{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T11:18:27Z","timestamp":1788779907151,"version":"build-2803163510"},"reference-count":16,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2018,8,17]],"date-time":"2018-08-17T00:00:00Z","timestamp":1534464000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2018,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>Hb H inclusion test (HbH\u2010i) commonly used for \u03b1\u2010thalassaemia screening is not standardised and is labour\u2010intensive. This study evaluated a strip test based on immunochromatographic detection of Hb Bart's (<jats:styled-content style=\"fixed-case\">ICT<\/jats:styled-content>) for use as a routine screening test for \u03b1\u2010thalassaemia screening in the clinical laboratory setting.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The performance characteristics of the<jats:styled-content style=\"fixed-case\">ICT<\/jats:styled-content>was determined by comparing the results of<jats:styled-content style=\"fixed-case\">ICT<\/jats:styled-content>and HbH\u2010i on 67 patients, and the \u03b1\u2010globin genotype on 47 of these patients who also had the molecular analysis. Specimen stability was tested on 16 specimens with the<jats:styled-content style=\"fixed-case\">ICT<\/jats:styled-content>repeated after 7\u00a0days of storage. The age of babies from which the<jats:styled-content style=\"fixed-case\">ICT<\/jats:styled-content>result becomes valid was determined on 49 samples with patient age ranged from 4\u00a0weeks to 12\u00a0months.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The<jats:styled-content style=\"fixed-case\">ICT<\/jats:styled-content>had higher overall sensitivity of 76% compared to 24% for HbH\u2010i in detecting carriers of \u03b1\u2010thalassaemia mutations, and this is seen in all \u03b1\u2010thalassaemia genotypes. The test could be carried out on specimens stored at 4\u00b0C for 7\u00a0days and gave valid results with no false positive from the age of 6\u00a0months onwards. It required no special technical expertise or equipment and gave the result in less than 5\u00a0minutes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The<jats:styled-content style=\"fixed-case\">ICT<\/jats:styled-content>is simple to perform, with higher sensitivity than HbH\u2010i, and gives the result in a short time and at a lower cost. This can be used by clinical laboratories to replace HbH\u2010i for \u03b1\u2010thalassaemia detection.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijlh.12905","type":"journal-article","created":{"date-parts":[[2018,8,17]],"date-time":"2018-08-17T20:09:53Z","timestamp":1534536593000},"page":"691-696","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Evaluation of an immunochromatographic strip test for alpha\u2010thalassaemia screening"],"prefix":"10.1111","volume":"40","author":[{"given":"Carolyn","family":"Bunkall","sequence":"first","affiliation":[{"name":"LabPlus Haematology Department Auckland City Hospital Auckland New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nikhil","family":"Ghallyan","sequence":"additional","affiliation":[{"name":"LabPlus Diagnostic Genetics Department Auckland City Hospital Auckland New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine","family":"Elliott","sequence":"additional","affiliation":[{"name":"LabPlus Haematology Department Auckland City Hospital Auckland New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neil","family":"Van de Water","sequence":"additional","affiliation":[{"name":"LabPlus Diagnostic Genetics Department Auckland City Hospital Auckland New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4316-6177","authenticated-orcid":false,"given":"George","family":"Chan","sequence":"additional","affiliation":[{"name":"LabPlus Haematology Department Auckland City Hospital Auckland New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,8,17]]},"reference":[{"key":"e_1_2_8_2_1","unstructured":"Modell's Haemoglobinopathologist's Almanac 2008 [Internet]. UCL Centre for Health Informatics and Multiprofessional Education.London.http:\/\/www.modell-almanac.net\/. Accessed 19 January 2018."},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2010-01-251348"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra1404415"},{"key":"e_1_2_8_5_1","doi-asserted-by":"crossref","first-page":"2213","DOI":"10.1182\/blood.V91.7.2213","article-title":"Hydrops fetalis caused by \u03b1\u2010thalassemia: an emerging health care problem","volume":"40","author":"Chui DHK","year":"1998","journal-title":"Blood"},{"key":"e_1_2_8_6_1","first-page":"493","article-title":"The inherited disorders of haemoglobin: an increasingly neglected global health burden","volume":"40","author":"Weatherall D","year":"2011","journal-title":"Indian J Med Res"},{"key":"e_1_2_8_7_1","first-page":"7","article-title":"The clinical significance of occasional red\u2010cells with Haemoglobin H inclusions","volume":"40","author":"Kwan CS","year":"1977","journal-title":"J Hong Kong Medi Technol Assoc"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1159\/000206353"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12185-009-0331-4"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trsl.2014.10.013"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V98.1.250"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1993.tb04639.x"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2257.1985.tb00043.x"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.3109\/00313027909059042"},{"key":"e_1_2_8_15_1","doi-asserted-by":"crossref","first-page":"462","DOI":"10.18295\/2075-0528.1315","article-title":"Mean haemoglobin and red cell indices in cord blood from Omani neonates","volume":"40","author":"Alkindi S","year":"2011","journal-title":"Sultan Qaboos Univ Med J"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cca.2013.09.041"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1177\/0969141314538611"}],"container-title":["International Journal of Laboratory Hematology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fijlh.12905","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ijlh.12905","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,7,6]],"date-time":"2025-07-06T11:46:20Z","timestamp":1751802380000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/ijlh.12905"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,8,17]]},"references-count":16,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2018,12]]}},"alternative-id":["10.1111\/ijlh.12905"],"URL":"https:\/\/doi.org\/10.1111\/ijlh.12905","archive":["Portico"],"relation":{},"ISSN":["1751-5521","1751-553X"],"issn-type":[{"value":"1751-5521","type":"print"},{"value":"1751-553X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,8,17]]},"assertion":[{"value":"2018-02-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-07-06","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-08-17","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}