{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T19:46:37Z","timestamp":1774467997334,"version":"3.50.1"},"reference-count":13,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2019,3,4]],"date-time":"2019-03-04T00:00:00Z","timestamp":1551657600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2019,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>The standard screening method for alpha thalassaemia is the examination of HbH preparation. It is labour intensive and poorly standardized. The development of a rapid strip immunochromatographic test (ICT) for haemoglobin Barts offers a fast, user friendly and cost\u2010effective alternative screening tool.<\/jats:p><\/jats:sec><jats:sec><jats:title>Method<\/jats:title><jats:p>A total of 180 subjects with results of the thalassaemia screen and genetic testing were included. Results of the ICT and HbH preparation were correlated with genetic results to determine the performance characteristics of the tests and the effect of mean sample age on results.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Of 180 subjects, 111 carried alpha thalassaemia mutations and 69 participants had normal genetic results. The ICT had a sensitivity of 63.06% for all alpha gene mutations and 100% for both heterozygous alpha<jats:sup>0<\/jats:sup> and HbH disease, with a specificity of 91.30%. Examination of HbH preparation had a sensitivity of 34.23% overall, detecting 89% of heterozygous alpha<jats:sup>0<\/jats:sup> and 100% of HbH disease with a specificity of 98.55%. Sample age did not affect overall results.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The ICT is a sensitive screening method for significant alpha mutations and detects the majority of homozygous alpha<jats:sup>+<\/jats:sup> and nondeletional mutations. It demonstrates greater correlation with genetic testing than HbH preparation and could replace HbH preparation in the screening algorithm for alpha thalassaemia.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijlh.12994","type":"journal-article","created":{"date-parts":[[2019,3,4]],"date-time":"2019-03-04T17:36:24Z","timestamp":1551720984000},"page":"397-403","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Evaluation of an immunochromatographic test for alpha thalassaemia screening in a multi\u2010ethnic population"],"prefix":"10.1111","volume":"41","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7589-5564","authenticated-orcid":false,"given":"Anna Clare","family":"Nelson","sequence":"first","affiliation":[{"name":"Haematology Department Liverpool Hospital Sydney New South Wales Australia"},{"name":"College of Public Health, Medical &amp; Veterinary Sciences James Cook University Townsville Queensland Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Penelope Irene","family":"Motum","sequence":"additional","affiliation":[{"name":"Haematology Department Liverpool Hospital Sydney New South Wales Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Theophilus I.","family":"Emeto","sequence":"additional","affiliation":[{"name":"College of Public Health, Medical &amp; Veterinary Sciences James Cook University Townsville Queensland Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,3,4]]},"reference":[{"issue":"6","key":"e_1_2_8_2_1","first-page":"417","article-title":"Global epidemiology of haemoglobin disorders and derived service indicators","volume":"86","author":"Modell B","year":"2008","journal-title":"Bull World Health Organ"},{"key":"e_1_2_8_3_1","unstructured":"PatrinosG JolyP WajcmanH MoradkhaniK BorgJ ChuiD.HbVar: A database of human hemoglobin variants and thalassemias.http:\/\/globin.bx.psu.edu\/cgi-bin\/hbvar\/counter."},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/aqx047"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/ejhg.2014.131"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1309\/3VK2-UCJ1-5GBJ-QV8Q"},{"key":"e_1_2_8_7_1","unstructured":"KabralA LiB.Screening for Alpha Thalassaemia: Classical HbH Staining vs i\u2010LAB* Immunochromatographic Method. 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