{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T14:12:56Z","timestamp":1740147176559,"version":"3.37.3"},"reference-count":28,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2018,6,20]],"date-time":"2018-06-20T00:00:00Z","timestamp":1529452800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004901","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Minas Gerais","doi-asserted-by":"publisher","award":["APQ\u201003482\u201013"],"award-info":[{"award-number":["APQ\u201003482\u201013"]}],"id":[{"id":"10.13039\/501100004901","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2018,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>Diagnosis of von Willebrand disease (<jats:styled-content style=\"fixed-case\">VWD<\/jats:styled-content>) is difficult due to the heterogenic phenotype of patients and to the complex tests that are required for an adequate investigation. The collagen binding assay (<jats:styled-content style=\"fixed-case\">VWF<\/jats:styled-content>:<jats:styled-content style=\"fixed-case\">CB<\/jats:styled-content>) reveals the adhesion capacity of von Willebrand factor (<jats:styled-content style=\"fixed-case\">VWF<\/jats:styled-content>) to collagen and can be useful to reduce the misleading diagnosis of<jats:styled-content style=\"fixed-case\">VWD<\/jats:styled-content>. This study aimed the standardization of 2 nonautomated<jats:styled-content style=\"fixed-case\">VWF<\/jats:styled-content>:<jats:styled-content style=\"fixed-case\">CB<\/jats:styled-content>assays based on<jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content>and flow cytometry.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Plasma samples from 87 patients previously diagnosed with<jats:styled-content style=\"fixed-case\">VWD<\/jats:styled-content>and 22 healthy controls were analyzed. Measurement of the<jats:styled-content style=\"fixed-case\">VWF<\/jats:styled-content>\u2010collagen binding activity was performed using a commercial assay and the 2 tests proposed.<jats:styled-content style=\"fixed-case\">VWF<\/jats:styled-content>:<jats:styled-content style=\"fixed-case\">CB<\/jats:styled-content>\/<jats:styled-content style=\"fixed-case\">VWF<\/jats:styled-content>:Ag ratio was calculated for samples and the differentiation between types 1, 2A, and 2M was analyzed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content>and flow cytometry tests presented strong correlation with the gold standard test (<jats:italic>r<\/jats:italic><jats:sup>2<\/jats:sup>\u00a0=\u00a0.8976 and<jats:italic>r<\/jats:italic><jats:sup>2<\/jats:sup>\u00a0=\u00a0.8143, respectively). Tests based on<jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content>and flow cytometry presented a bias of +7.2% and \u22123.6%, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The<jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content>test demonstrated better performance to detect<jats:styled-content style=\"fixed-case\">VWF<\/jats:styled-content>\u2010collagen binding activity in healthy individuals and<jats:styled-content style=\"fixed-case\">VWD<\/jats:styled-content>patients. This test could differentiate 2A and 2M subtypes using a feasible protocol that can be easily implemented.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijlh.12874","type":"journal-article","created":{"date-parts":[[2018,6,20]],"date-time":"2018-06-20T14:55:20Z","timestamp":1529506520000},"page":"597-603","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Standardization and comparison of nonautomated assays to measure the collagen binding activity of von Willebrand factor"],"prefix":"10.1111","volume":"40","author":[{"given":"L. M. M.","family":"Oliveira","sequence":"first","affiliation":[{"name":"Funda\u00e7\u00e3o Hemominas Belo Horizonte Minas Gerais Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. V. 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