{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,5]],"date-time":"2026-03-05T14:21:01Z","timestamp":1772720461058,"version":"3.50.1"},"reference-count":12,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2019,1,20]],"date-time":"2019-01-20T00:00:00Z","timestamp":1547942400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2019,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>The accurate determination of Hb A<jats:sub>2<\/jats:sub> is a key marker when screening for a \u03b2\u2010thalassaemia carrier. Data from external quality assessment (<jats:styled-content style=\"fixed-case\">EQA<\/jats:styled-content>) exercises have shown a lack of alignment of Hb A<jats:sub>2<\/jats:sub> quantitation both within and between methods. The only reference material available for Hb A<jats:sub>2<\/jats:sub> quantitative assay at the time of writing is the World Health Organization International Reference Reagent (89\/666; <jats:styled-content style=\"fixed-case\">WHO IRR<\/jats:styled-content>) prepared in the 1980s and not validated for all current methodologies.<\/jats:p><\/jats:sec><jats:sec><jats:title>Method<\/jats:title><jats:p>The <jats:styled-content style=\"fixed-case\">WHO IRR<\/jats:styled-content> was analysed for Hb A<jats:sub>2<\/jats:sub> concentration by 52 laboratories using a representative range of high\u2010performance liquid chromatography and capillary electrophoresis analysers. The results of the analysis were compared to those of a whole blood <jats:styled-content style=\"fixed-case\">EQA<\/jats:styled-content> specimen of similar Hb A<jats:sub>2<\/jats:sub> concentration, distributed in the same week.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The mean Hb A<jats:sub>2<\/jats:sub> value obtained for the <jats:styled-content style=\"fixed-case\">WHO IRR<\/jats:styled-content> was 5.17%, compared to the assigned value of 5.3%. The range of results returned was wide (4.0%\u20106.2%), with differences in the results observed by between and within analyser groups. A similar range of results was seen with the whole blood sample, although the bias observed between analyser types was different from that seen with the <jats:styled-content style=\"fixed-case\">WHO IRR<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The results may indicate a lack of commutability of the <jats:styled-content style=\"fixed-case\">WHO IRR<\/jats:styled-content> material, resulting from deterioration, matrix effects or changes in reagent formulation or calibration parameters. Further examination of the suitability of the <jats:styled-content style=\"fixed-case\">WHO IRR<\/jats:styled-content> (89\/666) for continued use is required.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijlh.12966","type":"journal-article","created":{"date-parts":[[2019,1,21]],"date-time":"2019-01-21T06:48:26Z","timestamp":1548053306000},"page":"262-270","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Evaluation of the suitability of the World Health Organization International Reference Reagent for Hb A<sub>2<\/sub> quantitation (89\/666) for continued use"],"prefix":"10.1111","volume":"41","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2926-7441","authenticated-orcid":false,"given":"Barbara","family":"De la Salle","sequence":"first","affiliation":[{"name":"UK NEQAS Haematology Watford UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adrian D.","family":"Stephens","sequence":"additional","affiliation":[{"name":"UK NEQAS Haematology Watford UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barbara J.","family":"Wild","sequence":"additional","affiliation":[{"name":"UK NEQAS Haematology Watford UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cornelis L.","family":"Harteveld","sequence":"additional","affiliation":[{"name":"University Medical Centre Leiden The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Keith","family":"Hyde","sequence":"additional","affiliation":[{"name":"Manchester Metropolitan University Manchester UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,1,20]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-553X.2011.01368.x"},{"key":"e_1_2_7_3_1","unstructured":"BatterbeeH.Evaluation of UK NEQAS (H) Hb A2 and related performance data. 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