{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T13:42:53Z","timestamp":1790170973648,"version":"4.1.0"},"reference-count":24,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2018,3,6]],"date-time":"2018-03-06T00:00:00Z","timestamp":1520294400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2018,3,6]],"date-time":"2018-03-06T00:00:00Z","timestamp":1520294400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"},{"start":{"date-parts":[[2018,3,6]],"date-time":"2018-03-06T00:00:00Z","timestamp":1520294400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000152","name":"Canadian Hemophilia Society","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000152","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000152","name":"Canadian Hemophilia Society","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000152","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2018,8]]},"abstract":"Abstract<\/jats:title>Introduction<\/jats:title>Dense granule (DG<\/jats:styled-content>) deficiency (DGD<\/jats:styled-content>) is a feature of some platelet function disorders (PFD<\/jats:styled-content>) with a prevalence similar to von Willebrand disease. Most laboratories assess for DGD<\/jats:styled-content> using whole mount platelet preparations and electron microscopy (EM<\/jats:styled-content>). We evaluated our experiences with this test and associations between DGD<\/jats:styled-content> and bleeding.<\/jats:p><\/jats:sec>Methods<\/jats:title>Dense granule EM records for 2006\u20102017 were examined for patients and simultaneously tested controls, and for an overlapping PFD<\/jats:styled-content> study cohort to evaluate findings and their relationship to bleeding.<\/jats:p><\/jats:sec>Results<\/jats:title>More patient than control samples had reduced DG<\/jats:styled-content> counts (6.5% vs 0.3%, P<\/jats:italic>\u00a0<\u00a0.01). DG<\/jats:styled-content> counts showed no relationship to age or mean platelet volume and had acceptable within\u2010subject variability that was higher for DGD<\/jats:styled-content> than control participants (28% vs 12%). Repeat tests confirmed DGD<\/jats:styled-content> in all persons with initial DG<\/jats:styled-content> counts <4.0\/platelet, but not in those with less severe reductions (4.0\u20104.8 DG<\/jats:styled-content>\/platelet) or normal DG<\/jats:styled-content> counts (\u22654.9 DG<\/jats:styled-content>\/platelet). Aggregometry and adenosine triphosphate release tests, respectively, had only ~52% and 70% sensitivity for DGD<\/jats:styled-content>. Confirmed DGD<\/jats:styled-content> by EM<\/jats:styled-content> was associated with higher bleeding scores and a bleeding disorder.<\/jats:p><\/jats:sec>Conclusion<\/jats:title>Whole mount EM<\/jats:styled-content> is useful for the evaluation of suspected PFD<\/jats:styled-content> due to DGD<\/jats:styled-content> and detects abnormalities associated with bleeding.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijlh.12801","type":"journal-article","created":{"date-parts":[[2018,3,6]],"date-time":"2018-03-06T07:06:40Z","timestamp":1520320000000},"page":"400-407","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":56,"title":["Electron microscopy examination of platelet whole mount preparations to quantitate platelet dense granule numbers: Implications for diagnosing suspected platelet function disorders due to dense granule deficiency"],"prefix":"10.1111","volume":"40","author":[{"given":"J. G.","family":"Brunet","sequence":"first","affiliation":[{"name":"Department of Pathology and Molecular Medicine McMaster University Hamilton ON Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. K.","family":"Iyer","sequence":"additional","affiliation":[{"name":"Department of Pathology and Molecular Medicine McMaster University Hamilton ON Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. S.","family":"Badin","sequence":"additional","affiliation":[{"name":"Department of Pathology and Molecular Medicine McMaster University Hamilton ON Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Graf","sequence":"additional","affiliation":[{"name":"Centre for Laboratory Medicine and Hemophilia and Hemostasis Centre St. Gallen Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K. A.","family":"Moffat","sequence":"additional","affiliation":[{"name":"Department of Medicine McMaster University Hamilton ON Canada"},{"name":"Hamilton Regional Laboratory Medicine Program Hamilton ON Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Timleck","sequence":"additional","affiliation":[{"name":"Department of Pathology and Molecular Medicine McMaster University Hamilton ON Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Spitzer","sequence":"additional","affiliation":[{"name":"Department of Pathology and Molecular Medicine McMaster University Hamilton ON Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2843-0817","authenticated-orcid":false,"given":"C. P. 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