{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T03:13:14Z","timestamp":1773025994396,"version":"3.50.1"},"reference-count":18,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2018,9,5]],"date-time":"2018-09-05T00:00:00Z","timestamp":1536105600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2018,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>Development of additional parameters for complete blood count has emerged in recent hematology analyzers, leading to many publications. However, few studies have been conducted on advanced <jats:styled-content style=\"fixed-case\">RBC<\/jats:styled-content> parameters and hemolytic anemias. We investigated the interest of Sysmex unique parameters, MicroR and HypoHe, as well as the immature fraction of reticulocytes (<jats:styled-content style=\"fixed-case\">IRF<\/jats:styled-content>) in combination with complete blood and reticulocyte count, for screening hereditary spherocytosis (HS) and pyruvate kinase deficiency.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We analyzed 182 samples using Sysmex <jats:styled-content style=\"fixed-case\">XE<\/jats:styled-content>\u20105000 analyzers from a cohort of red cell disorder patients from the Rouen University Hospital. These included 47 HS, 17 pyruvate kinase deficiencies, sickle cell diseases and trait, \u03b2\u2010thalassemia minor, iron deficiencies, and 489 samples from a routine group.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Combining five parameters (hemoglobin level, reticulocyte count, <jats:styled-content style=\"fixed-case\">IRF<\/jats:styled-content>, MicroR, and %HypoHe), we developed a specific screening tool for HS allowing a sensitivity of 100% and a specificity of 92.1% and a specific screening tool for pyruvate kinase deficiencies allowing a sensitivity of 100% and a specificity of 96.5%. These parameters were also found accurate in infants and in HS without anemia.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>We propose a costless, easy\u2010to\u2010use, and efficient approach to detect HS and pyruvate kinase deficiencies using Sysmex analyzers. These screening tools may help diagnosis of these disorders, help prevent complications, and result in a better management of these patients.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijlh.12906","type":"journal-article","created":{"date-parts":[[2018,9,5]],"date-time":"2018-09-05T14:42:35Z","timestamp":1536158555000},"page":"697-703","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Screening of hereditary spherocytosis and pyruvate kinase deficiency by automated blood count using erythrocytic and reticulocytic parameters"],"prefix":"10.1111","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9357-4542","authenticated-orcid":false,"given":"Victor","family":"Bob\u00e9e","sequence":"first","affiliation":[{"name":"Department of Biological Hematology Rouen University Hospital  Rouen France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sylvie","family":"Daliphard","sequence":"additional","affiliation":[{"name":"Department of Biological Hematology Rouen University Hospital  Rouen France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aur\u00e9lien","family":"Schrapp","sequence":"additional","affiliation":[{"name":"Department of Biological Hematology Rouen University Hospital  Rouen France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Agn\u00e8s","family":"Lahary","sequence":"additional","affiliation":[{"name":"Department of Biological Hematology Rouen University Hospital  Rouen France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,9,5]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2004.04987.x"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3148.2006.00654.x"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/pbc.24484"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcla.20123"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/ijlh.12335"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.blre.2006.03.005"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(08)61588-3"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21511"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1542\/peds.2009-0864"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-553X.2009.01216.x"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2143(96)90027-X"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00277-010-1138-3"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00277-011-1243-y"},{"key":"e_1_2_7_15_1","doi-asserted-by":"crossref","first-page":"3585","DOI":"10.1182\/blood.V95.11.3585","article-title":"Estimating the prevalence of pyruvate kinase deficiency from the gene frequency in the general white population","volume":"40","author":"Beutler E","year":"2000","journal-title":"Blood"},{"key":"e_1_2_7_16_1","doi-asserted-by":"crossref","first-page":"4005","DOI":"10.1182\/blood.V96.12.4005","article-title":"Prevalence of pyruvate kinase deficiency in northern European population in the north of England. 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