{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T05:38:02Z","timestamp":1790401082048,"version":"4.1.0"},"reference-count":26,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2017,12,4]],"date-time":"2017-12-04T00:00:00Z","timestamp":1512345600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2017,12,4]],"date-time":"2017-12-04T00:00:00Z","timestamp":1512345600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"},{"start":{"date-parts":[[2017,12,4]],"date-time":"2017-12-04T00:00:00Z","timestamp":1512345600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003407","name":"Ministero dell\u2019Istruzione, dell\u2019Universit\u00e0 e della Ricerca","doi-asserted-by":"publisher","award":["7.07.02.60 AE01"],"award-info":[{"award-number":["7.07.02.60 AE01"]}],"id":[{"id":"10.13039\/501100003407","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007364","name":"Fondazione CRT","doi-asserted-by":"publisher","award":["2015.1044"],"award-info":[{"award-number":["2015.1044"]}],"id":[{"id":"10.13039\/100007364","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007364","name":"Fondazione CRT","doi-asserted-by":"publisher","award":["2016.0677"],"award-info":[{"award-number":["2016.0677"]}],"id":[{"id":"10.13039\/100007364","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Progetto di Rilevante Interesse Nazionale","award":["PRIN2009"],"award-info":[{"award-number":["PRIN2009"]}]},{"DOI":"10.13039\/501100003407","name":"Ministero dell\u2019Istruzione, dell\u2019Universit\u00e0 e della Ricerca","doi-asserted-by":"publisher","award":["7.07.02.60 AE01"],"award-info":[{"award-number":["7.07.02.60 AE01"]}],"id":[{"id":"10.13039\/501100003407","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Progetto di Ricerca Sanitaria Finalizzata","award":["RF-2009-1469205"],"award-info":[{"award-number":["RF-2009-1469205"]}]},{"name":"Progetto di Ricerca Sanitaria Finalizzata","award":["RF-2010-2307262"],"award-info":[{"award-number":["RF-2010-2307262"]}]},{"name":"Fondazione Neoplasie Del Sangue"},{"DOI":"10.13039\/100007364","name":"Fondazione CRT","doi-asserted-by":"publisher","award":["2015.1044"],"award-info":[{"award-number":["2015.1044"]}],"id":[{"id":"10.13039\/100007364","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007364","name":"Fondazione CRT","doi-asserted-by":"publisher","award":["2016.0677"],"award-info":[{"award-number":["2016.0677"]}],"id":[{"id":"10.13039\/100007364","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Associazione DaRosa"}],"content-domain":{"domain":["wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2018,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>The high\u2010throughput era remarkably changed molecular laboratory practice. Actually, the increasing number of processed samples requires to reduce the risk of operator biases, by automating or simplifying as much as possible both the analytical and the pre\u2010analytical phases. Minimal residual disease (<jats:styled-content style=\"fixed-case\">MRD<\/jats:styled-content>) studies in hematology often require a simultaneous processing of many bone marrow and peripheral blood samples from patients enrolled in prospective, multicenter, clinical trials, monitored at several planned time points.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>In this study, we demonstrate that red blood cell lysis (<jats:styled-content style=\"fixed-case\">RBL<\/jats:styled-content>) pre\u2010analytical procedure can replace the time\u2010consuming Ficoll stratification as cell recovering step. Here, we show a <jats:styled-content style=\"fixed-case\">MRD<\/jats:styled-content> comparison study using both total white blood cells and mononuclear cells recovered by the 2 procedures from 46 follicular lymphoma (<jats:styled-content style=\"fixed-case\">FL<\/jats:styled-content>), 15 multiple myeloma (<jats:styled-content style=\"fixed-case\">MM<\/jats:styled-content>), and 11 mantle cell lymphoma (<jats:styled-content style=\"fixed-case\">MCL<\/jats:styled-content>) patients enrolled in prospective clinical trials.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The experiments were performed in the 4 laboratories of the Fondazione Italiana Linfomi (<jats:styled-content style=\"fixed-case\">FIL<\/jats:styled-content>) <jats:styled-content style=\"fixed-case\">MRD<\/jats:styled-content> Network and showed superimposable results, in terms of good correlation (<jats:italic>R\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>0.87) of the <jats:styled-content style=\"fixed-case\">MRD<\/jats:styled-content> data obtained by recovering blood cells by the 2 approaches.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Based on these results, the <jats:styled-content style=\"fixed-case\">FIL MRD<\/jats:styled-content> Network suggests to optimize the pre\u2010analytical phases introducing <jats:styled-content style=\"fixed-case\">RBL<\/jats:styled-content> approach for cell recovery in the clinical trials including <jats:styled-content style=\"fixed-case\">MRD<\/jats:styled-content> analysis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijlh.12766","type":"journal-article","created":{"date-parts":[[2017,12,5]],"date-time":"2017-12-05T10:33:01Z","timestamp":1512469981000},"page":"201-208","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Ficoll\u2010hypaque separation vs whole blood lysis: Comparison of efficiency and impact on minimal residual disease analysis"],"prefix":"10.1111","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9624-0337","authenticated-orcid":false,"given":"E.","family":"Genuardi","sequence":"first","affiliation":[{"name":"Department of Molecular Biotechnologies and Health Sciences Division of Hematology University of Torino  Torino Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Barbero","sequence":"additional","affiliation":[{"name":"Department of Molecular Biotechnologies and Health Sciences Division of Hematology University of Torino  Torino Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I.","family":"Dogliotti","sequence":"additional","affiliation":[{"name":"Department of Molecular Biotechnologies and Health Sciences Division of Hematology University of Torino  Torino Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"Mantoan","sequence":"additional","affiliation":[{"name":"Department of Molecular Biotechnologies and Health Sciences Division of Hematology University of Torino  Torino Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Drandi","sequence":"additional","affiliation":[{"name":"Department of Molecular Biotechnologies and Health Sciences Division of Hematology University of Torino  Torino Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Gambella","sequence":"additional","affiliation":[{"name":"Department of Molecular Biotechnologies and Health Sciences Division of Hematology University of Torino  