{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,3]],"date-time":"2026-03-03T01:58:16Z","timestamp":1772503096914,"version":"3.50.1"},"reference-count":40,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2017,10,8]],"date-time":"2017-10-08T00:00:00Z","timestamp":1507420800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2017,10,8]],"date-time":"2017-10-08T00:00:00Z","timestamp":1507420800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"},{"start":{"date-parts":[[2017,10,8]],"date-time":"2017-10-08T00:00:00Z","timestamp":1507420800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2017,12]]},"abstract":"Abstract<\/jats:title>Introduction<\/jats:title>Recent automated hematology analyzers (HA<\/jats:styled-content>s) can identify and report nucleated red blood cells (NRBC<\/jats:styled-content>) count as a separate population out of white blood cells (WBC<\/jats:styled-content>). The aim of this study was to investigate the analytical performances of NRBC<\/jats:styled-content> enumeration on five top of the range HA<\/jats:styled-content>s.<\/jats:p><\/jats:sec>Methods<\/jats:title>We evaluated the within\u2010run and between\u2010day precision, limit of blank (LoB), limit of detection (LoD), and limit of quantitation (LoQ) of XE<\/jats:styled-content>\u20102100 and XN<\/jats:styled-content>\u2010module (Sysmex), ADVIA<\/jats:styled-content> 2120i (Siemens), BC<\/jats:styled-content>\u20106800 (Mindray), and UniCel DxH 800 (Beckman Coulter). Automated NRBC<\/jats:styled-content> counts were also compared with optical microscopy (OM<\/jats:styled-content>).<\/jats:p><\/jats:sec>Results<\/jats:title>The limits of detection for NRBC<\/jats:styled-content> of the BC<\/jats:styled-content>\u20106800, XN<\/jats:styled-content>\u2010module, XE<\/jats:styled-content>\u20102100, UniCel DxH 800, and ADVIA<\/jats:styled-content> 2120i are 0.035\u00d7109<\/jats:sup>\/L, 0.019\u00d7109<\/jats:sup>\/L, 0.067\u00d7109<\/jats:sup>\/L, 0.038\u00d7109<\/jats:sup>\/L, and 0.167\u00d7109<\/jats:sup>\/L, respectively. Our data indicated excellent performance in terms of precision. The agreement with OM<\/jats:styled-content> was excellent for BC<\/jats:styled-content>\u20106800, XN<\/jats:styled-content>\u2010module, and XE<\/jats:styled-content>\u20102100 (Bias 0.023, 0.019, and 0.033\u00d7109<\/jats:sup>\/L, respectively). ADVIA<\/jats:styled-content> 2120i displayed a significant constant error and UniCel DxH 800 both proportional and small constant error.<\/jats:p><\/jats:sec>Conclusion<\/jats:title>Regards to NRBC<\/jats:styled-content> counting, the performances shown by BC<\/jats:styled-content>\u20106800, XN<\/jats:styled-content>\u2010module, and XE<\/jats:styled-content>\u20102100 are excellent also a low count, ADVIA<\/jats:styled-content> 2120i and UniCel DxH 800 need to be improved.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijlh.12722","type":"journal-article","created":{"date-parts":[[2017,10,9]],"date-time":"2017-10-09T04:26:40Z","timestamp":1507523200000},"page":"663-670","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Performance evaluation of the automated nucleated red blood cell count of five commercial hematological analyzers"],"prefix":"10.1111","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9339-4066","authenticated-orcid":false,"given":"G.","family":"Da Rin","sequence":"first","affiliation":[{"name":"Laboratory Medicine San Bassiano Hospital ASL 7 Pedemontana Bassano del Grappa Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Vidali","sequence":"additional","affiliation":[{"name":"Immunohematology and Transfusion Medicine Service Hospital SS. Trinit\u00e0 Borgomanero Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F.","family":"Balboni","sequence":"additional","affiliation":[{"name":"Laboratory Medicine Istituto Fiorentino di Cura e Assistenza (IFCA) Firenze Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Benegiamo","sequence":"additional","affiliation":[{"name":"Laboratory of Clinical Chemistry and Hematology Hospital of Parma Parma Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Borin","sequence":"additional","affiliation":[{"name":"Clinical Chemistry Laboratory and\u00a0 Microbiology ASL NO Borgomanero Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. L.","family":"Ciardelli","sequence":"additional","affiliation":[{"name":"Clinical Chemistry Laboratory IRCCS Policlinico San Matteo Pavia Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F.","family":"Dima","sequence":"additional","affiliation":[{"name":"Clinical Chemistry Laboratory AOUI Verona Verona Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Di Fabio","sequence":"additional","affiliation":[{"name":"Clinical Chemistry Laboratory Hospital Civile Avezzano Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Fanelli","sequence":"additional","affiliation":[{"name":"General Laboratory University Hospital Careggi Firenze Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F.","family":"Fiorini","sequence":"additional","affiliation":[{"name":"Clinical Pathology Laboratory USL Nordovest Toscana Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Francione","sequence":"additional","affiliation":[{"name":"Clinical Chemistry Laboratory and\u00a0 Microbiology ASL NO Borgomanero Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1456-2974","authenticated-orcid":false,"given":"L.","family":"Germagnoli","sequence":"additional","affiliation":[{"name":"SynLab Cornaredolo Cornaredolo Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Gioia","sequence":"additional","affiliation":[{"name":"Clinical Chemistry Laboratory Hospital Abele Ajello Mazara del Vallo Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.","family":"Lari","sequence":"additional","affiliation":[{"name":"Clinical Chemistry Laboratory Hospital Versilia Azienda Toscana Nord ovest Lido di Camaiore Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Lorubbio","sequence":"additional","affiliation":[{"name":"Laboratory Medicine Fondazione G. 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