{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T18:18:00Z","timestamp":1790360280018,"version":"4.1.0"},"reference-count":28,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,9,3]],"date-time":"2017-09-03T00:00:00Z","timestamp":1504396800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2017,9,3]],"date-time":"2017-09-03T00:00:00Z","timestamp":1504396800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"},{"start":{"date-parts":[[2017,9,3]],"date-time":"2017-09-03T00:00:00Z","timestamp":1504396800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2018,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>Cellular analysis in cerebrospinal fluid (<jats:styled-content style=\"fixed-case\">CSF<\/jats:styled-content>) provides important diagnostic information in many pathological settings. The aim of this two\u2010site study was to evaluate the Sysmex <jats:styled-content style=\"fixed-case\">XN<\/jats:styled-content> Body Fluid mode (<jats:styled-content style=\"fixed-case\">XN<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">BF<\/jats:styled-content>) for cell analysis of <jats:styled-content style=\"fixed-case\">CSF<\/jats:styled-content> compared to light microscopy (<jats:styled-content style=\"fixed-case\">LM<\/jats:styled-content>).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Two hundred and seven consecutive <jats:styled-content style=\"fixed-case\">CSF<\/jats:styled-content> samples were analyzed in parallel with <jats:styled-content style=\"fixed-case\">XN<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">BF<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">LM<\/jats:styled-content>. The study also included the estimation of the limit of blank (LoB), limit of detection (LoD), limit of quantitation (LoQ), carry\u2010over and linearity of <jats:styled-content style=\"fixed-case\">XN<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">BF<\/jats:styled-content> module.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>LoQ of white blood cells (<jats:styled-content style=\"fixed-case\">WBC<\/jats:styled-content>) was 3\u00d710<jats:sup>6<\/jats:sup>\u00a0cells\/L; linearity was good and carry\u2010over negligible. <jats:styled-content style=\"fixed-case\">XN<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">BF<\/jats:styled-content> parameters were compared to <jats:styled-content style=\"fixed-case\">LM<\/jats:styled-content> for the following cell classes: total cells, <jats:styled-content style=\"fixed-case\">WBC<\/jats:styled-content>, polymorphonuclear (<jats:styled-content style=\"fixed-case\">PMN<\/jats:styled-content>), and mononuclear (<jats:styled-content style=\"fixed-case\">MN<\/jats:styled-content>) cells. The bias ranged from 1.3 to 15.2\u00d710<jats:sup>6<\/jats:sup><jats:bold>\u00a0<\/jats:bold>cells\/L. The receiver operating characteristics curve analysis for <jats:styled-content style=\"fixed-case\">WBC<\/jats:styled-content> showed an area under the curve of 0.98, and the global diagnostic agreement was 95% at a cutoff of 5\u00d710<jats:sup>6<\/jats:sup><jats:bold>\u00a0<\/jats:bold>cells\/L.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">XN<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">BF<\/jats:styled-content> provides rapid and accurate counts in clinically relevant ranges of <jats:styled-content style=\"fixed-case\">CSF<\/jats:styled-content> values, thus providing a valuable alternative to conventional <jats:styled-content style=\"fixed-case\">LM<\/jats:styled-content> analysis. However, microscopic review remains advisable in samples with abnormal cell counts or high fluorescent (<jats:styled-content style=\"fixed-case\">HF<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">BF<\/jats:styled-content>) cell parameter exceeding 5\u00d710<jats:sup>6<\/jats:sup><jats:bold>\u00a0<\/jats:bold>cells\/L.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijlh.12723","type":"journal-article","created":{"date-parts":[[2017,9,3]],"date-time":"2017-09-03T12:22:36Z","timestamp":1504441356000},"page":"26-33","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Two\u2010site evaluation of the diagnostic performance of the Sysmex <scp>XN<\/scp> Body Fluid (<scp>BF<\/scp>) module for cell count and differential in Cerebrospinal 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