{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T13:46:29Z","timestamp":1792071989095,"version":"4.4.0"},"reference-count":29,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2018,5,27]],"date-time":"2018-05-27T00:00:00Z","timestamp":1527379200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"SydPath, St Vincent\u2019s Hospital Sydney"},{"name":"St. Vincent\u2019s Clinical School, Faculty of Medicine, UNSW Sydney"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2018,10]]},"abstract":"Abstract<\/jats:title>Introduction<\/jats:title>Immunophenotyping by flow cytometry is routinely employed in distinguishing between chronic lymphocytic leukemia (CLL<\/jats:styled-content>) and mantle cell lymphoma (MCL<\/jats:styled-content>). Inclusion of CD<\/jats:styled-content>200 has been reported to contribute to more reliable differentiation between CLL<\/jats:styled-content> and MCL<\/jats:styled-content>. We investigated the value of CD<\/jats:styled-content>200 in assessment of atypical CLL<\/jats:styled-content> cases.<\/jats:p><\/jats:sec>Methods<\/jats:title>CD<\/jats:styled-content>200 expression on mature B cell neoplasms was studied by eight\u2010color flow cytometry in combination with a conventional panel of flow cytometry markers. The study included 70 control samples, 63 samples with CLL<\/jats:styled-content> or atypical CLL<\/jats:styled-content> phenotype, 6 MCL<\/jats:styled-content> samples, and 40 samples of other mature B cell neoplasms.<\/jats:p><\/jats:sec>Results<\/jats:title>All CLL<\/jats:styled-content> samples were positive for CD<\/jats:styled-content>200, whereas MCL<\/jats:styled-content> samples were dim or negative for CD<\/jats:styled-content>200. Of the CLL<\/jats:styled-content> samples, 7 were atypical by conventional flow cytometry, with Matutes scores \u22643. These cases were tested for evidence of a t(11;14) translocation, characteristic of MCL<\/jats:styled-content>, and all were negative, consistent with their classification as atypical CLL<\/jats:styled-content>. All these atypical CLL<\/jats:styled-content> samples were strongly positive for CD<\/jats:styled-content>200.<\/jats:p><\/jats:sec>Conclusion<\/jats:title>CD<\/jats:styled-content>200 proved to be a useful marker for differentiation between CLL<\/jats:styled-content> and MCL<\/jats:styled-content> by flow cytometry. In particular, CD<\/jats:styled-content>200 was useful in distinguishing CLL<\/jats:styled-content> samples with atypical immunophenotypes from MCL<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijlh.12857","type":"journal-article","created":{"date-parts":[[2018,5,28]],"date-time":"2018-05-28T04:45:38Z","timestamp":1527482738000},"page":"533-539","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["CD<\/scp>200 is a useful diagnostic marker for identifying atypical chronic lymphocytic leukemia by flow cytometry"],"prefix":"10.1111","volume":"40","author":[{"given":"Y. S.","family":"Ting","sequence":"first","affiliation":[{"name":"St Vincent\u2019s Pathology (SydPath) St Vincent\u2019s Hospital Sydney Darlinghurst NSW Australia"},{"name":"St Vincent\u2019s Clinical School UNSW Sydney Sydney NSW Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S. A. B. 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