{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T05:31:40Z","timestamp":1785216700282,"version":"3.55.0"},"reference-count":38,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2017,9,30]],"date-time":"2017-09-30T00:00:00Z","timestamp":1506729600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2017,9,30]],"date-time":"2017-09-30T00:00:00Z","timestamp":1506729600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"},{"start":{"date-parts":[[2017,9,30]],"date-time":"2017-09-30T00:00:00Z","timestamp":1506729600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Centre of Postgraduate Medical Education, Warsaw, Poland","award":["501-1-26-02-16"],"award-info":[{"award-number":["501-1-26-02-16"]}]}],"content-domain":{"domain":["wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2018,4]]},"abstract":"Abstract<\/jats:title>Introduction<\/jats:title>Hereditary haemolytic anaemias (HHA<\/jats:styled-content>) encompass a heterogeneous group of anaemias characterized by decreased red blood cell survival. The aim of this study was to evaluate the status of red blood cell (RBC<\/jats:styled-content>) surface molecules known or previously proposed to participate in preventing premature RBC<\/jats:styled-content> clearance, analysing erythrocytes from patients with two types of HHA<\/jats:styled-content>: hereditary spherocytosis (HS<\/jats:styled-content>) and microcytosis.<\/jats:p><\/jats:sec>Material\/Methods<\/jats:title>Relative binding of five monoclonal antibodies (mA<\/jats:styled-content>bs), anti\u2010CD<\/jats:styled-content>55, anti\u2010CD<\/jats:styled-content>59, anti\u2010CD<\/jats:styled-content>44, anti\u2010CD<\/jats:styled-content>47 and anti\u2010CD<\/jats:styled-content>58, was evaluated in erythrocytes of patients with HS<\/jats:styled-content> and hereditary microcytosis, using flow cytometry. The amount of CD<\/jats:styled-content>55 protein was assessed by semi\u2010quantitative Western blots densitometry analysis.<\/jats:p><\/jats:sec>Results<\/jats:title>The majority of both HS<\/jats:styled-content> and microcytic patients demonstrated significant reduction of anti\u2010CD<\/jats:styled-content>55 binding by erythrocytes (average 23% and 19%, respectively, P\u00a0<\/jats:italic><\u00a0<\/jats:italic>.001), with no concomitant anti\u2010CD<\/jats:styled-content>59\u2010binding deficiency. Anti\u2010CD<\/jats:styled-content>44, anti\u2010CD<\/jats:styled-content>47 and anti\u2010CD<\/jats:styled-content>58 binding was within the healthy control range or was slightly decreased.<\/jats:p><\/jats:sec>Conclusions<\/jats:title>This study provides evidence supporting the presence of erythrocytes deficient in CD<\/jats:styled-content>55 presentation in HS<\/jats:styled-content> and hereditary microcytosis. Moreover, deficiency of CD<\/jats:styled-content>55 antigen presentation on RBC<\/jats:styled-content> does not correlate with the amount of CD<\/jats:styled-content>55 in RBC<\/jats:styled-content> membrane. Further studies using molecular techniques will clarify the exact participation of CD<\/jats:styled-content>55 deficiency in premature RBC<\/jats:styled-content> clearance in HHA<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijlh.12752","type":"journal-article","created":{"date-parts":[[2017,9,30]],"date-time":"2017-09-30T05:56:45Z","timestamp":1506751005000},"page":"128-135","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Diminished presentation of complement regulatory protein CD<\/scp>55 on red blood cells from patients with hereditary haemolytic anaemias"],"prefix":"10.1111","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1423-0168","authenticated-orcid":false,"given":"A.","family":"Loniewska\u2010Lwowska","sequence":"first","affiliation":[{"name":"Department of Immunohaematology Centre of Postgraduate Medical Education Warsaw Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Koza","sequence":"additional","affiliation":[{"name":"Department of Immunohaematology Centre of Postgraduate Medical Education Warsaw Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Mendek\u2010Czajkowska","sequence":"additional","affiliation":[{"name":"Outpatient Clinic for Congenital Anaemias Institute of Haematology and Blood Transfusion Warsaw Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Wieszczy","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology and Clinical Oncology Centre of Postgraduate Medical Education Warsaw Poland"},{"name":"Department of Cancer Prevention The Maria Sk\u0142odowska\u2010Curie Memorial Cancer Centre and Institute of Oncology Warsaw Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Adamowicz\u2010Salach","sequence":"additional","affiliation":[{"name":"Department of Paediatrics, Haematology and Oncology Medical University of Warsaw Warsaw Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Branicka","sequence":"additional","affiliation":[{"name":"Department of Immunohaematology Centre of Postgraduate Medical Education Warsaw Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I.","family":"Witos","sequence":"additional","affiliation":[{"name":"Department of Immunohaematology Centre of Postgraduate Medical Education Warsaw Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Sapala\u2010Smoczynska","sequence":"additional","affiliation":[{"name":"Department of Paediatrics Medical Centre of Postgraduate Education Bielanski Hospital Warsaw Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Jackowska","sequence":"additional","affiliation":[{"name":"Department of Paediatrics Medical Centre of Postgraduate Education Bielanski Hospital Warsaw Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Fabijanska\u2010Mitek","sequence":"additional","affiliation":[{"name":"Department of Immunohaematology Centre of Postgraduate Medical Education Warsaw Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,9,30]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.seminhematol.2004.01.002"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.13619"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12013-009-9056-5"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1159\/000086406"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1159\/000315102"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.288.5473.2051"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2011-10-386805"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1471-4914(01)01963-3"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1006\/jaut.2001.0563"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.transci.2005.09.006"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exphem.2007.08.029"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.3233\/CH-2010-1253"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2007.01549.x"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2013.00365"},{"key":"e_1_2_7_16_1","volume-title":"Haemoglobin Concentrations for the Diagnosis of Anaemia and Assessment of Severity. 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