Torino Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G. M.","family":"Zaccaria","sequence":"additional","affiliation":[{"name":"Department of Molecular Biotechnologies and Health Sciences Division of Hematology University of Torino  Torino Italy"},{"name":"Division of Hematology Department of Cellular Biotechnologies and Hematology \u201cSapienza\u201d University of Rome  Rome Italy"},{"name":"Division of Hematology Department of Oncology Santa Chiara Hospital  Pisa Italy"},{"name":"Department of Medical Biotechnologies University of Siena  Siena Italy"},{"name":"Hematopathology Section Department of Experimental, Diagnostic, and Specialty Medicine S. Orsola\u2010Malpighi Hospital Bologna University  Bologna Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Monitillo","sequence":"additional","affiliation":[{"name":"Department of Molecular Biotechnologies and Health Sciences Division of Hematology University of Torino  Torino Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I.","family":"Della Starza","sequence":"additional","affiliation":[{"name":"Division of Hematology Department of Cellular Biotechnologies and Hematology \u201cSapienza\u201d University of Rome  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2773-5483","authenticated-orcid":false,"given":"M.","family":"Cavalli","sequence":"additional","affiliation":[{"name":"Division of Hematology Department of Cellular Biotechnologies and Hematology \u201cSapienza\u201d University of Rome  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L. A.","family":"De Novi","sequence":"additional","affiliation":[{"name":"Division of Hematology Department of Cellular Biotechnologies and Hematology \u201cSapienza\u201d University of Rome  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Ciabatti","sequence":"additional","affiliation":[{"name":"Division of Hematology Department of Oncology Santa Chiara Hospital  Pisa Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Grassi","sequence":"additional","affiliation":[{"name":"Division of Hematology Department of Oncology Santa Chiara Hospital  Pisa Italy"},{"name":"Department of Medical Biotechnologies University of Siena  Siena Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Gazzola","sequence":"additional","affiliation":[{"name":"Hematopathology Section Department of Experimental, Diagnostic, and Specialty Medicine S. Orsola\u2010Malpighi Hospital Bologna University  Bologna Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Mannu","sequence":"additional","affiliation":[{"name":"Hematopathology Section Department of Experimental, Diagnostic, and Specialty Medicine S. Orsola\u2010Malpighi Hospital Bologna University  Bologna Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I.","family":"Del Giudice","sequence":"additional","affiliation":[{"name":"Division of Hematology Department of Cellular Biotechnologies and Hematology \u201cSapienza\u201d University of Rome  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Galimberti","sequence":"additional","affiliation":[{"name":"Division of Hematology Department of Oncology Santa Chiara Hospital  Pisa Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Agostinelli","sequence":"additional","affiliation":[{"name":"Hematopathology Section Department of Experimental, Diagnostic, and Specialty Medicine S. Orsola\u2010Malpighi Hospital Bologna University  Bologna Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P. P.","family":"Piccaluga","sequence":"additional","affiliation":[{"name":"Hematopathology Section Department of Experimental, Diagnostic, and Specialty Medicine S. Orsola\u2010Malpighi Hospital Bologna University  Bologna Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Ladetto","sequence":"additional","affiliation":[{"name":"Division of Hematology Azienda Ospedaliera SS Antonio e Biagio e Cesare Arrigo  Alessandria Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Ferrero","sequence":"additional","affiliation":[{"name":"Department of Molecular Biotechnologies and Health Sciences Division of Hematology University of Torino  Torino Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"Fondazione Italiana Linfomi (FIL) MRD Network","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,12,4]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.2403"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0104566"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2008-11-185132"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2013-06-507319"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2009-06-230250"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2014.219"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-62703-269-8_10"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(87)90083-4"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.13001"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.990200410"},{"key":"e_1_2_8_12_1","first-page":"1","article-title":"Comparison of different DNA extraction methods from peripheral blood cells: advice from the Fondazione Italiana Linfomi Minimal Residual Disease Network","volume":"11","author":"Mannu C","year":"2015","journal-title":"Leuk Lymphoma"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2401246"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exphem.2006.08.008"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199111283252201"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-14-0407"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2400801"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gki065"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1083-8791(00)70006-1"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2404586"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bmt.1701094"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/hon.2095"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2352-3026(16)30185-5"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2016.154211"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2016-07-726307"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2017.29"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/hon.989"}],"container-title":["International Journal of Laboratory Hematology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fijlh.12766","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ijlh.12766","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,23]],"date-time":"2023-09-23T22:51:48Z","timestamp":1695509508000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/ijlh.12766"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,12,4]]},"references-count":26,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2018,4]]}},"alternative-id":["10.1111\/ijlh.12766"],"URL":"https:\/\/doi.org\/10.1111\/ijlh.12766","archive":["Portico"],"relation":{},"ISSN":["1751-5521","1751-553X"],"issn-type":[{"value":"1751-5521","type":"print"},{"value":"1751-553X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,12,4]]}}}