{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T18:41:44Z","timestamp":1760985704947,"version":"3.37.3"},"reference-count":19,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2018,5,13]],"date-time":"2018-05-13T00:00:00Z","timestamp":1526169600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Queen Elizabeth Hospital Birmingham Charity"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2018,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>Lutheran\/<jats:styled-content style=\"fixed-case\">BCAM<\/jats:styled-content> protein (Lu) on the surface of erythrocytes is key for their adhesion to the endothelium, and erythrocytes from individuals with <jats:styled-content style=\"fixed-case\">JAK<\/jats:styled-content>2V617F\u2010mutated myeloproliferative neoplasms (<jats:styled-content style=\"fixed-case\">MPN<\/jats:styled-content>) have increased endothelial adhesion. Splanchnic vein thrombosis (<jats:styled-content style=\"fixed-case\">SVT<\/jats:styled-content>) is a devastating thrombotic complication of <jats:styled-content style=\"fixed-case\">MPN<\/jats:styled-content>, and frequently, the only diagnostic feature is the <jats:styled-content style=\"fixed-case\">JAK<\/jats:styled-content>2V617F mutation. We sought to examine whether erythrocytes from patients with JAK2V617F mutated <jats:styled-content style=\"fixed-case\">SVT<\/jats:styled-content> (MPN\u2010SVT) exhibited increased Lu expression, thereby supporting a mechanistic contribution to the development of thrombosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We report the validation of a novel flow cytometry assay for Lu expression on erythrocytes. We examined the expression of Lu on erythrocytes from a cohort of <jats:styled-content style=\"fixed-case\">MPN<\/jats:styled-content> patients with and without <jats:styled-content style=\"fixed-case\">SVT<\/jats:styled-content>, and healthy controls. Samples were obtained from 20 normal individuals, 22 with <jats:styled-content style=\"fixed-case\">MPN<\/jats:styled-content> (both <jats:styled-content style=\"fixed-case\">JAK<\/jats:styled-content>2V617F\u2010mutated and wild\u2010type) and 8 with <jats:styled-content style=\"fixed-case\">JAK<\/jats:styled-content>2V617F\u2010mutated MPN\u2010<jats:styled-content style=\"fixed-case\">SVT<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Lu expression by erythrocytes from patients with <jats:styled-content style=\"fixed-case\">MPN<\/jats:styled-content> and MPN\u2010<jats:styled-content style=\"fixed-case\">SVT<\/jats:styled-content> is significantly increased compared to erythrocytes from healthy individuals (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.05), but there was no significant difference between patients with MPN\u2010<jats:styled-content style=\"fixed-case\">SVT<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">MPN<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Patients with <jats:styled-content style=\"fixed-case\">MPN<\/jats:styled-content> have increased expression of the red cell Lu\/<jats:styled-content style=\"fixed-case\">BCAM<\/jats:styled-content> adhesion molecule. Further work is required to determine the role of the increased Lu\/<jats:styled-content style=\"fixed-case\">BCAM<\/jats:styled-content> adhesion to the endothelium in the development of thrombosis in <jats:styled-content style=\"fixed-case\">MPN<\/jats:styled-content> of all genotypes.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijlh.12838","type":"journal-article","created":{"date-parts":[[2018,5,14]],"date-time":"2018-05-14T05:50:20Z","timestamp":1526277020000},"page":"473-477","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Erythrocytes from patients with myeloproliferative neoplasms and splanchnic venous thrombosis show greater expression of Lu\/<scp>BCAM<\/scp>"],"prefix":"10.1111","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3748-3117","authenticated-orcid":false,"given":"I.","family":"Novitzky\u2010Basso","sequence":"first","affiliation":[{"name":"Scottish Blood and Marrow Transplant Unit Queen Elizabeth University Hospital  Glasgow UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F.","family":"Spring","sequence":"additional","affiliation":[{"name":"NHS Blood and Transplant  Filton, Bristol UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.","family":"Anstee","sequence":"additional","affiliation":[{"name":"NHS Blood and Transplant  Filton, Bristol UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.","family":"Tripathi","sequence":"additional","affiliation":[{"name":"Liver Unit, Queen Elizabeth Hospital Birmingham NHS Trust  Birmingham UK"},{"name":"Centre for Rare Disease University of Birmingham  Birmingham UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F.","family":"Chen","sequence":"additional","affiliation":[{"name":"Centre for Clinical Haematology Queen Elizabeth Hospital Birmingham NHS Trust  Birmingham UK"},{"name":"Centre for Rare Disease University of Birmingham  Birmingham 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