{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T15:59:38Z","timestamp":1787932778094,"version":"build-2784847793"},"reference-count":25,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2018,3,24]],"date-time":"2018-03-24T00:00:00Z","timestamp":1521849600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2018,6]]},"abstract":"Abstract<\/jats:title>\n \n Introduction<\/jats:title>\n \n Hereditary spherocytosis (\n HS<\/jats:styled-content>\n ) is the most common inherited hemolytic anemia with heterogeneous clinico\u2010laboratory manifestations. We evaluated the flow\u2010cytometric tests: eosin\u20105\u2019\u2010maleimide (\n EMA<\/jats:styled-content>\n ) and flow\u2010cytometric osmotic fragility test (\n FOFT<\/jats:styled-content>\n ) and the conventional osmotic fragility tests (\n OFT<\/jats:styled-content>\n ) for the diagnosis of hereditary spherocytosis (\n HS<\/jats:styled-content>\n ).\n <\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n One hundred two suspected\n HS<\/jats:styled-content>\n patients underwent\n EMA<\/jats:styled-content>\n ,\n FOFT<\/jats:styled-content>\n , incubated\n OFT<\/jats:styled-content>\n (\n IOFT<\/jats:styled-content>\n ), and room temperature\n OFT<\/jats:styled-content>\n (\n RT<\/jats:styled-content>\n \u2010\n OFT<\/jats:styled-content>\n ). In addition, 10 cases of immune hemolytic anemia (\n IHA<\/jats:styled-content>\n ) were included, and performance of the above 4 tests was evaluated. For\n EMA<\/jats:styled-content>\n and\n FOFT<\/jats:styled-content>\n , 5 normal controls were assessed together with the patients and cutoffs were calculated using receiver\u2010operator\u2010characteristics curve (\n ROC<\/jats:styled-content>\n ) analysis.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n The best cutoff for %\n EMA<\/jats:styled-content>\n decrease was 12.5%, and for\n FOFT<\/jats:styled-content>\n , %residual red cells (%\n RRC<\/jats:styled-content>\n ) was 25.6%. The sensitivity and specificity of\n RT<\/jats:styled-content>\n \u2010\n OFT<\/jats:styled-content>\n was 62.06% and 86.3%, respectively, while that of\n IOFT<\/jats:styled-content>\n was 79.31% and 87.67%, respectively. Both flow cytometric tests performed better. Sensitivity and specificity of\n EMA<\/jats:styled-content>\n was 86.2% and 93.9% respectively, and that of\n FOFT<\/jats:styled-content>\n was 96.6% and 98.63%, respectively. The combination of the\n FOFT<\/jats:styled-content>\n with\n IOFT<\/jats:styled-content>\n or\n EMA<\/jats:styled-content>\n dye\u2010binding test yields a sensitivity of 100%, but with\n EMA<\/jats:styled-content>\n , it had a higher specificity. Hb\/\n MCHC<\/jats:styled-content>\n was a predictor of the severity of the disease while %\n EMA<\/jats:styled-content>\n decrease and %\n RRC<\/jats:styled-content>\n did not correlate with severity of the disease.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n \n Flow\u2010cytometric osmotic fragility test is the best possible single test followed by\n EMA<\/jats:styled-content>\n for diagnosis of\n HS<\/jats:styled-content>\n . A combination of\n FOFT<\/jats:styled-content>\n and\n EMA<\/jats:styled-content>\n can correctly diagnose 100% patients. These tests are likely to replace conventional\n OFT<\/jats:styled-content>\n s in future.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/ijlh.12794","type":"journal-article","created":{"date-parts":[[2018,3,24]],"date-time":"2018-03-24T05:39:06Z","timestamp":1521869946000},"page":"335-342","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["Flow cytometric osmotic fragility test and eosin\u20105\u2019\u2010maleimide dye\u2010binding tests are better than conventional osmotic fragility tests for the diagnosis of hereditary spherocytosis"],"prefix":"10.1111","volume":"40","author":[{"given":"R. D.","family":"Arora","sequence":"first","affiliation":[{"name":"Department of Hematology Sir Ganga Ram Hospital New Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8282-7842","authenticated-orcid":false,"given":"J.","family":"Dass","sequence":"additional","affiliation":[{"name":"Department of Hematology Sir Ganga Ram Hospital New Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Maydeo","sequence":"additional","affiliation":[{"name":"Department of Hematology Sir Ganga Ram Hospital New Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V.","family":"Arya","sequence":"additional","affiliation":[{"name":"Department of Hematology Sir Ganga Ram Hospital New Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N.","family":"Radhakrishnan","sequence":"additional","affiliation":[{"name":"Department of Pediatric Hematology\u2010Oncology Sir Ganga 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