]]}}}