{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T06:07:01Z","timestamp":1760854021257,"version":"3.37.3"},"reference-count":18,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2018,8,16]],"date-time":"2018-08-16T00:00:00Z","timestamp":1534377600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2018,12]]},"abstract":"Abstract<\/jats:title>Introduction<\/jats:title>The discrimination of leukemia lymphoblasts (LB<\/jats:styled-content>) in diagnosis and follow\u2010up of B\u2010cell progenitor acute lymphoblastic leukemia (BCP<\/jats:styled-content>\u2010ALL<\/jats:styled-content>) by multiparameter flow cytometry (MFC<\/jats:styled-content>) may be difficult due to the presence of hematogones (HG<\/jats:styled-content>). The aim of this study was to compare lymphoblasts of BCP<\/jats:styled-content>\u2010ALL<\/jats:styled-content> and HG<\/jats:styled-content> for the expression of the most discriminating antigens.<\/jats:p><\/jats:sec>Methods<\/jats:title>A total of 82 bone marrow samples (39 BCP<\/jats:styled-content>\u2010ALL<\/jats:styled-content> and 43 patients with HG<\/jats:styled-content>) were analyzed using MFC<\/jats:styled-content>. Mean fluorescence intensity (MFI<\/jats:styled-content>) was measured for ten markers commonly used in hematology laboratories: CD<\/jats:styled-content>45, CD<\/jats:styled-content>19, CD<\/jats:styled-content>10, CD<\/jats:styled-content>34, CD<\/jats:styled-content>38, CD<\/jats:styled-content>20, CD<\/jats:styled-content>22, CD<\/jats:styled-content>58, CD<\/jats:styled-content>81, and CD<\/jats:styled-content>123. Statistical comparison of the MFI<\/jats:styled-content> between LB<\/jats:styled-content> and HG<\/jats:styled-content> was performed. The presence on LB<\/jats:styled-content> of aberrant expression of myeloid and\/or T\u2010cell markers was also investigated.<\/jats:p><\/jats:sec>Results<\/jats:title>Qualitative pattern expression of antigens showed overexpression on LB<\/jats:styled-content> of CD<\/jats:styled-content>58, CD<\/jats:styled-content>22, CD<\/jats:styled-content>34, CD<\/jats:styled-content>10 and underexpression of CD<\/jats:styled-content>81, CD<\/jats:styled-content>45, CD<\/jats:styled-content>38 when compared to HG<\/jats:styled-content>. Expression of CD<\/jats:styled-content>123 was positive in 34% of BCP<\/jats:styled-content>\u2010ALL LB<\/jats:styled-content> and always absent on HG<\/jats:styled-content>. Aberrant antigen expression (myeloid and\/or T\u2010cell marker) including CD<\/jats:styled-content>123 was observed in 58% of BCP<\/jats:styled-content>\u2010ALL<\/jats:styled-content> patients. The use of a MFI<\/jats:styled-content> antigen ratio of the most discriminating markers (CD<\/jats:styled-content>81\/CD<\/jats:styled-content>58) (analysis of variance, P<\/jats:italic>\u00a0<\u00a00.005) increased the distinction of LB<\/jats:styled-content> versus HG<\/jats:styled-content> with a high specificity and sensitivity as demonstrated by the use of ROC<\/jats:styled-content> curve analysis (AUC<\/jats:styled-content> of CD<\/jats:styled-content>81\/CD<\/jats:styled-content>58: 0.995).<\/jats:p><\/jats:sec>Conclusion<\/jats:title>We demonstrate in this study that routine use of the MFI<\/jats:styled-content> antigen ratio (CD<\/jats:styled-content>81\/CD<\/jats:styled-content>58) in addition to the MFC<\/jats:styled-content> evaluation using WHO<\/jats:styled-content> classical criteria appears to be an efficient approach to discriminate LB<\/jats:styled-content> from HG<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijlh.12912","type":"journal-article","created":{"date-parts":[[2018,8,16]],"date-time":"2018-08-16T14:10:42Z","timestamp":1534428642000},"page":"734-739","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Easy discrimination of hematogones from lymphoblasts in B\u2010cell progenitor acute lymphoblastic leukemia patients using CD<\/scp>81\/CD<\/scp>58 expression ratio"],"prefix":"10.1111","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9755-5069","authenticated-orcid":false,"given":"Carole","family":"Nagant","sequence":"first","affiliation":[{"name":"Laboratoire d'H\u00e9matologie R\u00e9seau des Laboratoires Hospitaliers Universitaires de Bruxelles (LHUB\u2010ULB) Bruxelles Belgique"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniele","family":"Casula","sequence":"additional","affiliation":[{"name":"Laboratoire d'H\u00e9matologie R\u00e9seau des Laboratoires Hospitaliers Universitaires de Bruxelles (LHUB\u2010ULB) Bruxelles Belgique"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anne","family":"Janssens","sequence":"additional","affiliation":[{"name":"Laboratoire d'H\u00e9matologie R\u00e9seau des Laboratoires Hospitaliers Universitaires de Bruxelles (LHUB\u2010ULB) Bruxelles Belgique"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vo Thanh Phuong","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Laboratoire d'H\u00e9matologie R\u00e9seau des Laboratoires Hospitaliers Universitaires de Bruxelles (LHUB\u2010ULB) Bruxelles Belgique"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brigitte","family":"Cantinieaux","sequence":"additional","affiliation":[{"name":"Laboratoire d'H\u00e9matologie R\u00e9seau des Laboratoires Hospitaliers Universitaires de Bruxelles (LHUB\u2010ULB) Bruxelles 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