{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T16:04:07Z","timestamp":1781798647119,"version":"3.54.5"},"reference-count":22,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2018,9,24]],"date-time":"2018-09-24T00:00:00Z","timestamp":1537747200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2019,2]]},"abstract":"Abstract<\/jats:title>Introduction<\/jats:title>A prolonged activated partial thromboplastin time (APTT<\/jats:styled-content>) may be indicative of a specific or multiple factor deficiency, therapeutic anticoagulation, presence of a nonspecific factor inhibitor, or lupus anticoagulant (LA<\/jats:styled-content>). Recently, pairing of the LA<\/jats:styled-content>\u2010sensitive APTT<\/jats:styled-content> and standard APTT<\/jats:styled-content> reagents, Cephen LS<\/jats:styled-content> and Cephen, respectively, has been shown to be effective in LA<\/jats:styled-content> detection. The present study aimed to evaluate the usefulness of this reagent pair for discriminating between causes of APTT<\/jats:styled-content> elevation and the detection of LA<\/jats:styled-content> in conjunction with dilute Russell's viper venom time (dRVVT<\/jats:styled-content>).<\/jats:p><\/jats:sec>Methods<\/jats:title>Plasma samples from 50 normal and 105 non\u2010anticoagulated LA<\/jats:styled-content>\u2010positive patients in routine dRVVT<\/jats:styled-content> and\/or dilute APTT<\/jats:styled-content> (dAPTT<\/jats:styled-content>) via the percent correction formula were employed. Cephen LS<\/jats:styled-content>\/Cephen and dRVVT<\/jats:styled-content> reagents LA<\/jats:styled-content>1\/LA<\/jats:styled-content>2 were used to screen\/confirm, respectively. Thirty\u2010four symptomatic LA<\/jats:styled-content>\u2010negative, 25 warfarinised non\u2010antiphospholipid syndrome, 6 coagulation inhibitors, 17 samples with hereditary elevated APTT<\/jats:styled-content>, and 24 FVIII<\/jats:styled-content>\/IX<\/jats:styled-content>\/XI<\/jats:styled-content>\/XII<\/jats:styled-content> and 17 FII<\/jats:styled-content>\/V\/X artificial single deficiency plasmas were used.<\/jats:p><\/jats:sec>Results<\/jats:title>Thirty\u2010three samples out of 105 (31%) were LA<\/jats:styled-content>\u2010positive in Cephen LS<\/jats:styled-content>\/Cephen. The total percent positivity in Cephen LS<\/jats:styled-content>\/Cephen and LA<\/jats:styled-content>1\/LA<\/jats:styled-content>2 pairs was 89.1% against samples with the routine dRVVT<\/jats:styled-content>\/dAPTT<\/jats:styled-content> double positive. The percent corrections of Cephen LS<\/jats:styled-content>\/Cephen in the routine dAPTT<\/jats:styled-content>\/dRVVT<\/jats:styled-content> positive group were significantly higher than those in all other groups.<\/jats:p><\/jats:sec>Conclusions<\/jats:title>The percent correction of the APTT<\/jats:styled-content> reagent pair showed higher values in LA<\/jats:styled-content>\u2010positive samples. The combination will be useful with respect to differentiating LA<\/jats:styled-content> from other abnormal samples and is effective in LA<\/jats:styled-content> detection when paired with dRVVT<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijlh.12921","type":"journal-article","created":{"date-parts":[[2018,9,24]],"date-time":"2018-09-24T17:20:16Z","timestamp":1537809616000},"page":"60-68","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Paired APTT<\/scp>s of low and high lupus anticoagulant sensitivity permit distinction from other abnormalities and achieve good lupus anticoagulant detection rates in conjunction with dRVVT<\/scp>"],"prefix":"10.1111","volume":"41","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6650-0567","authenticated-orcid":false,"given":"Osamu","family":"Kumano","sequence":"first","affiliation":[{"name":"Protein Technology Sysmex Corporation Kobe Hyogo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean","family":"Amiral","sequence":"additional","affiliation":[{"name":"Hyphen BioMed Neuville sur Oise France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claire","family":"Dunois","sequence":"additional","affiliation":[{"name":"Hyphen BioMed Neuville sur Oise France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie","family":"Peyrafitte","sequence":"additional","affiliation":[{"name":"Hyphen BioMed Neuville sur Oise France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gary W.","family":"Moore","sequence":"additional","affiliation":[{"name":"Department of Haemostasis and Thrombosis Viapath Analytics, Guy's & St Thomas\u2019 Hospitals London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,9,24]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/ijlh.12025"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-553X.2010.01266.x"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0038-1649988"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2012.09037.x"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/ijlh.12210"},{"key":"e_1_2_8_7_1","first-page":"120","article-title":"The sensitivity of different coagulation reagents to the presence of lupus anticoagulants","volume":"111","author":"Brandt JT","year":"1987","journal-title":"Arch Pathol Lab Med"},{"key":"e_1_2_8_8_1","first-page":"98","article-title":"Sensitivity of sixteen APTT reagents for the presence of lupus anticoagulants","volume":"25","author":"Denis\u2010Magdelaine A","year":"1995","journal-title":"Hamostasis"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1538-7836.2012.04906.x"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/ejh.12634"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1097\/00001721-199704000-00001"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1538-7836.2009.03555.x"},{"key":"e_1_2_8_13_1","volume-title":"Laboratory Testing for the Lupus Anticoagulant; 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